Harris hematoxylin

Manufactured by StatLab

Sourced in United States

Harris hematoxylin is a staining reagent used in histology and cytology. It is a complex dye that selectively binds to nucleic acids, allowing the visualization of cell nuclei in tissue sections or cytological preparations.

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Lab products found in correlation

Eosin y phyloxine b solution, by Electron Microscopy Sciences (2 mentions) Eosin y, by StatLab (2 mentions) Prolong glass antifade mountant with nucblue, by Thermo Fisher Scientific (1 mentions) Lsm 510 meta, by Zeiss (1 mentions) Bx41 microscope, by Olympus (1 mentions) Bx41 scope, by Olympus (1 mentions) Cytation 5, by Agilent Technologies (1 mentions) Jb 4 resin, by Polysciences (1 mentions) Provis ax70 microscope, by Olympus (1 mentions) Poly mount, by Polysciences (1 mentions) 3 3 diaminobenzidine substrate kit, by Vector Laboratories (1 mentions) Bx51 microscope, by Olympus (1 mentions) Vectastain elite abc kit, by Vector Laboratories (1 mentions) Immpress polymerized reporter enzyme staining system, by Vector Laboratories (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Dab peroxidase substrate kit, by Vector Laboratories (1 mentions) Edta solution, by Merck Group (1 mentions) Ts2r fl microscope, by Nikon (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

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Hematoxylin (14 citations)

8 protocols using harris hematoxylin

Semi-Quantitative Histopathological Lung Scoring

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All lung lobes from each set of four animals from all groups not lavaged were embedded in paraffin and used for histological analysis. Sections (5 µm thick) were stained with hematoxylin and eosin stain (H&E, Harris Hematoxylin, and Eosin Y Stain: American MasterTech, Lodi, CA). Lung tissue sections were examined for the presence of inflammation, cellular infiltrates and epithelial abnormalities in alveolar ducts, blood vessels, and airways.
A semi-quantitative scoring numeric system was used to rank the degree of alveolar, bronchiolar, perivascular, particle-associated, and pleural inflammation in H&E-stained tissue sections as described previously (Silva et al., 2013 (link), 2014 (link)). In brief, an initial blind, qualitative assessment was performed to evaluate the range of inflammatory responses. Subsequently, to minimize observer scoring subjectivity, a scoring rubric was made with categorical definitions extent (focal to diffuse on a scale of 0 to 3), as well as pictorial guidelines of severity, again with ordinal scores (0–3) corresponding to no, minimal, moderate, and marked inflammation, respectively. Blind semi-quantitative histological assessment of all samples was then performed with the product of extent and severity scores to achieve a final histopathological score.

Sun X., Wei H., Young D.E., Bein K.J., Smiley-Jewell S.M., Zhang Q., Fulgar C.C., Castañeda A.R., Pham A.K., Li W, & Pinkerton K.E. (2017). Differential Pulmonary Effects of Wintertime California and China Particulate Matter in Healthy Young Mice. Toxicology letters, 278, 1-8.

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Immunohistochemical Analysis of Adipose Tissue

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Tissues were isolated and placed in formalin. The tissues were then decalcified, paraffin embedded, and sectioned serially at a depth of 2-4 μ. Paraffin was removed using xylene and tissues rehydrated and immunostained, for instance, using anti-Ucp1 (Novus Biologicals, Centennial, CO, NB100-2828, goat polyclonal, 1:100 dilution) and anti-Mrc1 (Abcam, b64693, rabbit polyclonal, 1:200 dilution) antibodies. Secondary antibodies were linked with Alexafluor 488 and 597 dyes. Tissues were counterstained and covered with ProLong Glass Antifade Mountant with NucBlue (Invitrogen, P36985). Stained tissue sections were examined using an Olympus BX41 microscope (Olympus Corporation of the Americas, Waltham, MA) equipped with a reflected fluorescence system or by confocal microscopy (LSM 510 META, Zeiss, Inc., Thornwood, NY, USA) using a 20X/0.75NA objective lens. To ensure signal specificity, controls were performed and the specific absorption spectrum from each primary-secondary pair was captured. To locate the tissue regions, every 5th slide was stained with hematoxylin (Harris Hematoxylin, American Mastertech, Lodi, CA) and eosin (Eosin Y Phyloxine B solution, Electron Microscopy Sciences, Hatfield, PA). Hematoxylin and eosin images were captured by bright-field microscopy using the Olympus BX41 microscope.

