Three syringe pumps (11 Elite C300918, Harvard Apparatus, U.S.A.) were connected to the fabricated device through Tygon tubes (Saint-Gobain, Courbevoie, France). For one core inlet, a mixture of 3 mg/mL collagen, 2 × 106 cells/mL HUVECs, and 0.1 M CaCl2 (Daejung Chemicals, Republic of Korea) was supplied to culture into a blood vessel. For another core inlet, 0.1 M CaCl2 was injected to formulate a hollow channel inside the scaffold. For the outer layer inlet, a 2% w/v mixture of gelatin (Sigma-Aldrich, U.S.A.) and alginate (Daejung Chemicals, Republic of Korea) (70 vs. 30 ratio) was supplied as the body of the scaffold. The extruded scaffold was submerged into a 0.1 M CaCl2 bath through the outlet and then cross-linked. Calcium ions of the CaCl2 cross-linked with sodium alginate into calcium alginate so that no hydrogel in the hollow channel remained. The gelatin scaffold was washed with phosphate-buffered saline (PBS, Sigma-Aldrich, U.S.A.). The washed scaffold was cultured in an incubator at 37°C with 5% CO2 and replaced with a fresh medium every 2 days.
Cacl2
Manufactured by Daejung Chemicals
CaCl2 is an inorganic compound commonly known as calcium chloride. It is a white, crystalline solid that is highly soluble in water. The primary function of CaCl2 is to serve as a desiccant, absorbing moisture from the surrounding environment.
Automatically generated - may contain errors
Lab products found in correlation
Potassium chloride (kcl), by Daejung Chemicals (2 mentions)
Gelatin, by Merck Group (1 mentions)
Acetic acid solution, by Merck Group (1 mentions)
Zno np, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Escherichia coli atcc 25922, by American Type Culture Collection (1 mentions)
Bacillus cereus atcc 11 778, by American Type Culture Collection (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Glycerol, by Daejung Chemicals (1 mentions)
Sulfuric acid, by Daejung Chemicals (1 mentions)
Acetic acid, by Daejung Chemicals (1 mentions)
Tert butanol, by Daejung Chemicals (1 mentions)
Nc membrane, by Cytiva (1 mentions)
Tween 20, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Sucrose, by Daejung Chemicals (1 mentions)
Sodium chloride (nacl), by Daejung Chemicals (1 mentions)
Mgcl2, by Daejung Chemicals (1 mentions)
Sodium carbonate, by Daejung Chemicals (1 mentions)
Osthole, by Merck Group (1 mentions)
7 protocols using cacl2
1
Fabrication of Vascularized Tissue Scaffold
2
Antimicrobial Activity of Chitosan-ZnONP Composite
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The WsKG used in this study was 6-year-old root grown in Sansam Village (Wanju, Korea). Chitosan (75–85% deacetylated), acetic acid solution, and ZnONP (average particle size of 20 ± 5 nm) were purchased from Sigma-Aldrich (Seoul, Korea). K2SO4 and CaCl2 were purchased from Daejung chemicals & Metals Co. (Siheung, Korea). Escherichia coli ATCC 25,922 and Bacillus cereus ATCC 11,778 were brought from the American Type Culture Collection (ATCC, Seoul, Korea). E. coli and B. cereus were cultivated at 37 °C for 16 h by picking one inoculation loop and spreading on Luria–Bertani (LB) medium (BD DIFCO, New Jersey, USA). Microbial stock was prepared by inoculating LB broth and 50% glycerol (6:4 v/v) with the bacteria, which was stored at −80 °C.
3
Alginate-based Biomaterial Synthesis
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Sodium alginate was supplied from Sigma-Aldrich, USA. CaCl 2 and glycerol were purchased from Daejung, South Korea. Trehalose as a dihydrate form was derived from Hayashibara, Japan. Deionized water obtained from distillation using a water purification system (Pacific TII 12 UV, Thermo Scientific, Hungary).
4
Preparation of Korean Red Pine Wood Powder
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Korean red pine (Pinus densiflora S. et Z.) was obtained from the Experimental Forest of Kangwon National University (Chuncheon, Korea). The degreased wood powder was prepared using an ethanol/benzene (1/2, v/v) solution in a Soxhlet extractor operating at 90 °C for 6 h. AL, CaCl2, ChCl, LA, sodium chlorite, acetic acid, 50% NaOH solution, tert-butanol, and sulfuric acid were purchased from Daejung Chemical & Metals Co., Ltd. (Siheung, Korea) and used without further purification. Commercial PCNFs were supplied by Cellulose Lab, Co., Ltd. (Fredericton, NB, Canada).
5
Oligonucleotide-based Assay Design
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The oligonucleotides used in this study (Table S1 ) were purchased from Integrated DNA Technologies (Skokie, IL, USA) and Bionics (Seoul, Korea); HAuCl₄, Tween 20, and sodium azide from Sigma-Aldrich (St. Louis, MO, USA); sodium carbonate, sucrose, NaCl, MgCl2, KCl, and CaCl2 from Daejung Chemicals & Metals (Gyeonggi-do, Korea); bovine serum albumin (BSA) and PCR premix (RT500S) from Enzynomics (Daejeon, Korea); streptavidin from Biolegend (CA, USA); backing card and absorbent pads from TWOHANDS (Gyeonggi-do, Korea); and NC membrane from Whatman (Maidstone, UK).
6
Vascular Smooth Muscle Contractility Assay
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BaCl2, glucose, magnesium sulfate (MgSO4), potassium phosphate monobasic (KH2PO4), KCl, sodium chloride (NaCl), sodium hydrogen carbonate (NaHCO3), CaCl2, and urethane were sourced from Daejung Chemicals & Metals Co., Ltd. (Siheung, Republic of Korea). Dimethyl sulfoxide (DMSO) was purchased from Junsei (Tokyo, Japan). Phenylephrine, acetylcholine (Ach), and ethylene glycol-bis(2-aminoethylether)-N,N‚N′,N′-tetraacetic acid (EGTA), Ang II, SK&F96365, nifedipine, imperatorin (>98.0% purity verified by HPLC, CAS: 482-44-0), and osthole (≥95.0% purity verified by HPLC, CAS: 484-12-8) were purchased from Sigma Aldrich, Inc. (St. Louis, MO, USA). 4-AP, Glib, and TEA were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
7
Synthesis of Hemostatic Carboxymethyl Starch
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Carboxymethyl starch (50 wt%; JRS PHARMA GmbH, Germany) was dissolved and stirred in a solution of calcium chloride (0.08 wt%; CaCl2, Daejung, Siheung, Korea) in ethanol (99%, Daejung, Siheung, Korea) for 2 h. The obtained solution containing precipitate was filtered using a filter paper to eliminate unreacted calcium chloride, which was then rinsed with ethanol three times. Subsequently, the filtered solution was dried in an oven (40 °C for 24 h) and pulverized by cryogenic grinding. The ground hemostatic particles were then sieved using a sieve tray (nominal aperture 125 μm, Daihan Scientific, Seoul, Korea) to obtain a homogeneous powder. The powdered sample was sterilized under UV light (254 nm) and kept in a sealed pack for further use.
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