Anti glun1

Manufactured by BD

Sourced in Japan

Anti-GluN1 is a laboratory reagent used for the detection and quantification of the GluN1 subunit of the N-methyl-D-aspartate (NMDA) receptor in biological samples. It is a specific antibody that binds to the GluN1 subunit, allowing researchers to study the expression and distribution of this important neuronal receptor.

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Lab products found in correlation

Anti glun2b, by BD (2 mentions) Anti mouse cy3, by Thermo Fisher Scientific (1 mentions) Anti rabbit alexa fluor 647, by Abcam (1 mentions) Anti glun2a, by Merck Group (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Anti glun2a, by Santa Cruz Biotechnology (1 mentions) Anti glua2, by Merck Group (1 mentions) Anti psd 95, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Mouse anti-GluN1 (6 citations) GluN1 (4 citations)

3 protocols using anti glun1

Immunofluorescence Assay for NMDA Receptor Subunits

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For immunofluorescence (IF) assays, coverslips were first fixed with 4% paraformaldehyde–4% sucrose solution for 10 min. Then, cultures were permeabilized in 0.1% Triton X-100–phosphate-buffered saline (PBS) and blocked with 5% normal goat serum in 0.05% Tween-20 PBS (TPBS). After blocking, coverslips were incubated with the primary antibodies for 1 h at 37°C, washed with TPBS, and then incubated with Cy3 and/or Alexa Fluor 647–conjugated secondary antibodies (1:300) for 30 min at 37°C. After washing with TPBS, coverslips were mounted using Mowiol (MOWIOL 4-88 Reagent, Sigma–Aldrich).
The following antibodies were used: anti-GluN1 (mouse monoclonal, 1:300, BD Pharmingen), anti-GluN2A (rabbit polyclonal, 1:100, Millipore), anti-GluN2B (mouse polyclonal, 1:100, BD Pharmingen), anti-rabbit–Alexa Fluor 647 (goat polyclonal, 1:300, Abcam), anti-mouse–Cy3 (goat polyclonal, 1:300, Thermo Fisher Scientific).

Acutain M.F., Griebler Luft J., Vazquez C.A., Popik B., Cercato M.C., Epstein A., Salvetti A., Jerusalinsky D.A., de Oliveira Alvares L, & Baez M.V. (2021). Reduced Expression of Hippocampal GluN2A-NMDAR Increases Seizure Susceptibility and Causes Deficits in Contextual Memory. Frontiers in Neuroscience, 15, 644100.

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Characterization of Platelet-like Particles

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Suspended cells were collected from culture media by centrifugation at 100 g; adherent cells were lifted using 0.05% trypsin in 0.33 mmol L⁻¹ EDTA. NMDAR subunits were tested using the following primary antibodies: anti‐GluN1 (556308, BD Biosciences, San Jose, CA), anti‐GluN2A (sc‐9056, Santa Cruz Biotechnology, Santa Cruz, CA), anti‐GluN2D (sc‐10727, Santa Cruz), as before.37 Anti‐ CD41a and CD61 antibodies (FITC‐ and PE‐conjugated, respectively; BD Biosciences) were diluted 1:20 and incubated with cells for 20 minutes at RT. Ploidy was determined using propidium iodide (PI; 20 μg mL⁻¹; Thermo‐Fisher Scientific).33Platelet–like particles were examined as described.35 Briefly, nucleated cells were collected by centrifugation at 100 g and the supernatant was re‐spun at 1500 g (both for 10 minutes). Pelleted particles were re‐suspended in ice‐cold RPMI‐1640 (supplemented with 10% FBS and 0.02% sodium azide) to 2 × 10⁵ mL⁻¹. Anti‐ CD41a‐FITC and CD61‐PE antibodies were incubated as above.

Kamal T., Green T.N., Hearn J.I., Josefsson E.C., Morel‐Kopp M., Ward C.M., During M.J, & Kalev‐Zylinska M.L. (2017). N‐methyl‐d‐aspartate receptor mediated calcium influx supports in vitro differentiation of normal mouse megakaryocytes but proliferation of leukemic cell lines. Research and Practice in Thrombosis and Haemostasis, 2(1), 125-138.

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Antibody Characterization for Glutamate Receptor Subunits

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Anti-GluK3 antibody raised in rabbit against C-terminal 17 amino acid residues of mouse GluK3 (903–919, NM_001081097) was used for immunohistochemistry, immunoprecipitation, and western blotting. The rabbit polyclonal antibodies anti-GluK2 (Synaptic Systems, 180 003), anti-GluK4^{41 (link)}, and anti-GluK5 (Millipore, 06-315) were used for immunoprecipitation and western blotting. The specificities of these antibodies have been determined previously^{29 (link)}. Anti-GluN1 (BD Biosciences, 556308), anti-GluN2A (BD Biosciences, 612286), anti-GluN2B (BD Biosciences, 610416), anti-GluA1 (Frontier Institute, Japan, MSFR102270), anti-GluA2 (Millipore, MAB397), anti-GluA3^{44 (link)}, anti-PSD-95 (Santa Cruz Biotechnology, SC32290), anti-D1R (Frontier Institute, Japan, MSFR101030), and anti-D2R (Frontier Institute, Japan, MSFR101060) were used for western blotting.

Iida I., Konno K., Natsume R., Abe M., Watanabe M., Sakimura K, & Terunuma M. (2024). Behavioral analysis of kainate receptor KO mice and the role of GluK3 subunit in anxiety. Scientific Reports, 14, 4521.

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