Monoclonal anti-LDLR and polyclonal anti-Sp1 antibodies were purchased from Millipore. Polyclonal anti-phospho-ERK1/2 and anti-ERK1/2 antibodies were purchased from Cell Signaling Technology. Polyclonal anti-phospho-Sp1 (Thr-453) antibody was purchased from Abcam and polyclonal anti-phospho-Sp1 (Thr-739) antibody was purchased from LifeSpan BioSciences. Monoclonal anti-β-actin (AC-15) antibody was purchased from Sigma. Peroxidase-conjugated affinity-purified goat anti-rabbit and goat anti-mouse IgGs were purchased from Jackson Immunoresearch Laboratories.
Anti β actin monoclonal antibody ac 15
Manufactured by Merck Group
Sourced in United States
The Anti-β-actin monoclonal antibody (AC-15) is a laboratory reagent used to detect the presence of the β-actin protein in biological samples. β-actin is a highly conserved cytoskeletal protein found in all eukaryotic cells and is commonly used as a reference or housekeeping protein in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Peroxidase conjugated affinity purified goat anti rabbit and goat anti mouse iggs, by Jackson ImmunoResearch (2 mentions)
Inhibitor of ap 1 sr11302, by R&D Systems (2 mentions)
Anti st2 polyclonal antibody, by Santa Cruz Biotechnology (2 mentions)
Anti nf κb p65 monoclonal d14e12, by Cell Signaling Technology (2 mentions)
Anti tata binding protein polyclonal antibody, by Merck Group (2 mentions)
Bay11 7982, by Merck Group (2 mentions)
Recombinant human il 33, by R&D Systems (2 mentions)
Sb203580, by Merck Group (2 mentions)
Sp600125, by Merck Group (2 mentions)
Goat anti rabbit igg, by Merck Group (2 mentions)
Anti erk1 2 antibody, by Cell Signaling Technology (1 mentions)
Monoclonal anti srebp 1 2a4, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg antibody, by Merck Group (1 mentions)
Anti p53 monoclonal antibody 1c12, by Cell Signaling Technology (1 mentions)
Anti flag m2 monoclonal antibody, by Merck Group (1 mentions)
Goat anti mouse igg antibody, by Merck Group (1 mentions)
Anti egfr antibody, by Cell Signaling Technology (1 mentions)
Erlotinib, by Cayman Chemical (1 mentions)
Chemidoc xrs plus, by Bio-Rad (1 mentions)
Clarity western ecl substrate, by Bio-Rad (1 mentions)
7 protocols using anti β actin monoclonal antibody ac 15
1
Antibody Characterization for Western Blot
2
Antibodies for Protein Analysis
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Monoclonal anti-LDLR antibody (C7) was obtained from Millipore, and monoclonal anti-SREBP–1 (2A4) and anti-SREBP–2 (1C6) antibodies were obtained from Santa Cruz. Monoclonal anti-β-actin (AC–15) antibody was purchased from Sigma, and the anti-SREBP–2 polyclonal antibody (Rs004) was used as described previously [21 (link)]. Peroxidase-conjugated affinity-purified goat anti-rabbit and goat anti-mouse IgGs were purchased from Jackson Immunoresearch Laboratories.
3
Antibody Characterization for Cell Analysis
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Antibodies used in this study were listed as follows: anti-p53 monoclonal antibody (1C12, Cell Signaling Technology), anti-p53 monoclonal antibody (7F5, Cell Signaling Technology), anti-Flag monoclonal antibody (M2, Sigma), anti-NS1 monoclonal antibody (NS1-23-1, Santa Cruz), anti-MDM2 polyclonal antibody (C18, Santa Cruz), anti-RPL11 polyclonal antibody (SAB2700779, Sigma), anti-RPL23 polyclonal antibody (I-20, Santa Cruz), anti-nucleolin monoclonal antibody (C23, Santa Cruz), anti-nucleolin polyclonal antibody (N2662, Sigma), anti-RPA194 monoclonal antibody (C-1, Santa Cruz), anti-β-actin monoclonal antibody (AC-15, Sigma), a horseradish peroxidase (HRP) conjugated goat anti mouse IgG antibody (Sigma), goat anti-rabbit IgG antibody (Sigma).
