Bufalin, Doxorubicin (DOX), Rho-123, verapamil and Lucifer yellow were obtained from Sigma–Aldrich Chemical Co. (St. Louis, MO, USA). Minimum Essential Media (MEM), fetal bovine serum (FBS), non-essential amino acids (NEAA), Ham's F-12K (Kaighn’s) Medium (F12K), and Hank's balanced salt solution (HBSS), were obtained from Gibco BRL (Carlsbad, CA, USA). RPMI 1640 and phosphate-buffered saline (PBS) were from Hyclone (Thermo Scientific, Logan, UT). Annexin V-FITC Apoptosis Detection Kit was purchased from BD Biosciences (Beijing, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). A ABCB1 ATPase assay system was purchased from Promega (Madison, WI, USA). The primary antibodies for ABCB1 (#13342) and β-actin (#3700) were sourced from Cell Signaling Technology (Boston, USA). The secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Abcb1 atpase assay system
Manufactured by Promega
Sourced in United States
The ABCB1 ATPase assay system is a laboratory tool designed to measure the ATPase activity of the ABCB1 (P-glycoprotein) transporter. The assay provides a quantitative evaluation of the interaction between ABCB1 and test compounds. The core function of this product is to enable the assessment of ABCB1 activity in a controlled experimental setting.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Abcb1, by Cell Signaling Technology (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
Rho123, by Merck Group (1 mentions)
Doxorubicin dox, by Merck Group (1 mentions)
Lucifer yellow, by Merck Group (1 mentions)
Bufalin, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Verapamil, by Merck Group (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
V fitc apoptosis detection kit, by BD (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
2 protocols using abcb1 atpase assay system
1
Multidrug Resistance Mechanisms Evaluation
2
Evaluating ABCB1-Mediated Drug Resistance
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BUF was obtained from Sigma (Saint Louis, USA). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). The ABCB1 ATPase assay system was purchased from Promega (Madison, WI, USA). The primary antibodies for ABCB1 (#13342) and β-actin (#3700) were sourced from Cell Signaling Technology (Boston, USA). RPMI 1640, DMEM and PBS were purchased from HyClone (Thermo Scientific, Logan, UT). For cell culture, 0.02% EDTA, 0.25% trypsin, penicillin–streptomycin and FBS were obtained from Gibco BRL (Carlsbad, CA, USA). The V-FITC Apoptosis Detection Kit was purchased from BD Biosciences (Beijing, China).
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