Mab318

Manufactured by Thermo Fisher Scientific

The MAB318 is a laboratory instrument designed for the detection and analysis of specific biomolecules. It utilizes a monoclonal antibody-based approach to provide accurate and reliable measurements. The core function of the MAB318 is to facilitate the quantification and characterization of target analytes in research and diagnostic applications.

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Lab products found in correlation

A11120, by Merck Group (1 mentions) Ab152, by Merck Group (1 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (1 mentions) Nis element, by Nikon (1 mentions) Csu w1 spinning disk confocal microscope, by Nikon (1 mentions) Fluoromount g with dapi, by Southern Biotech (1 mentions) Anti dsred, by Takara Bio (1 mentions) Cm1950, by Leica camera (1 mentions) C1 confocal microscope, by Nikon (1 mentions)

3 protocols using mab318

Immunostaining of Transgenic Mouse Brains

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Immunostaining of brain sections was used to validate AAV-directed gene targeting or expression (mice were euthanized 2 to 3 weeks after AAV administration) and to image DA neuron neurite density in the NAc. Brain tissues were collected after perfusion with PBS and 4% paraformaldehyde (PFA). PFA-fixed brain tissues were cryostat-sectioned at 40 μm thickness. Sections were permeabilized with 0.5% Triton X-100 for 30 min and then blocked in 5% normal goat serum for 1 hour at room temperature. Free-floating coronal sections were immunostained with anti-Becn2 (Millipore, ABC253), anti-GFP (Invitrogen, A11120), and anti-TH (Millipore, AB152 and MAB318) antibodies and then with Alexa Fluor or Alexa Fluor Plus 488–conjugated secondary antibody (Invitrogen, A-11008, A-11001, and A32723) and Alexa Fluor 594–conjugated secondary antibody (Invitrogen, A-11012 and A-11005). Slides were mounted using ProLong Diamond Antifade Mountant (Invitrogen, P36961). Fluorescence images were acquired using a Nikon CSU-W1 spinning disk confocal microscope. The images were analyzed using Nikon’s NIS-Elements.

Kim Y.J., Kong Q., Yamamoto S., Kuramoto K., Huang M., Wang N., Hong J.H., Xiao T., Levine B., Qiu X., Zhao Y., Miller R.J., Dong H., Meltzer H.Y., Xu M, & He C. (2021). An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system. Science Advances, 7(8), eabc8310.

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Immunohistochemical Labeling of Tyrosine Hydroxylase, ZsGreen, and dsRed in Mouse Brain

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Mice were euthanized and transcardially perfused 4% paraformaldehyde. Brain sections (30μm) were blocked in 0.3% Triton and 3% Normal Donkey Serum (NDS) in 1 × TBS, and then incubated in primary antibodies anti- at 4°C overnight. The slices were then washed with 1 × PBS three times and incubated for 1 hour at room temperature with a secondary antibody. The sections were washed in 1 × PBS three times and mounted and then cover-slipped with Fluoromount-G with DAPI (Southern Biotech). Antibodies: 1) anti-tyrosine hydroxylase (monoclonal, 1:1000; Millipore, MAB318), ZsGreen (rabbit, 1:2000, Invitrogen A11122), and anti-dsRed (polyclonal, 1:1000; Clontech, 632496).

Fellinger L., Jo Y.S., Hunker A.C., Soden M.E., Elum J., Juarez B, & Zweifel L.S. (2021). A midbrain dynorphin circuit promotes threat generalization. Current biology : CB, 31(19), 4388-4396.e5.

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Tissue Preparation and Immunohistochemistry

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The animals were perfused with PBS with heparin followed by 4% paraformaldehyde. Subsequently, livers and brains were removed and transferred to 4% paraformaldehyde followed by 18% and 30% sucrose gradient until equilibrated, as evidenced by sinking to the bottom of tube. Tissue was flash frozen on dry ice. Frozen brains and livers were sectioned with a cryostat (Leica CM1950) at 30 µm. The sections were stained with rabbit anti-GLuc (1:2000; NEB, #E8023S), chicken anti-albumin Sigma SAB3500217 (1:200; Sigma-Aldrich, #SAB3500217; RRID:AB_10640041), mouse anti-tyrosine hydroxylase (1:2000; Millipore, #MAB318; RRID:AB_2201528), chicken anti-calreticulin (1:200; Invitrogen, PA1-902A; RRID:AB_2069607), and rabbit anti-MANF (1:1000; custom polyclonal, Yenzyme, #YZ2155). Nuclei were stained with DAPI (4ʹ,6-Diamidino-2-Phenylindole, Dilactate, 1:3000; Invitrogen, #D3571) then mounted on glass slides with Mowiol. Sections were imaged and captured with a Nikon C1 confocal microscope and processed in Fiji-ImageJ.

Svarcbahs R., Blossom S.M., Baffoe-Bonnie H.S., Trychta K.A., Greer L.K., Pickel J., Henderson M.J, & Harvey B.K. (2023). A transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasis. Transgenic Research, 32(3), 209-221.

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