Polygon dmd device

Manufactured by Mightex

The Polygon DMD device is a digital micromirror device (DMD) that can generate customizable light patterns. It consists of an array of microscopic tilting mirrors that can be individually controlled to reflect light in specific directions. The device's core function is to dynamically manipulate light in a programmed manner.

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Lab products found in correlation

0.3 na objective, by Olympus (3 mentions) 1.0na objective, by Olympus (1 mentions)

4 protocols using polygon dmd device

Optogenetic Stimulation of Presynaptic Neurons

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Channelrhodopsin-2 (ChR2) was expressed in presynaptic neurons and activated with a brief light pulse from a blue LED (473 nm) (Thorlabs). For wide-field illumination, light was delivered via a 10 × 0.3 NA objective (Olympus) centered on the recorded cell. LED power was routinely calibrated at the back aperture of the objective. LED power and duration were adjusted to obtain reliable responses, with typical values of 0.4 to 10 mW and 2 ms, respectively. Subcellular targeting experiments were performed with a Polygon DMD device (Mightex) focused through a 10 × 0.3 NA objective (Olympus) with a 75 μm pixel size. Pulses were delivered at 1 Hz using a pseudo-random 10 × 10 grid pattern, yielding an effective mapping area of 750 μm × 750 μm. Experiments used a 2 ms LED pulse yielding an effective power of 0.17 mW per pixel.

Liu X., Dimidschstein J., Fishell G, & Carter A.G. (2020). Hippocampal inputs engage CCK+ interneurons to mediate endocannabinoid-modulated feed-forward inhibition in the prefrontal cortex. eLife, 9, e55267.

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Selective Optogenetic Manipulation of pBLA Terminals in dBNST Subregions

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To selectively activate the pBLA axonal terminals in either adBNST or ovBNST, we delivered a focalized light spot (~120 µm in diameter) generated by a Polygon DMD device (Mightex) to either of the two dBNST subregions at a random order. Their effects were then examined on the firing or synaptic responses of the adBNST cells.

Han R.W., Zhang Z.Y., Jiao C., Hu Z.Y, & Pan B.X. (2024). Synergism between two BLA-to-BNST pathways for appropriate expression of anxiety-like behaviors in male mice. Nature Communications, 15, 3455.

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Optogenetic Control of Glutamate Release

Cited 2 times

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Glutamate release was triggered by activating channelrhodopsin-2 (ChR2) present in presynaptic terminals of either thalamic inputs to the PFC, or local circuit interneurons as previously described (Anastasiades et al., 2018a (link); Little and Carter, 2012 (link)). ChR2 was activated with 1–8 ms pulses of 473 nm light from a blue light-emitting diode (LED; 473 nm; Thorlabs) through a 10X 0.3 NA objective (Olympus) with a power range of 0.1–20 mW. For widefield recordings in the PFC, the objective was always centered 200 μm from the pial surface of the cortex. For focused optogenetic stimulation over the apical or basal dendrites blue (473 nm) LED light was focused through a 60X 1.0NA objective (Olympus). For ArchT mediated suppression of activity this was interleaved with light from a yellow (590 nm) light focused through the same 60X 1.0NA objective. Subcellular targeting (sCRACM) experiments were performed using a Polygon DMD device (Mightex) focused through a 10X 0.3 NA objective (Olympus) with a 75 μm pixel size. Pulses were delivered at 1 Hz using a pseudo-random 10 × 10 grid pattern, yielding an effective mapping area of 750 μm × 750 μm. Experiments used a 4 ms LED pulse yielding an effective power of 0.17 mW per pixel.

Anastasiades P.G., Collins D.P, & Carter A.G. (2020). Mediodorsal and ventromedial thalamus engage distinct L1 circuits in the prefrontal cortex. Neuron, 109(2), 314-330.e4.

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Spatially Restricted Optogenetic Mapping

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To map the outputs of st-ChroME+ interneurons, stimulation parameters were developed to produce robust, spatially restricted AP firing of these cells. Expression time was tightly controlled (14–16 days post injection) to ensure reliable, yet spatially restricted, firing across the presynaptic population. Calibration recordings were performed in cell-attached mode to avoid perturbing the intracellular environment of the cell. A blue (473 nm) LED was passed through a Polygon DMD device (Mightex) and focused through a 10X 0.3 NA objective (Olympus) with pixel size calibrated to 75 μm. Pulses were delivered at 1 Hz using a pseudo-random 10 × 10 grid pattern, yielding an effective mapping area of 750 μm × 750 μm. Experiments used a 1 ms LED pulse yielding an effective power of 0.02 mW per pixel. Presynaptic APs occurred within 30 ms of LED onset and a postsynaptic IPSCs detection window was set to include responses 100 ms after LED onset to calculate the IPSC peak amplitude per pixel, as described previously (Anastasiades et al., 2018b (link)).

Anastasiades P.G., Collins D.P, & Carter A.G. (2020). Mediodorsal and ventromedial thalamus engage distinct L1 circuits in the prefrontal cortex. Neuron, 109(2), 314-330.e4.

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