Mice were given a single bolus of dexamethasone (Sigma) by ip injection at the concentration of 10 mg/kg or equivalent amount of vehicle (10% DMSO in PBS) at day 10 pi. Neutralizing monoclonal antibodies including 1μg of anti-IL-6 (Biolegend; clone MP5-20F3) or rat IgG1, κ isotype control (Biolegend; clone RTK2071) in 10 μl PBS, and 5μg of anti-FGF-2 (Millipore; clone bFM-1) or mouse IgG1, κ isotype control (Biolegend; clone MOPC-21) in10 μl PBS were administered to mice by subconjunctival injection at days 8, 10, and 12 pi.
Bfm 1
Manufactured by BioLegend
The BFM-1 is a flow cytometry sample mixer designed to ensure consistent mixing of samples prior to analysis. It features a compact and robust design for reliable performance in the laboratory.
Automatically generated - may contain errors
2 protocols using bfm 1
1
Dexamethasone and Antibody Treatments
2
Dexamethasone and Antibody Treatments
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Mice were given a single bolus of dexamethasone (Sigma) by ip injection at the concentration of 10 mg/kg or equivalent amount of vehicle (10% DMSO in PBS) at day 10 pi. Neutralizing monoclonal antibodies including 1μg of anti-IL-6 (Biolegend; clone MP5-20F3) or rat IgG1, κ isotype control (Biolegend; clone RTK2071) in 10 μl PBS, and 5μg of anti-FGF-2 (Millipore; clone bFM-1) or mouse IgG1, κ isotype control (Biolegend; clone MOPC-21) in10 μl PBS were administered to mice by subconjunctival injection at days 8, 10, and 12 pi.
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