The formalin fixed and paraffin-embedded liver tissue slices (5 μm thickness) were deparaffinized and rehydrated for histopathological evaluation [21 (link),22 (link)]. For sirius red staining, the sections were stained by hematoxylin and sirius red. For immunohistochemical staining, the sections were incubated with 0.3% hydrogen peroxide/phosphate-buffered saline for 30 min and blocked with 10% BSA (Sangon, AD0023-100). Slides were first incubated using the antibody for CTHRC1 (Huabio), α-SMA (Sigma, A5228) or desmin (Abcam, ab15200) at 4 °C overnight with optimal dilution, labeled by HRP second antibody of mouse (Cell Signaling, 5470S) or rabbit (Abcam, ab136817) at room temperature for 1 h. Then the sections were treated with DAB substrate liquid (Thermo, S21024-2) and counterstained by hematoxylin. All the sections were observed and photographed with a microscope (Carl Zeiss).
S21024 2
Manufactured by Thermo Fisher Scientific
The S21024-2 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is a device designed for use in research and scientific applications, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Additional information may be available from the manufacturer.
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2 protocols using s21024 2
1
Histopathological Evaluation of Liver Tissue
2
IHC Staining of Liver Tissue
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IHC staining was performed as described previously (27 (link)). Briefly, the formalin-fixed and paraffin-embedded liver tissue slides were deparaffinized and rehydrated for histopathological evaluation. The sections were blocked in 10% BSA and then incubated with primary antibodies (TIM, Abcam ab72458; CD68, Servicebio, GB113150) overnight at 4°C and with secondary antibodies for 1 h at room temperature. Then the sections were treated with DAB substrate liquid (Thermo, S21024-2) and counterstained by hematoxylin. All the sections were observed and photographed with a microscope (Carl Zeiss).
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