Dab chromogen universal lsab kits system hrp

For immunohistochemical analysis, brain tissue was fixed in 4% phosphate-buffered formalin for 5–6 weeks and subsequently embedded in paraffin. From the paraffin-embedded tissue blocks, 4 μm thin sections were put on X-tra Adhesive Precleaned Micro Slides (Leica) and exposed to a mouse monoclonal anti-Aβ antibody (clone 6E10; 1:1000, BioLegend (previous Covance); as described by Sudduth et al., 2013 (link)), rabbit polyclonal anti-GFAP antibody (1:100, abcam; as described by Lu et al., 2010 (link)) and rabbit polyclonal anti-NeuN antibody (1:1000, abcam, as described by Park et al., 2016 (link)). For the development DAB chromogen Universal LSAB® kits (System-HRP; DakoCytomation, Dako) were used according to the manufacturer's instructions. The sections were counterstained with hemalaun and analyzed with a light microscope (Olympus BX51). Images were acquired with a Color View II FW camera (Color View). Within the hippocampus (n = 4 of each mouse strain, n = 25 of visual fields) and cortex (n = 4 of each mouse strain, n = 40 of visual fields), the number of anti-Aβ positive plaques, anti-GFAP and anti-NeuN positive cells were counted and given as number per mm².