Olmsted-Davis E., Mejia J., Salisbury E., Gugala Z, & Davis A.R. (2021). A Population of M2 Macrophages Associated With Bone Formation. Frontiers in Immunology, 12, 686769.

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Quantification of Chondrocytes and Osteoblasts

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For quantification and to ensure representative sections, the entire limb was sectioned serially at a depth of 20 μm. To locate the region of HBF, every fifth slide was stained with hematoxylin (Harris Hematoxylin, American Mastertech, Lodi, California) and eosin (Eosin Y Phyloxine B solution, Electron Microscopy Sciences, Hatfield, Pennsylvania). The entire region of HBF was localized and sections within the center of the lesion were selected as representative. Hematoxylin and eosin serial sections were captured by bright field microscopy using the Olympus BX41 scope. For immunohistochemistry, the images were captured using a Cytation 5 (Biotek Instruments, Winooski, Vermont) imaging reader digital confocal microscope that can reconstruct the image, and the Fiji version of Image J was used to calculate the percentage of chondrocytes (Sox9⁺ or aggrecan⁺ cells) or osteoblasts (Sp7⁺ or osteocalcin⁺ cells) that also co‐express GLAST‐TR using the Fiji plugin (Coloc2) for colocalization analysis. Tissues were co‐stained using Dapi. A complete tissue section was counted entirely to gather a single percentage point and a minimum of six sections from different mice were counted to calculate the mean. SE of the mean was used to determine error.

Mejia J., Salisbury E., Sonnet C., Gugala Z., Olmsted‐Davis E.A, & Davis A.R. (2020). A replicating stem‐like cell that contributes to bone morphogenetic protein 2‐induced heterotopic bone formation. Stem Cells Translational Medicine, 10(4), 623-635.

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Histological Processing and Imaging of Embryos

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Embryos were fixed with histology fixative [1.5% glutaraldehyde, 4% formaldehyde, 3% sucrose in 0.1 mM phosphate buffer (PB, pH 7.3)] overnight at 4°C. Fixed embryos were then dehydrated with a graded series of methanol and embedded in JB4 resin (Polysciences, Inc., Warrington, PA, USA). Sections (4 µm) were cut with an RN2255 microtome (Leica Technology) and were stained with Harris hematoxylin and special eosin II (BBC Biochemical, Mount Vernon, WA, USA). Once the sections were mounted in Polymount (Polysciences, Inc.), the stained sections were imaged with a Provis AX-70 microscope (Olympus America, Inc.) equipped with a RETIGA EXi digital camera (QImaging, Surrey, Canada).

Powell R., Bubenshchikova E., Fukuyo Y., Hsu C., Lakiza O., Nomura H., Renfrew E., Garrity D, & Obara T. (2016). Wtip is required for proepicardial organ specification and cardiac left/right asymmetry in zebrafish. Molecular Medicine Reports, 14(3), 2665-2678.

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Histological and Immunohistochemical Analyses of Mouse Skin

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Paraffin or frozen tissue blocks of mouse skin biopsies were prepared using routine methods and sectioned to obtain consecutive levels of thickness, followed by staining with hematoxylin and eosin. For immunohistochemical analyses of tissues derived from mice, the sections were deparaffinized, treated with citrate buffer for antigen retrieval, stained with antibodies, and then incubated with an avidin-biotin-horseradish peroxidase complex (Vectastain Elite ABC kit; Vector Laboratories). Finally, peroxidase activity was visualized using a 3,3′-diaminobenzidine substrate kit (Vector Laboratories). Tissue sections were counterstained with Harris hematoxylin (BBC Biochemical). Immunofluorescence staining was performed by fixing the frozen sections with 4% PFA. The sections were then permeabilized with 0.2% Tween-20 in PBS, blocked in 5% horse serum, stained with primary antibodies, and incubated with goat antirat Alexa 568 and goat antirabbit 488 antibodies (Thermo Fisher Scientific). Nuclei were stained with DAPI. Images were taken with a DP72 digital camera mounted onto a BX51 microscope (Olympus Corp).

