Anti nfatc1 sc 7294

Dulbecco’s modified Eagle’s medium GlutaMAX (DMEM), fetal bovine serum (FBS), penicillin, streptomycin, protease and phosphatase inhibitors cocktail were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Recombinant human sRANKL (Invitrogen_PHP0034) was purchased from Thermo Fisher Scientific. Anti-NFATc1 (sc-7294) and anti-c-Fos (sc-166940) polyclonal antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Anti-phospho-Smad1/5/9 and anti-GAPDH antibodies were obtained from Cell Signaling (Cell Signaling Technology, Danvers, MA, USA). Primers for RT-PCR (QuantiTect primer assay, Table S1) and RNA extraction kit (RNeasy kit) were purchased from Qiagen (Hilden, Germany). The primers for Cathepsin K and c-Fos were designed as already described [10 (link)] and ordered from Microsynth (Balgach, Switzerland). TRAP staining solution (leukocyte acid phosphatase kit, 386-A) was purchased from Sigma. All others chemicals were obtained from Sigma (St. Louis, MO, USA).