Western blot analysis was performed as previously described (Dorsey et al., 2012 (link)). Briefly, 20 μ g of clarified muscle extract was subjected to SDS-PAGE, transferred to nitrocellulose membranes and blocked in a 5% milk solution in PBS for 1 h. The membrane was probed overnight with primary antibody at room temperature. The primary antibodies were anti-α-tubulin (DM1A, 1:1000; Sigma-Aldrich), anti-β-tubulin (AA12.1, 1:1000, Developmental Studies Hybridoma Bank), anti-Glu-tubulin (1:1000, Millipore, Temecula, CA, USA), anti-gp91phox (1:1000, BD Transduction Laboratories, Lexington, KY, USA), and anti-GAPDH (1:4000, Millipore). Membranes were washed twice for 10 min in 5% milk solution at room temperature, then incubated with appropriate secondary antibody (1:10,000) for 1 h at room temperature and washed in a 0.5% Tween solution in PBS twice for 10 min. Immunoblots were developed using SuperSignal PicoWest chemiluminescence (Pierce, Rockford, IL, USA) and were imaged and quantified with a SYNGENE GBox imaging system (GeneTools software). GAPDH served as a loading control.
Anti gp91phox
Manufactured by BD
Sourced in United States
Anti-gp91phox is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to the gp91phox protein, which is a component of the NADPH oxidase complex. The core function of Anti-gp91phox is to enable the detection and study of the gp91phox protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Merck Group (1 mentions)
Anti α tubulin dm1a, by Merck Group (1 mentions)
Anti cd11b, by Bio-Rad (1 mentions)
Anti arginase 1 arg1, by Abcam (1 mentions)
Anticholine acetyltransferase, by Abcam (1 mentions)
Anti cd163, by Santa Cruz Biotechnology (1 mentions)
Anti akt, by Santa Cruz Biotechnology (1 mentions)
Anti smi32, by Fortrea (1 mentions)
2 protocols using anti gp91phox
1
Western Blot Analysis of Muscle Proteins
2
Histological Analysis of Immune Markers
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Reagents were purchased from Sigma (Italy). Antibodies raised in rabbit: anti‐arginase 1 (ARG1) (1:100, Abcam, UK); anti‐CD163 (1:100, Santa Cruz Biotechnology, USA); anti‐choline acetyltransferase (1:500, AbCam); anti‐DAO1 (1:200, Bioss, USA); anti‐H1R (1:200, Alomone, Israel); anti‐H2R (1:200, MyBioSource, USA); anti‐H3R (1:200, Alomone); anti‐H4R (1:200, Santa Cruz Biotechnology); anti‐HDC (1:20, AbCam); anti‐HNMT (1:200, Sigma); anti‐inducible nitric oxide synthase (iNOS) (1:1000, CST, USA); anti‐myelin basic protein (MBP) (1:1000 CST); anti‐nuclear factor‐kappa B (NF‐κB) p65 (1:500, CST); anti‐phospho‐AKT (1:500, CST); anti‐phospho‐p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (1:1000, CST); and anti‐phospho‐NF‐κB p65 (Ser536) (1:500, CST). Antibodies raised in mouse: anti‐AKT (1:500, Santa Cruz Biotechnology); anti‐GAPDH (1:2500, Calbiochem, USA); anti‐gp91phox (1:1000, BD Transduction Laboratories, USA); anti‐SMI32 (1:1000, Covance, USA); and anti‐p44/42 MAPK (ERK1/2) (L34F12) (1:1000, CST). Antibodies raised in rat: anti‐CD11b (1:200, AbD Serotec, UK).
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