Western blot was performed using conventional methods on samples prepared by RIPA buffer. For each lane, 20 μg of protein was added. Immunoblots were performed against AQP1 (sc-20810, SCBT, 1:2000) and GAPDH (600004–1-Ig, Proteintech, 1:5000) using an anti-rabbit or anti-mouse HRP-conjugate secondary antibody (rabbit: 115-035-003; mouse: 115-035-071, Jackson ImmunoResearch Laboratories) combined with Luminol (Santa Cruz) substrate for detection.
Sc 20810
Manufactured by SCBT
The Sc-20810 is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of the Sc-20810 is to perform specific laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sc 20810
1
Western Blot Analysis of AQP1 and GAPDH
2
Western Blot Analysis of AQP1 and GAPDH
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