Kim S., Lee S.Y., Bae S., Lee J.K., Hwang K., Go H, & Lee C.W. (2020). Pellino1 promotes chronic inflammatory skin disease via keratinocyte hyperproliferation and induction of the T helper 17 response. Experimental & Molecular Medicine, 52(9), 1537-1549.

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Immunohistochemical Profiling of Xenograft Tumors

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Collected xenograft tumors were fixed in formaldehyde, embedded in paraffin and sectioned on coated slides. Sections were deparaffinized using xylene and hydrated in graded ethanol series (100%, 95%, 75% and 40%). Antigen was retrieved by microwave in 10 mM sodium citrate pH 6.0. Sections were blocked with 0.3% hydrogen peroxide and 1% BSA in TBS. CD90 and AFP primary antibodies were diluted in 1% BSA in TBS at 1:100 and 1:50 ratio, respectively, and incubated ~16 hours at 4°C. For HRP detection, ImmPRESS polymerized reporter enzyme staining system and DAB peroxidase substrate kit (Vector Laboratories, California, USA) was used for detection. Sections were counter stained using Harris Hematoxylin (BBC Biochemical, London, UK) for the appropriate period. For fluorescence detection, FITC-conjugated CD44 antibody was used and nuclear counter stained with DAPI (Sigma). Areas of similar cell morphology and density were compared.

Jung J.W., Yoon S.M., Kim S., Jeon Y.H., Yoon B.H., Yang S.G., Kim M.K., Choe S, & Kuo M.M. (2016). Bone morphogenetic protein-9 is a potent growth inhibitor of hepatocellular carcinoma and reduces the liver cancer stem cells population. Oncotarget, 7(45), 73754-73768.

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Histological Analysis of Murine Femurs

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For histological analysis, isolated femurs from euthanatized mice were fixed with 0.1 M phosphate buffer containing 4% paraformaldehyde (Sigma-Aldrich) at 4 °C for a 3 days. Then they were decalcified in 14.5% EDTA solution (sigma) for 1 weeks at room temperature. Decalcified tissues were rinsed, dehydrated, and paraffin embedded in accordance with general protocols. Paraffinized specimens were sectioned in 4 μm thickness with a microtome. Histological study was performed with Harris hematoxylin (BBC Biochemical, Vernon, WA, USA) and Eosin Y (BBC Biochemical). Images were obtained by inverted Nikon TS2R-FL microscope (Nikon).

Yoo M., Cho S., Shin S., Kim J.M., Park H.G., Cho S., Hwang Y.K, & Park D.H. (2021). Therapeutic Effect of IL1β Priming Tonsil Derived-Mesenchymal Stem Cells in Osteoporosis. Tissue Engineering and Regenerative Medicine, 18(5), 851-862.

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Histopathological Evaluation of Pancreatitis

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All formalin-fixed, paraffin-embedded tissue blocks were sectioned into 5 μm-thick slices and stained with hematoxylin (Harris Hematoxylin; BBC Biochemical, WA, United States) and eosin (Eosin Y; BBC Biochemical).
When histopathological changes were identified in pancreatic tissue, including neutrophilic and lymphocytic inflammation, pancreatic necrosis, peripancreatic fat necrosis, edema, fibrosis, and atrophy, the sample was classified as having pancreatitis (21 (link)). In all tissue samples classified as having pancreatitis, the type of infiltrated inflammatory cells was recorded, and the semiquantitative histopathological grade was assessed based on the surface area affected by a lesion. When <10%, 10–40, and > 40% of the evaluated section were affected by a lesion, the sample was classified as having mild, moderate, and severe pancreatitis, respectively (21 (link)).
When the histopathological changes were absent or minimal changes were observed, the samples were defined as being normal pancreases.

Lee D., Kim S., Koo Y., Chae Y., Wang J., Kim S., Yun T., Yang M.P., Kang B.T, & Kim H. (2023). Expression of vitamin D receptor, CYP24A1, and CYP27B1 in normal and inflamed canine pancreases. Frontiers in Veterinary Science, 10, 1265203.

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