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Anti cd16 cd32 93

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The Anti-CD16/CD32 (93) is a mouse monoclonal antibody that binds to the Fc gamma receptor III (CD16) and Fc gamma receptor II (CD32) expressed on various cell types, such as monocytes, macrophages, and natural killer cells. This antibody can be used for the identification and enumeration of these cell populations in flow cytometry applications.

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Lab products found in correlation

Ionomycin, by Merck Group (3 mentions) Brefeldin a, by Merck Group (2 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Facs fortessa, by BD (2 mentions) Facsaria, by BD (2 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Ic fixation buffer kit, by Thermo Fisher Scientific (1 mentions) Tnfα mp6 xt22, by Thermo Fisher Scientific (1 mentions) γ xmg1.2, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Golgistop, by BD (1 mentions) Golgiplug, by BD (1 mentions)

Spelling variants of this product

Anti-CD16/CD32 (62 citations) CD16/CD32 (23 citations) Anti-mouse CD16/CD32 (clone 93; (16 citations) CD16/CD32 (93) (6 citations) Anti-CD16/CD32 antibodies (clone 93; (2 citations) Anti-CD16/CD32 (clone 93) (2 citations) Anti-mouse CD16/CD32 mAb (93; (2 citations) Anti-CD16/CD32 antibody clone 93 (2 citations) Anti-CD16/CD32 mAb (93, (2 citations) Anti CD16/CD32 Fc Block (93) (2 citations)

3 protocols using anti cd16 cd32 93

1

Multi-parameter Immune Cell Profiling

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For cell surface staining, thymocytes, erythrocyte-depleted splenocytes or lymph node cells were incubated 30 min with saturating concentrations of mAbs identified above. For intracellular cytokine staining, cells were stimulated with PMA (phorbol 12-myristate 13-acetate) (50ng/mL) and ionomycin (1μg/mL), in the presence of Brefeldin A (10μg/mL) (all from Sigma) for 4h at 37°C. Cells were stained for the identified above cell surface markers, fixed 30min at 4°C and permeabilized with the Foxp3/Transcription Factor Staining Buffer set (eBioscience) in the presence of anti-CD16/CD32 (93) (eBioscience) for 15 min at 4°C, and finally incubated for 1h at room temperature with identified above cytokine-specific Abs in permeabilization buffer. Samples were acquired using FACSFortessa (BD Biosciences). Data were analysed using FlowJo software (Tree Star). Live indicated subsets were electronically sorted when indicated using FACSAria (BD Biosciences).
Muñoz-Ruiz M., Ribot J.C., Grosso A.R., Gonçalves-Sousa N., Pamplona A., Pennington D.J., Regueiro J.R., Fernández-Malavé E, & Silva-Santos B. (2016). TCR signal strength controls thymic differentiation of discrete proinflammatory γδ T cell subsets. Nature immunology, 17(6), 721-727.
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2

Multi-parameter Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
For cell surface staining, thymocytes, erythrocyte-depleted splenocytes or lymph node cells were incubated 30 min with saturating concentrations of mAbs identified above. For intracellular cytokine staining, cells were stimulated with PMA (phorbol 12-myristate 13-acetate) (50ng/mL) and ionomycin (1μg/mL), in the presence of Brefeldin A (10μg/mL) (all from Sigma) for 4h at 37°C. Cells were stained for the identified above cell surface markers, fixed 30min at 4°C and permeabilized with the Foxp3/Transcription Factor Staining Buffer set (eBioscience) in the presence of anti-CD16/CD32 (93) (eBioscience) for 15 min at 4°C, and finally incubated for 1h at room temperature with identified above cytokine-specific Abs in permeabilization buffer. Samples were acquired using FACSFortessa (BD Biosciences). Data were analysed using FlowJo software (Tree Star). Live indicated subsets were electronically sorted when indicated using FACSAria (BD Biosciences).
Muñoz-Ruiz M., Ribot J.C., Grosso A.R., Gonçalves-Sousa N., Pamplona A., Pennington D.J., Regueiro J.R., Fernández-Malavé E, & Silva-Santos B. (2016). TCR signal strength controls thymic differentiation of discrete proinflammatory γδ T cell subsets. Nature immunology, 17(6), 721-727.
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3

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total CNS infiltrates, spleen, and LN cells were washed once in FACS buffer (PBS containing 0.1% BSA) before Fc receptor blocking with anti-CD16/CD32 (93; eBioscience, or 2.4G2; BD PharMingen). Staining of surface molecules was performed at room temperature using antibodies against CD4 (RM4-5), CD8α (53-6.7), CD90.1 (OX-7), α4β7 (LPAM-1, DATK32), CD25 (PC61), CD62L (MEL-14), CD69 (H1.2F3), CD45 (30-F11), CD11b (M1/70), PD1 (J43), CD27 (LG.3A10), and TIGIT (Vstm3; differently conjugated; all eBioscience, BioLegend, or BD) in FACS buffer for 15 min in the dark. Intracellular Foxp3 (FJK-16s, PE- or APC-conjugated; eBioscience) staining was performed using the Foxp3 staining kit (eBioscience) according to the manufacturer’s instructions. Intracellular cytokine staining of splenocytes with APC-conjugated IL-2 (JES6-5H4), IFN-γ (XMG1.2), and IL-10 (JES5-16E3) and PE-conjugated IL-4 (11B11), IL-17 (eBio17B7), GM-CSF (MP1-22E9), and TNFα (MP6-XT22; all eBioscience) was performed after 3–6-h restimulation with T/I (tetradecanoyl phorbol acetate [10 ng/ml; Sigma-Aldrich] plus ionomycin [5 nM; Merck Biosciences]) in the presence of GolgiStop and GolgiPlug (both BD Pharmingen) using the IC Fixation Buffer Kit (eBioscience). Data were acquired on a FACS Canto II (BD Biosciences) flow cytometer and analyzed with FlowJo software (TreeStar).
Xiao Y., Qureischi M., Dietz L., Vaeth M., Vallabhapurapu S.D., Klein-Hessling S., Klein M., Liang C., König A., Serfling E., Mottok A., Bopp T., Rosenwald A., Buttmann M., Berberich I., Beilhack A, & Berberich-Siebelt F. (2020). Lack of NFATc1 SUMOylation prevents autoimmunity and alloreactivity. The Journal of Experimental Medicine, 218(1), e20181853.
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