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Phospho akt ser308

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Phospho-Akt (Ser308) is an antibody that detects Akt phosphorylated at serine 308. Akt is a serine/threonine protein kinase that plays a key role in multiple cellular processes such as metabolism, proliferation, cell survival, and angiogenesis.

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Lab products found in correlation

Phospho akt ser473, by Cell Signaling Technology (3 mentions) Total gsk3, by Cell Signaling Technology (2 mentions) Phospho akt2, by Cell Signaling Technology (2 mentions) Phospho gsk3 ser9, by Cell Signaling Technology (2 mentions) Phospho foxo1 3 thr24 32, by Cell Signaling Technology (2 mentions) Phospho glycogen synthase ser641, by Cell Signaling Technology (2 mentions) Total akt1, by Cell Signaling Technology (2 mentions) Total akt2, by Cell Signaling Technology (2 mentions) Total glycogen synthase, by Cell Signaling Technology (2 mentions) Total foxo3, by Cell Signaling Technology (2 mentions) Appl1, by Cell Signaling Technology (2 mentions) Antibody to glut2, by Santa Cruz Biotechnology (2 mentions) Myc tag, by Cell Signaling Technology (2 mentions) Enhanced chemi luminescence (ecl), by Merck Group (2 mentions) Total p70s6k, by Cell Signaling Technology (2 mentions) Phospho p70s6k thr389, by Cell Signaling Technology (2 mentions) Chemidoc xr, by Bio-Rad (2 mentions) Quantity one 1 d analysis software, by Bio-Rad (2 mentions) Af1179, by R&D Systems (1 mentions) Ab108341, by Abcam (1 mentions)

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Phospho-Akt (892 citations)

5 protocols using phospho akt ser308

1

Myocardial Oxidative Damage Evaluation

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To evaluate the level of oxidative/nitrosative damage to myocardial proteins samples of the left ventricle (LV) free wall were fixed, embedded in paraplast, sectioned and assessed for 3-nitrotyrosine (3-NTY) residue using an immunofluorescence technique we described previously [34 (link), 35 (link)]. LV tissue sections were also evaluated by immunofluorescence for wheat germ agglutinin (WGA, 1:50, #W11261, Invitrogen), AGE (1:100, #23722, Abcam), RAGE (1:50, #AF1179, R&D), SGK1 (1:100, #32374, Abcam) and ENaC (1:200, #77385, Abcam) expression. Average grey scale intensity was quantified within fixed region of interest rectangles as previously described [35 (link)].
Preparation of LV homogenates, electrophoresis and immunoblotting were described previously [34 (link)]. The following antibodies were used: phospho-Akt Ser308 (1:1000; #4056, Cell Signaling Technology, Inc or CST), phospho-Akt Ser473 (1:1000; #9271, CST), Akt (1:1000; #9272, CST), eNaC (1:1000; #77385, Abcam), RAGE (1:1000; #AF1179, R&D), Phospho-ERK T202/204 (1:1000; #9106, CST), ERK (1:1000; #4695, CST) and pan-actin (1:1000; #4968, CST).
Habibi J., Aroor A.R., Sowers J.R., Jia G., Hayden M.R., Garro M., Barron B., Mayoux E., Rector R.S., Whaley-Connell A, & DeMarco V.G. (2017). Sodium glucose transporter 2 (SGLT2) inhibition with empagliflozin improves cardiac diastolic function in a female rodent model of diabetes. Cardiovascular Diabetology, 16, 9.
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2

Western Blot Analysis of Liver Proteins

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Proteins from total liver or cellular lysates or immunoprecipitated were separated by SDS-PAGE, and probed with different primary antibodies as specified in each figure legend. The specific signals were amplified by addition of horseradish peroxidase-conjugated secondary antibodies and visualized with enhanced chemiluminescence (ECL from Millipore, MA, USA). Western blotting images were processed using a ChemiDoc XRS digital imaging system with Quantity One 1-D analysis software (Bio-Rad Laboratories, Inc., Hercules, CA, USA)
Antibodies from Cell Signaling Technology (working dilution 1:1000) were the following: phospho-Akt (Ser473) (#4060), phospho-Akt (Ser308) (#13038), phospho-Akt2 (#8599) phospho-GSK3 (Ser9)(#9327), phospho-FoxO1-3 (Thr24/32) (#9464), phospho-p70S6K (Thr389) (#9234), phospho-Glycogen Synthase (Ser641) (#3891), total Akt1 (#2967), total Akt2 (#3063, #5239), total Glycogen Synthase (#3893), total FoxO3 (#12829), total GSK3 (#12456), total p70S6K (#2708), APPL1(#3858), Rab5(#2143) (#3547), Rab7(#2094), Myc-Tag (#2272). Antibody to Glut2 (working dilution 1:1000) was from Santa Cruz biotechnology Inc (sc-9117). Original gel images are shown in Supplementary Fig. 9.
Braccini L., Ciraolo E., Campa C.C., Perino A., Longo D.L., Tibolla G., Pregnolato M., Cao Y., Tassone B., Damilano F., Laffargue M., Calautti E., Falasca M., Norata G.D., Backer J.M, & Hirsch E. (2015). PI3K-C2γ is a Rab5 effector selectively controlling endosomal Akt2 activation downstream of insulin signalling. Nature Communications, 6, 7400.
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3

