Chicken core histones

C. albicans overnight cultures were diluted to an OD₆₀₀ of 0.25 in YPD medium and CPTH2 was added at the indicated concentrations. Cells were incubated at 30 °C for 4 h prior to OD₆₀₀ measurement and harvesting. Whole-cell extract preparation and immunoblotting was performed as described previously [20 (link)]. Histone acetylation was detected using an anti-acetyl lysine antibody (NEB, Ipswich, MA, USA), and an anti-PSTAIRE (recognizing Cdc28) antibody (Santa Cruz Biotechnology, Dallas, TX, USA) was used as the loading control. Chicken core histones (1 µg, Millipore, Burlington, MA, USA) were used as positive controls and recombinant histone H3 and H4 (NEB, Ipswich, MA, USA) were used as negative controls (0.1 µg each per lane).

S3 Table in S1 File contains detailed information about the sources of the recombinant enzymes. Peptide substrates were from AnaSpec (Fremont, CA). α-Ketoglutarate (disodium salt dihydrate, 75892), L-ascorbic acid (A0278), ammonium iron (II) sulfate hexahydrate (215406), BSA (A3803), and β-nicotinamide adenine dinucleotide (NAD, N6522) were from Sigma-Aldrich. Tween-20 (10%, 28320) was from Thermo Fisher Scientific. 384 Alpha-plates, acceptor beads (5 mg/mL), alpha streptavidin donor beads (5 mg/mL, 6760002), and 5× Epigenetic buffer (AL008) were from PerkinElmer (Waltham, MA). Chicken core histones were obtained from Millipore (Billerica, MA). HeLa cell nucleosomes were from Reaction Biology Corporation (Malvern, PA). Nucleosome antigen was from Arotec Diagnosis (ATN02, Wellington, New Zealand). Small molecule inhibitors used as positive controls included S2101 (CalBiochem/Millipore; 489477), 2,4-Pyridinedicarboxylic acid (AK Scientific, Union City, CA; 00473), 8-hydroxyquinoline (Sigma-Aldrich; S018), 8-hydroxy-5-quinolinecarboxylic acid (Sigma-Aldrich; SML0057), S-(5′-Adenosyl)-L-homocysteine (Sigma-Aldrich; A9384), Trichostatin A (Sigma-Aldrich; T8552), and EX-527 (Sigma-Aldrich; E7024).