4
Signaling Pathway Inhibition in Cell Lines
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Recombinant human IL-33 and inhibitor of AP-1 (SR11302) were obtained from R&D Systems (Minneapolis, MN, USA). Anti-ST2 polyclonal antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-ERK1/2 monoclonal (D13.14.4E), anti-ERK1/2 monoclonal (137F5), anti-phospho-p38 MAPK monoclonal (D3F9), anti-p38 MAPK polyclonal, anti-phospho-JNK monoclonal (81E11), anti-JNK polyclonal, anti-phospho-c-Fos monoclonal (D82C12), anti-c-Fos monoclonal (9F6), anti-phospho-c-Jun monoclonal (54B3), anti-c-Jun monoclonal (60A8), anti-IκB-α monoclonal (L35A5), and anti-NF-κB p65 monoclonal (D14E12) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin monoclonal antibody (AC-15) and anti-TATA binding protein (TBP) polyclonal antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Proteintech Group (Rosemont, IL, USA), respectively. Specific inhibitors of p38 MAPK (SB203580), JNK (SP600125), and NF-κB (BAY11-7982) were purchased from Merck Millipore (Darmstadt, Germany).
5
Signaling Pathway Inhibition in Cell Lines
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Recombinant human IL-33 and inhibitor of AP-1 (SR11302) were obtained from R&D Systems (Minneapolis, MN, USA). Anti-ST2 polyclonal antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-ERK1/2 monoclonal (D13.14.4E), anti-ERK1/2 monoclonal (137F5), anti-phospho-p38 MAPK monoclonal (D3F9), anti-p38 MAPK polyclonal, anti-phospho-JNK monoclonal (81E11), anti-JNK polyclonal, anti-phospho-c-Fos monoclonal (D82C12), anti-c-Fos monoclonal (9F6), anti-phospho-c-Jun monoclonal (54B3), anti-c-Jun monoclonal (60A8), anti-IκB-α monoclonal (L35A5), and anti-NF-κB p65 monoclonal (D14E12) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-β-actin monoclonal antibody (AC-15) and anti-TATA binding protein (TBP) polyclonal antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Proteintech Group (Rosemont, IL, USA), respectively. Specific inhibitors of p38 MAPK (SB203580), JNK (SP600125), and NF-κB (BAY11-7982) were purchased from Merck Millipore (Darmstadt, Germany).
6
Gliadin-Induced Intestinal Permeability
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Gliadin from wheat was purchased from Tokyo Chemical Industry (Tokyo, Japan). Indomethacin, diclofenac, zein, and Evans blue were purchased from Wako Pure Chemical Inc. (Osaka, Japan). Fluorescein isothiocyanate (FITC)-dextran (average molecular weight 3000–5000) (#FD4) and monoclonal anti-β-actin antibody (#AC-15) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Erlotinib, a tyrosine kinase inhibitor that acts on EGFR, was purchased from Cayman Chemical (Ann Arbor, MI, USA). Anti-EGFR antibody and anti-phosphorylated-EGFR (Tyr1068) (p-EGFR) antibody were purchased from Cell Signaling Technology (Beverly, MA, USA).
7
Hepatic Protein Immunoblotting Protocol
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The hepatic S9 and mitochondrial fractions were prepared as described previously (Miyata et al., 2011) . Goat anti-Scd1 antibody (S-15: Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-Fas antibody (H300: Santa Cruz Biotechnology), rabbit anti-Cpt2 antibody (EPR13626: Abcam, Cambridge, UK), rabbit anti-Cpt1A antibody (15184-1-AP: Proteintech Group Inc., Chicago, IL, USA), rabbit anti-Cox IV antibody (11242-1-AP: Proteintech Group Inc.) and monoclonal anti-β-actin anti-body (AC-15: Sigma-Aldrich, St. Louis, MO, USA) were used as primary antibodies. Goat anti-mouse IgG (H + L), horseradish peroxidase-conjugated (Thermo Scientific, Waltham, MA, USA), rabbit anti-goat IgG (whole molecule), horseradish peroxidase-conjugated (Sigma-Aldrich) and goat anti-rabbit IgG (whole molecule) antibodies were used as secondary antibodies. Detection of immunoblot signalings was performed using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) and ChemiDoc XRS Plus (Bio-Rad).
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