13c3 testosterone

Manufactured by Cerilliant

13C3-testosterone is a stable isotope-labeled internal standard used for the quantitative analysis of testosterone in biological samples. It serves as a reference compound for mass spectrometry-based analytical methods.

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Lab products found in correlation

Chromolith reverse phase column rp 18 endcapped 100 2mm, by Merck Group (1 mentions) Jetstream source, by Agilent Technologies (1 mentions) Tert butyl methyl ether, by Merck Group (1 mentions) Prism version 8, by GraphPad (1 mentions) Hydroxylamine hydrochloride, by Merck Group (1 mentions) Masshunter software, by Agilent Technologies (1 mentions) Masshunter workstation software, by Agilent Technologies (1 mentions) Estrone, by Merck Group (1 mentions) Estriol, by Merck Group (1 mentions) Progesterone, by Merck Group (1 mentions) 17β estradiol, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Testosterone, by Merck Group (1 mentions) Isopropyl alcohol, by Merck Group (1 mentions)

2 protocols using 13c3 testosterone

Quantifying Testosterone by UHPLC-MS/MS

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Testosterone quantification was determined using Agilent’s UHPLC Infinity II and 6495 triple quadrupole mass spectrometer, and MassHunter workstation software (8.0.8.23.5). Briefly, plasma samples containing 0.5 ng/mL ¹³C₃-testosterone (Cerilliant, Round Rock, TX) were extracted with tert-butyl methyl ether (Sigma 34875), dried, and derivatized using hydroxylamine hydrochloride (Sigma 431362). The recovered ketoxime steroids were reconstituted in methanol/water (1:1 v/v) and injected into the Infinity II UHPLC. Ketoxime steroids were separated using a Chromolith reverse phase column (RP-18 endcapped 100–2mm, Sigma 152006) and introduced into a JetStream source (Agilent) for triple quadrupole analysis. Data were analyzed and quantified using MassHunter software (Agilent) and GraphPad PRISM (version 8.0) software was used to graph and perform statistics (two-sided Students t-test)^{61 (link),62 (link)}.

Vellano C.P., White M.G., Andrews M.C., Chelvanambi M., Witt R.G., Daniele J.R., Titus M., McQuade J.L., Conforti F., Burton E.M., Lastrapes M.J., Ologun G., Cogdill A.P., Morad G., Prieto P., Lazar A.J., Chu Y., Han G., Khan M.A., Helmink B., Davies M.A., Amaria R.N., Kovacs J.J., Woodman S.E., Patel S., Hwu P., Peoples M., Lee J.E., Cooper Z.A., Zhu H., Gao G., Banerjee H., Lau M., Gershenwald J.E., Lucci A., Keung E.Z., Ross M.I., Pala L., Pagan E., Segura R.L., Liu Q., Borthwick M.S., Lau E., Yates M.S., Westin S.N., Wani K., Tetzlaff M.T., Haydu L.E., Mahendra M., Ma X., Logothetis C., Kulstad Z., Johnson S., Hudgens C.W., Feng N., Federico L., Long G.V., Futreal P.A., Arur S., Tawbi H.A., Moran A.E., Wang L., Heffernan T.P., Marszalek J.R, & Wargo J.A. (2022). Androgen receptor blockade promotes response to BRAF/MEK-targeted therapy. Nature, 606(7915), 797-803.

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Steroid Quantification Using Isotope Dilution

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Estrone, 17-β-estradiol, estriol, progesterone, testosterone, methanol, acetonitrile, isopropyl alcohol, and sodium hydroxide were of the highest grade commercially available from Sigma-Aldrich (St. Louis, MO). The following stable isotopes were purchased from Cambridge Isotope Laboratories (Andover, MA): [D₄]-estradiol (2,4,16,16, 95–97 atom% [D₄]), [D₄]-estriol (2,4,16,17, 98 atom% [D₄]), [D₄]-Estrone (2,4,16,16, 97 atom% [D₄]), and [D₉]-progesterone (2,2,4,6,6,17A,21,21,21, 98 atom% [D₉]). [¹³C₃]-testosterone (2, 3, 4 99 atom% [¹³C₃]) was purchased from Cerilliant (Round Rock, TX). Stable isotope structures listed in Supplementary Table 1.

Wooding K.M., Hankin J.A., Johnson C.A., Chosich J.D., Baek S.W., Bradford A.P., Murphy R.C, & Santoro N. (2015). Measurement of estradiol, estrone, and testosterone in postmenopausal human serum by isotope dilution liquid chromatography tandem mass spectrometry without derivatization. Steroids, 96, 89-94.

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