Spleen and bone marrow cells (4 x 106) were disaggregated into single-cell suspensions, treated with RBC lysis buffer (eBioscience) then stimulated with PMA (25 ng/ml) and ionomycin (1 µg/ml) (Sigma). After stimulation, the cells were stained with NK-1.1-allophycocyanin (PK136), CD3-AlexaFluor700 (17A2), CD8α-FITC (53–6.7) and CD4-PE-CF594 (RM4–5) (all from BD Biosciences) for 30 min. The cells were permeabilized in Perm/Fix buffer (BD Biosciences) then stained with IFNγ-PE (XMG1.2) (BD Biosciences). All cells were fixed in 1% paraformaldehyde before analysis with an LSR-II flow cytometer (BD Biosciences). Up to 500,000 events per sample was captured for data analysis using the FlowJo software (Treestar). Isotype controls (BD Biosciences) were used for gating.
Ifnγ pe xmg1.2
Manufactured by BD
Sourced in United States
IFNγ-PE (XMG1.2) is a fluorochrome-conjugated monoclonal antibody that binds to interferon-gamma (IFNγ). This product is designed for flow cytometric analysis of IFNγ-producing cells.
Automatically generated - may contain errors
Lab products found in correlation
Lsrii flow cytometer, by BD (1 mentions)
Isotype control, by BD (1 mentions)
Fix perm buffer, by BD (1 mentions)
Rbc lysis buffer, by Thermo Fisher Scientific (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Cd4 pecf594 rm4 5, by BD (1 mentions)
Tnf α fitc mp6 xt22, by Thermo Fisher Scientific (1 mentions)
2 protocols using ifnγ pe xmg1.2
1
Multiparametric Flow Cytometry of Immune Cells
2
Multiparametric Flow Cytometry Panel
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The following fluorescence conjugated anti-mouse mAbs were purchased from eBioscience (San Diego, CA, USA): CD4-APC (L3T4), CD11c-APC and PE-Cy7 (N418), CD44-PE (IM7), CD86-PE (GL1), MHC Class II-FITC (M5/114.15.2), NK1.1-PE-cy7 (PK136), IgA-FITC (mA-6E1), IgM-APC (II/41) and TNF-α-FITC (MP6-XT22). B220-PerCP and APC-Cy7 (RA3-6B2), CD3ε-PerCP (145-2C11), CD8α-PerCP (53-6.7), CD11b-APC-Cy7 (M1/70), IFN-γ-PE (XMG1.2) and IL-12p40/p70-PE (C15.6) were purchased from BD Pharmingen (San Diego, CA, USA). PDCA-1-APC (JF05-1C2.4.1) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
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