Anti tmem119 antibody

The primary antibodies included anti-IBA1 antibody (1:100, Wako), anti-CD68 antibody (1: 100, Abcam, Cambridge, MA, United States), anti-CD16/32 antibody (1:100, BD Biosciences), anti-CD206 antibody (1:100, R&D Systems, Inc., Minneapolis, MN, United States), anti-CD11b antibody (1: 100, Abcam, Cambridge, MA, United States), anti-CD86 antibody (1: 100, Abcam, Cambridge, MA, United States), anti-CD40 antibody (1:100, Abcam, Cambridge, MA, United States), anti-CD163 antibody (1:100, Santa Cruz, MA, United States), anti-TMEM119 antibody (1: 100, Abcam, Cambridge, MA, United States), anti-Rhodopsin antibody (1:100, Santa Cruz, MA, United States). These primary antibodies and abbreviates were explained in Supplementary Table S1, including the description of the characteristics of M1 and M2 markers. Secondary antibodies included donkey anti-rabbit IgG (H+L) Alexa Fluor^® 555, goat anti-rat IgG (H+L) Alexa Fluor^® 488, donkey anti-rabbit IgG (H+L) Alexa Fluor^® 488, and donkey anti-goat IgG (H+L) Alexa Fluor^® 555 secondary antibodies (1:800, Invitrogen).

The following primary antibodies were used for Western blot, co-IP, and immunofluorescence: anti-HAX-1 antibody (BD, cat: 610825), anti-NLRP3 antibody (CST, cat: 15101), anti-ASC antibody (CST, cat: 67824), anti-Caspase1 antibody (CST, cat: 24232), anti-Cleaved Caspase1 antibody (CST, cat: 89332), anti-GSDMD antibody (CST, cat:93709), anti-TMEM119 antibody (Abcam, cat: ab209064), anti-β-actin antibody (Abmart, cat: P30002S), Goat anti-rabbit antibody HRP linked (CST, cat: 7074), Goat anti-mouse antibody (CST, cat:7076). The co-IP assays were performed by using Pierce Crosslink Magnetic IP Kit (Thermo Scientific, Cat: 88805). The immunofluorescence assays were performed by using Opal 7-color manual IHC kit (PerkinElmer, NEL801001KT). The following recombinant proteins were used for BLI assay: recombinant NLRP3 (Abmart, cat: EHH22785), recombinant ASC (Proteintech, cat: Ag28424), recombinant HAX-1 (Proteintech, cat: Ag27244). NLRP3 selective inhibitor MCC950 was purchased from APExBIO company, cat: C3780.