Bx63 compound microscope

Manufactured by Olympus

The BX63 is a compound microscope designed for advanced microscopy applications. It features a sturdy, ergonomic design and high-quality optics to provide clear, detailed images. The core function of the BX63 is to magnify and observe small specimens for research, educational, or industrial purposes.

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K850 critical point dryer, by Quorum Technologies (1 mentions) K550x sputter coater, by Quorum Technologies (1 mentions) Item software, by Olympus (1 mentions)

4 protocols using bx63 compound microscope

Larval Morphology and Measurements

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Measurements (mm) of the first (L1) and second (L2—mature) larval instars were made with cellSens Dimension v1.9 software using an Olympus BX63 compound microscope. Photographs showing the overall habitus of the larvae (L2) and the male adult used for rearing were taken with an Olympus DP72 digital camera mounted on an Olympus SZX16 compound microscope.
To prepare the microscope slides for the morphological analyses, the preserved larvae were soaked in 10% KOH for several days, rinsed in distilled water, then immersed in lactic acid. Photographs showing the various details of the external structure of the larvae and male aedeagus were taken with an Olympus DP21 digital camera mounted on an Olympus BX63 compound microscope or with a VEGA3 TESCAN SEM and subsequently corrected using CorelDRAW Graphics Suite X6. The style and terminology of the morphological description are according to Staniec et al. [6 ,7 (link)].
The voucher specimens are deposited in the collection of the Department of Zoology and Nature Protection, Institute of Biological Sciences, Marie Curie-Sklodowska University, Lublin. The material used for the morphological examination and measurements (15 larvae—8 L1 and 7 L2) is listed in Table 2.

Staniec B., Pietrykowska-Tudruj E., Wagner G.K., Mazur A, & Kowalczyk M. (2022). Synthesis of Current Knowledge of the Morphology of the Larval Stages of Paederinae (Coleoptera; Staphylinidae), with a First Insight into the Mature Larva of Pseudomedon Mulsant & Rey, 1878, in the Light of a New Systematic Division. Insects, 13(11), 982.

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Ant Nest Microstructure Analysis

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The microstructure of the ant nests was examined and recorded using an Olympus DP21 digital camera mounted on an Olympus BX63 compound microscope or VEGA3 TESCAN SEM (Figure 1B–J). Fresh samples in the field were recorded using Panasonic DMC-TZ60 digital camera (Figure 1A1). For the SEM techniques, samples were dried at the critical point of CO2 using an Emitech K850 Critical Point Dryer. The dried samples of carton were coated with a layer of gold using an Emitech K550X Sputter Coater. Thereafter, the samples were placed directly in the SEM chamber for examination.

Wagner G.K., Jaszek M., Staniec B., Prendecka M., Pigoń D., Belcarz A., Stefaniuk D., Matuszewska A., Pietrykowska-Tudruj E, & Zagaja M. (2021). Lasius fuliginosus Nest Carton as a Source of New Promising Bioactive Extracts with Chemopreventive Potential. International Journal of Molecular Sciences, 22(9), 4392.

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Morphometric Analysis of Larval Beetles

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Measurements of the larvae of both species, made using an Olympus BX63 compound microscope in cellSens Dimension v1.9 software, are given in millimetres, as explained in detail in Pietrykowska-Tudruj and Staniec (2012) . Measurements (Table 1) were made on freshly killed specimens. The terms of morphological structures, chaetotaxy (selected aspects only) and their abbreviations generally follow Ashe and Watrous (1984) and Staniec et al. (2018) (link), with modifications in some of the figures. The material examined for the measurements is listed in Table 1. The material examined for morphological descriptions includes four or five specimens of the late-instar larva of each species. The voucher specimens are deposited in the collections of the Department of Zoology, Maria Curie Skłodowska University, Lublin.

Staniec B., agaja M., Pietrykowska-Tudruj E, & Grzegorz K. W.a.g.n.e.r. (2018). Comparative larval ultramorphology of some myrmecophilous Aleocharinae (Coleoptera, Staphylinidae), with a first description of the larvae of Amidobiatalpa (Heer O, 1841) and Oxypodahaemorrhoa (Mannerheim C.G., 1830), associated with the Formicarufa species group. ZooKeys.

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Characterization of Beef Sarcocysts

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Sarcocysts were isolated from beef loin samples from Argentina. Thick walled sarcocysts (≥ 3 µm) were identified microscopically and fixed in 2.5% glutaraldehyde solution as described previously (Moré et al. 2014) . DNA was extracted from purified individual sarcocysts or cysts portions, amplified and sequenced with primers SarcoFext and SarcoRext as described (Moré et al. 2014) . Epoxy-resin embedded tissues were transported to the Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Republic of South Africa for the present study. Toluidine blue-stained resin sections of the four microcysts were photographed with an Olympus BX63 compound microscope (Olympus, Wirsam, South Africa). Ultrathin resin sections were contrasted with uranyl acetate and lead citrate and examined in a Philips CM10 transmission electron microscope (FEI, Eindhoven, The Netherlands) operated at 80 kV. Digital images were captured with a Megaview III side-mounted digital camera and iTEM software (Olympus Soft Imaging Solutions GmbH, Münster, Germany). Electronic images (TIFF) were sent from South Africa to the senior author (JPD) at Beltsville, Maryland to avoid importation of tissues from cattle in the USA.

Dubey J.P., Moré G., van Wilpe E., Calero-Bernal R., Verma S.K, & Schares G. (2016). Sarcocystis rommeli, n. sp. (Apicomplexa: Sarcocystidae) from Cattle (Bos taurus) and its Differentiation from Sarcocystis hominis. The Journal of eukaryotic microbiology, 63(1).

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