Comprehensive Protein Profiling Protocol

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The following antibodies were used for Western blotting and ChIP: AR (Abcam; ab108341; 1:1,000 for Western blotting), beta-actin (Abcam; ab49900; 1:50,000 for Western blotting), ERG (Abcam; ab92513; 1:1,000 for Western blotting), histone H3 (acetyl K27) (Abcam; ab4729), IRS-1 (Cell Signaling; 2390S; 1:1,000 for Western blotting), IRS-2 (Cell Signaling; 4502S; 1:1,000 for Western blotting), phospho-AKT (Ser308) (Cell Signaling; 13038S; 1:1,000 for Western blotting), phospho-AKT (Ser473) (Cell Signaling; 4060L; 1:1,000 for Western blotting), PTEN (Cell Signaling; 9559L; 1:1,000 for Western blotting), phospho-IGF1R (Cell Signaling; 3024S; 1:1,000 for Western blotting), FKBP5(Cell Signaling; 12210S; 1:1,000 for Western blotting), phospho-Pras40 (Cell Signaling; 2997s; 1:1,000 for Western blotting), phospho-EGFR (Cell Signaling; 3777S; 1:1,000 for Western blotting), phosphor-GSK3B (Cell Signaling; 9336S; 1:1,000 for Western blotting).
Mao N., Gao D., Hu W., Gadal S., Hieronymus H., Wang S., Lee Y.S., Sullivan P., Zhang Z., Choi D., Rosen N., Sawyers C.L., Gopalan A., Chen Y, & Carver B.S. (2020). Oncogenic ERG represses PI3K signaling through down-regulation of IRS2. Cancer research, 80(7), 1428-1437.
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4

Western Blot Analysis of Cardiac Proteins

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Heart lysates of rats were prepared in lysis buffer (20 mM Tris, 150 mM NaCl, 10% glycerol, 20 mM glycerophosphate, 1% NP40, 5 mM EDTA, 0.5 mM EGTA, 1 mM Na3VO4, 0.5 mM PMSF, 1 mM benzamidine, 1 mM DTT, 50 mM NaF, 4 μM leupeptin, pH = 8.0). Samples were resolved by 10% SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk in TBST (50 mM Tris, 150 mM NaCl, 0.5 mM Tween-20, pH = 7.5), and then incubated with primary antibodies overnight. Antibodies used in this study were purchased from Cell Signaling Technology (CST; Danvers, MA, USA), Bioworld: total Akt (CST #4691), phospho-Akt (Ser308) (CST #4060), phospho-Akt (Thr473) (CST #13038), phospho-GSK3β (Ser9) (CST #5558), LC3A/B (CST #12741), PRAS40 (CST #2691), phospho-PRAS40 (Thr246) (CST #13175), PTEN (CST #9188), phospho-PTEN (Ser380) (CST #9551), Bax (CST2772s), Bcl2 (CST3498s), GAPDH (#AP0063), anti-rabbit IgG, (HRP-linked antibody) (CST #7074). Image J software (NIH) was used to perform densitometric analysis (http://rsb.info.nih.gov/ij/).
Cao Y., Song J., Shen S., Fu H., Li X., Xu Y., Wang A., Li X, & Zhang M. (2017). Trimedazidine alleviates pulmonary artery banding-induced acute right heart dysfunction and activates PRAS40 in rats. Oncotarget, 8(54), 92064-92078.
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5

Western Blot Immunodetection Protocol

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Proteins from total liver or cellular lysates or immunoprecipitated were separated by SDS– polyacrylamide gel electrophoresis (SDS–PAGE), and probed with different primary antibodies as specified in each figure legend. The specific signals were amplified by addition of horseradish peroxidase-conjugated secondary antibodies and visualized with enhanced chemiluminescence (ECL from Millipore). Western blotting images were processed using a ChemiDoc XRS digital imaging system with Quantity One 1-D analysis software (Bio-Rad Laboratories, Inc.).
Antibodies from Cell Signaling Technology (working dilution 1:1,000) were the following: phospho-Akt (Ser473) (#4060), phospho-Akt (Ser308) (#13038), phospho-Akt2 (#8599) phospho-GSK3 (Ser9)(#9327), phospho-FoxO1-3 (Thr24/32) (#9464), phospho-p70S6K (Thr389) (#9234), phospho-Glycogen Synthase (Ser641) (#3891), total Akt1 (#2967), total Akt2 (#3063, #5239), total Glycogen Synthase (#3893), total FoxO3 (#12829), total GSK3 (#12456), total p70S6K (#2708), APPL1(#3858), Rab5(#2143) (#3547), Rab7(#2094), Myc-Tag (#2272). Antibody to Glut2 (working dilution 1:1,000) was from Santa Cruz Biotechnology Inc (sc-9,117). Original gel images are shown in Supplementary Fig. 9.
Braccini L., Ciraolo E., Campa C.C., Perino A., Longo D.L., Tibolla G., Pregnolato M., Cao Y., Tassone B., Damilano F., Laffargue M., Calautti E., Falasca M., Norata G.D., Backer J.M, & Hirsch E. (2015). PI3K-C2γ is a Rab5 effector selectively controlling endosomal Akt2 activation downstream of insulin signalling. Nature Communications, 6, 7400.
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