Ot 2 mice

Manufactured by Charles River Laboratories

Sourced in France

The OT-II mice are a transgenic mouse model that expresses a T cell receptor (TCR) specific for a peptide derived from chicken ovalbumin. These mice are commonly used in immunological research to study T cell activation and differentiation.

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Lab products found in correlation

Nr2f6 ot 2, by Charles River Laboratories (1 mentions) C57bl 6 tg tcratcrb 425cbn crl nr2f6 ot 2 mice, by Charles River Laboratories (1 mentions) Batf3 mice 013755, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Charles River Laboratories (1 mentions) C57bl 6j, by Charles River Laboratories (1 mentions) Dermatophagoides pteronyssinus, by Stallergenes Greer (1 mentions)

5 protocols using ot 2 mice

Transcription Factor Nr2f6 Deficient Mice

Cited 3 times

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Nr2f6-deficient mice on the C57BL/6 background (Hermann-Kleiter et al., 2008 (link), 2012 (link), 2015 (link)), Nr2f6^+/+-OT-II (C57BL/6-Tg(TcraTcrb) 425Cbn/Crl; Nr2f6^+/+-OT-II mice) purchased from Charles River and crossed into the Nr2f6-deficient background (Nr2f6^−/− -OT-II mice). B6.SJL (CD45.1) on a C57BL/6 background, or B6.SJL crossed to C57BL/6 (CD45.1/CD45.2) were used for adoptive transfer experiments. Mice were maintained under specific pathogen-free conditions (SPF following FELASA guidelines) and used at the age of 6-14 weeks of age (with the exception of the memory experiments). All animal experiments were performed in accordance with national and European guidelines and reviewed and authorized by the committee on animal experiments (BMWFW-66.011/0064-WF/V/3b/2016; BMWFW-66.011/0112-WF/V/3b/2017).

Olson W.J., Jakic B., Labi V., Schoeler K., Kind M., Klepsch V., Baier G, & Hermann-Kleiter N. (2019). Orphan Nuclear Receptor NR2F6 Suppresses T Follicular Helper Cell Accumulation through Regulation of IL-21. Cell Reports, 28(11), 2878-2891.e5.

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Athymic Nude Mouse Maintenance Protocol

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Six- to eight-week-old female foxn1-null nude (athymic nude) mice were purchased from the Duke University Division of Laboratory Animal Resources (originally obtained from Jackson Laboratory, 007850). Six- to eight-week-old female C57BL/6, OTI (on a C57BL/6 background), and OTII mice (on a C57BL/6 background) were purchased from Charles River Laboratories. Six- to eight-week-old female Batf3^−/− mice (013755) ^{31 (link)} were purchased from Jackson Laboratory. All mice were housed at an ambient temperature of 72°F (~22.22°C), with a humidity of 30–70%, and a light cycle of 12-hour on/12-hour off set from 7am to 7pm. All mice were maintained under specific pathogen-free conditions and animal procedures were reviewed and approved by the Duke University Animal Care and Use Committee. Animal care was carried out under institutional and National Institutes of Health protocols and guidelines.

Huang D., Wang Y., Thompson J.W., Yin T., Alexander P.B., Qin D., Mudgal P., Wu H., Liang Y., Tan L., Pan C., Yuan L., Wan Y., Li Q.J, & Wang X.F. (2022). Cancer Cell-Derived GABA Promotes β-Catenin-Mediated Tumor Growth and Immunosuppression. Nature cell biology, 24(2), 230-241.

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Ovariectomy and Bone Analysis in Mice

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OT-II mice were purchased from Charles River Laboratory and C57BL/6 Cx3cr1^GFP/+ mice were kindly provided by F. Laurent (INRA, Nouzilly, France). Animals were maintained under a 12 hr light/12 hr dark cycle with free access to water and standard mouse diet. For ovariectomy, female mice were randomly divided into 2 groups, which were then sham-operated (SHAM) or ovariectomized (OVX). Ovariectomy and sham surgery were performed on 6 weeks old Cx3cr1^GFP/+ and Cx3cr1^GFP/GFP female mice after anesthesia with isoflurane. The animals were intensively monitored for 72 hr after the procedure. Animals were weighed at the beginning of the study and twice a week thereafter. Six weeks after ovariectomy, mice were sacrificed. Uteri were weighed to control the quality of ovariectomy (not shown). Bones were used either for bone marrow cell isolation and further flow cytometry analysis or for subsequent µCT and histological analysis. Approval for animal experiments was obtained from the Institutional Ethics Committee on Laboratory Animals (CIEPAL-Azur, Nice Sophia-Antipolis, France) and experiments were conducted in compliance with ethical regulations for animal testing and research.

Madel M.B., Ibáñez L., Ciucci T., Halper J., Rouleau M., Boutin A., Hue C., Duroux-Richard I., Apparailly F., Garchon H.J., Wakkach A, & Blin-Wakkach C. (2020). Dissecting the phenotypic and functional heterogeneity of mouse inflammatory osteoclasts by the expression of Cx3cr1. eLife, 9, e54493.

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Murine Immune Response Study

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Adult (8-week-old) female C57BL/6J, male DBA/2J, and male Balb/c strains of mice were purchased from the Experimental Medicine Center, Medical University of Bialystok (Poland). OT-II mice were purchased from Charles River Laboratories (France). The animals were housed in a constant light-to-dark ratio of 12 : 12 hours under specific pathogen free (SPF) conditions. All described procedures were approved by the Local Ethics Committee of the Institute of Immunology and Experimental Therapy in Wroclaw (permission number 4/2009).

Maj T., Slawek A, & Chelmonska-Soyta A. (2014). CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4+ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice. Mediators of Inflammation, 2014, 769239.

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Hypoxic Asthma Model in Mice

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C57BL/6J and OT‐II mice were obtained from Charles River (Sulzfeld, Germany). Mice were maintained in isolated ventilated cages with 12 h light/dark cycles. Water and chow were supplied ad libitum. All mouse experiments met EU guidelines 2010/63/EU and were approved by the Federal Ministry of Science, Research and Economics, Vienna, Austria. All measures were taken to keep animal suffering to a minimum. HDM extract was obtained from Greer Laboratories Inc. (Dermatophagoides Pteronyssinus, lyophilized; lot XPB82D3A25), containing: (23.4% total protein, 0.80% DerP1, 0.02% Endotoxin). Mice were treated intra‐nasally with a crude extract of HDM (50 µg protein/25 µl in PBS) once per week for 5 weeks, control mice received PBS. Two groups of mice additionally were subjected to reduced oxygen levels (10% normobaric oxygen) for the last 2 weeks of HDM treatment (Figure 1A). Analysis of lung function parameters and organ collection was performed 72 h after the last challenge.

Hochgerner M., Sturm E.M., Schnoegl D., Kwapiszewska G., Olschewski H, & Marsh L.M. (2021). Low oxygen levels decrease adaptive immune responses and ameliorate experimental asthma in mice. Allergy, 77(3), 870-882.

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