Bxpc 3

Human pancreatic cancer cell lines BxPC3 and Panc-1 were purchased from the American Type Culture Collection (ATCC; LGC Promochem, Borås, Sweden). Both cell lines overexpress EpCAM [62 (link),63 (link)] and the BxPC3 cell line overexpresses HER3 [64 (link),65 (link)]. BxPC3 cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (L0500, Biowest) supplemented with 2 mM L-glutamine, 10% fetal bovine serum (FBS; F7524, Sigma-Aldrich), and 1% penicillin–streptomycin (L0022, Biowest, Berlin, Germany). Panc-1 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; 11594486, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 2 mM L-glutamine, 10% FBS, and 1% penicillin–streptomycin solution. Cells were cultured at 37 °C and 5% CO₂ atmosphere unless stated otherwise. Trypsin–ethylenediaminetetraacetic acid (EDTA) solution (25200056, Thermo Fisher Scientific, Waltham, MA, USA) was used for cell detachment.

BxPC-3 and Capan-1 cell lines were purchased from the ATCC (Manassas, VA, USA). MIA PaCa-2, MCF7, SK-BR-3 and MDA-MB-231 cell lines were in possession of 4Cell Therapies S.A. and were authenticated by Eurofins Genomics (Ebersberg, Germany). Capan-1 cells were cultured in IMDM, 20% FBS and 1× pen/strep (Biowest) on collagen I (Merck, Darmstadt, Germany)-coated plates. The BxPC-3 cell line was cultured with RPMI 1640 with 10% FBS and 1 × pen/strep (Biowest). The SK-BR-3 cell line was cultured in McCoy’s 5a supplemented with 20% FBS and 1 × pen/strep (Biowest). All other cell lines were cultured in DMEM high glucose supplemented with 10% FBS and 1 × pen/strep (Biowest).

PDAC cell lines BxPC-3, Capan-1, MIA PaCa-2, PANC-1, and AsPC-1 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Cell lines were cultured in Roswell Park Memorial Institute medium 1640 (RPMI) (used for Capan-1, BxPC-3, and AsPC-1) or Dulbecco’s Modified Eagle’s Medium (DMEM) (used for MIA PaCa-2, and PANC-1), supplemented with 10% fetal bovine serum (FBS; Biowest, Riverside, MO, USA) and 1% antibiotic–antimycotic reagent (Gibco, Waltham, MA, USA) at 37 °C and 5% CO₂. Specifically, Capan-1 cells were cultured in a 10 cm dish in the RPMI medium to 90% confluency before subculture every seven days. Medium was renewed every three days.