Atm s1981

Manufactured by Abcam

Sourced in United States

The ATM-S1981 is a laboratory equipment product. It serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

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Lab products found in correlation

Close spelling variants of this product

Anti-phospho-ATM (S1981) (6 citations) Rabbit polyclonal antibody against phospho-ATM (S1981) (2 citations) Phosphorylated S1981 ATM (2 citations)

4 protocols using atm s1981

Comprehensive Antibody Panel for DNA Damage Response

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Primary antibodies used were from Cell Signalling unless otherwise mentioned: β-actin (Abcam #ab6276), cleaved PARP (#5625), CDK1 Y15 (#9111), CHK1 (#2360), CHK1 S345 (#2348), H2AX (#7631), H2AX S139 (Millipore #05-636), H3 (#9715), H3 S10 (#3377), RRM2 (ABNOVA #H00006241-M01), ATR (Santa Cruz #1887), ATR T1989 (Gene Tex #GTX128145), CHK2 (#2662), CHK2 T68 (Abcam #3501), KAP1 (Abcam #10484), KAP1 S824 (Abcam #133440), DNA-PKcs (Abcam #70250), DNA-PKcs S2056 (Abcam #18192), ATM (Abcam #78), ATM S1981 (Abcam #81292), RPA32 (Abcam #2175), RPA32 S4/8 (Bethyl Laboratories #A300-245A), RPA32 S33 (Bethyl Laboratories #A300-246A).
For secondary antibodies, Alexa 488 (#4408, #4412) and Alexa 647 (#4410, #4414) from Cell Signalling were used in immunostaining. IRDye800-conjugated (#925-32210, #926-33210) and IR680-conjugated (#926-68070, #926-68021) antibodies from LICOR were used in immunoblotting.

Wallez Y., Dunlop C.R., Johnson T.I., Koh S.B., Fornari C., Yates J.W., Bernaldo de Quirós Fernández S., Lau A., Richards F.M, & Jodrell D.I. (2018). The ATR inhibitor AZD6738 synergizes with gemcitabine in vitro and in vivo to induce pancreatic ductal adenocarcinoma regression. Molecular cancer therapeutics, 17(8), 1670-1682.

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DNA Damage Response Protein Analysis

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Cells were harvested in UTB lysis buffer (9 M Urea, 75 mM Tris-HCl pH 7.5, 0.15 M β-mercaptoethanol). Blots were visualised using the Odyssey Infrared imaging system. Antibodies used were SETX, KAP1 (Bethyl); GRP78, HIF1α, (ΒD Biosciences); ATM-S1981, RPA-S4/8, β-Tubulin (Abcam); ATM, RPA, KAP1-S824, p53-S15, H3K9me2, H3K4me2, H3K9Ac, H3-S10, H3, H3K36me3, Chk1-S317, PERK (Cell Signaling); p53, β-actin, RNase H1, Chk1 (Santa Cruz); and H3K9me3, γH2AX, H2AX (Millipore).

Ramachandran S., Ma T.S., Griffin J., Ng N., Foskolou I.P., Hwang M.S., Victori P., Cheng W.C., Buffa F.M., Leszczynska K.B., El-Khamisy S.F., Gromak N, & Hammond E.M. (2021). Hypoxia-induced SETX links replication stress with the unfolded protein response. Nature Communications, 12, 3686.

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Immunofluorescence and Western Blot Antibodies

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The following antibodies were used in this study: ATM (catalog no. 2873; Cell Signaling Technology), ATM-S1981 (catalog no. ab81292; Abcam), CHK2 (catalog no. 05-649; Millipore), CHK2-T68 (catalog no. 2661; Cell Signaling Technology), eGFP (catalog no. ab6556; Abcam), γ-H2AX (catalog no. 05-636; Millipore), H2B (catalog no. 8135; Cell Signaling Technology), mCherry (catalog no. STJ34373; St. John’s Laboratory), Vinculin (catalog no. V9131; Sigma), 53BP1 (catalog no. Sc-22760; Santa Cruz), goat anti-mouse Alexa 488 conjugate (catalog no. A11031; ThermoFisher), goat anti-rabbit Alexa 568 conjugate (catalog no. A-11011; ThermoFisher), polyclonal goat anti-rabbit immunoglobulins HRP-conjugate (catalog no. P0448; Dako), and polyclonal goat anti-mouse immunoglobulins HRP-conjugated (catalog no. P0447; Dako).

Lou J., Scipioni L., Wright B.K., Bartolec T.K., Zhang J., Masamsetti V.P., Gaus K., Gratton E., Cesare A.J, & Hinde E. (2019). Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response. Proceedings of the National Academy of Sciences of the United States of America, 116(15), 7323-7332.

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Antibody Sources and Dilutions

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The sources and dilutions of antibodies used were as follows: BrdU (Life Technologies), 53BP1 (polyclonal; Novus, Littleton, CO, USA), γH2AX (Upstate, Chicago, IL, USA); α-Tubulin, p53(S15), Chk2(T68), ATR(S428) (Cell Signalling, Danvers, MA, USA); ATM(S1981) (Abcam, Cambridge, MA, USA); p21 (Santa Cruz, Dallas, TX, USA).

Boccardi V., Razdan N., Kaplunov J., Mundra J.J., Kimura M., Aviv A, & Herbig U. (2015). Stn1 is critical for telomere maintenance and long-term viability of somatic human cells. Aging Cell, 14(3), 372-381.

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