Distinct PBMC populations were enumerated by flow cytometry, as described (33 (link), 50 (link)). Phenotypic analyses were performed using Abs directed against CD3 (clone SP34-2), CD4 (L200), CD8 (RPA-T8), CD14 (M5E2), CD20 (2H2), CD45RA (5H9), CD56 (MY31) and CD127 (HIL-7R-M21) from BD Pharmingen (Franklin Lakes, NJ). Anti-CD159a (NKG2A) (Z199) was from Beckman Coulter and anti-CD28 (CD28.2) from Biolegend (San Diego, CA). Anti-CD25 (BC96) was from eBioscience (San Diego, CA) and anti-CD62L (SK11) from Becton Dickinson (Franklin Lakes, NJ). Data were acquired on a LSR II or LSR Fortessa (BD Bioscience) and analyzed with FlowJo software.
Cd127 hil 7r m21
Manufactured by BD
Sourced in United States
The CD127 (HIL-7R-M21) is a laboratory equipment product. It is a cell surface marker that serves as a receptor for the cytokine interleukin-7 (IL-7). The CD127 molecule is expressed on a variety of immune cells, including T cells, B cells, and natural killer cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd25 bc96, by Thermo Fisher Scientific (1 mentions)
Lsrfortessa, by BD (1 mentions)
Cd45 2d1, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Facsaria, by BD (1 mentions)
Igd ia6 2, by BD (1 mentions)
Cd27 m t271, by BioLegend (1 mentions)
Rpa t4, by BD (1 mentions)
Facsaria fusion, by BD (1 mentions)
Ficoll paque plus, by GE Healthcare (1 mentions)
4 protocols using cd127 hil 7r m21
1
Multiparametric Flow Cytometry for PBMC Analysis
2
Comprehensive T cell Immunophenotyping
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T cells were labeled with monoclonal antibodies against CD4 (OKT4), CD25 (BC96), CD38 (HIT1), CD48 (TU145), CD52 (4C8), CD127 (HIL-7R-M21), CD161 (HP-3G10), CD196 (11A9), and CD226 (DX11), all from BD or Biolegend, as described previously (12 (link)). Intracellular staining for HIF-1a (241812 from R&D systems) and AhR (T49-550 from BD) was done after fixation and permeabilization using kit from R&D Systems. The profiling was done after 7 days in culture if not mentioned otherwise in the figure legend. The data acquisition was done at LSRFortessa (BD biosciences), and analysis was performed with FlowJo v10 software (Tree star).
3
Multi-Parameter Flow Cytometry Immunophenotyping
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The following anti-human mAb were used to stain cell suspension for FACS analysis: CD3 (SK7), CD4 (SK3), CD34 (581), CD8 (SK1), CD19 (HIB19), CD10 (HI10a), CD38 (HB7), NKp46 (9E2), CD16 (3G8), CD161 (DX12), CD56 (B159), HLA-DR (L243), CD244.2 (2B4), HLA-A/B/C (G46-2.6), CD117 (YB5.B8), CD14 (M5E2), CD122 (Mik-3), TCR-β (T10B9.1A-31), CD127 (hIL-7R-M21), CD11c (B-ly6), CD7 (M-T701), CD45 (2D1) from (BD Bioscience, CA, USA). All washings and reagent dilutions were done with phosphate buffered saline (PBS) containing 2% foetal calf serum (FCS). All acquisitions were performed using FACSVerse cytometers and cell sorting was perform using FACS ARIA, all machines were interfaced to the FACS-Diva software (BD Bioscience). Data analysis was performed using FlowJo software from TreeStar Inc. (Ashland, OR, USA).
4
Isolation and Sorting of CD8+ T Cell Subsets
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