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Sirt2flox flox mice

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SIRT2flox/flox mice are genetically modified mice with Sirt2 gene flanked by loxP sites. The Sirt2 gene encodes the sirtuin 2 protein, which is a member of the sirtuin family of NAD+-dependent deacetylases. These mice can be used to study the biological functions of the Sirt2 protein through conditional gene knockout techniques.

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Lab products found in correlation

Alb cre mice, by Jackson ImmunoResearch (2 mentions)

2 protocols using sirt2flox flox mice

1

Generating Liver-Specific SIRT2 Knockout Mice

Cited 1 time
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The liver-specific SIRT2-KO mice were produced by crossing SIRT2flox/flox mice obtained from Johan Auwerxd Laboratory (Switzerland)47 (link) and Alb-Cre mice purchased from Jackson Laboratory (USA) in a C57BL/6 background. Recombinant AAV8 constructs expressing targeted mouse SIRT2, SIRT2-H187A, LCN2, C/EBPβ, or mutated C/EBPβ (K101R and K102R) under the control of TBG promoter were generated by Sunbio Techservice Inc. (Shanghai, China). A noncoding plasmid carrying only the TBG promoter was used to produce control vector particles. All animals received care in compliance with protocols approved by the Institutional Animal Use and Care Committee of Shanghai Jiaotong University School of Medicine.
Zhang Y., Long X., Ruan X., Wei Q., Zhang L., Wo L., Huang D., Lin L., Wang D., Xia L., Zhao Q., Liu J., Zhao Q, & He M. (2021). SIRT2-mediated deacetylation and deubiquitination of C/EBPβ prevents ethanol-induced liver injury. Cell Discovery, 7, 93.
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2

Generating Liver-Specific SIRT2 Knockout Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The liver-specific SIRT2-KO mice were produced by crossing SIRT2flox/flox mice obtained from Johan Auwerxd Laboratory (Switzerland)47 (link) and Alb-Cre mice purchased from Jackson Laboratory (USA) in a C57BL/6 background. Recombinant AAV8 constructs expressing targeted mouse SIRT2, SIRT2-H187A, LCN2, C/EBPβ, or mutated C/EBPβ (K101R and K102R) under the control of TBG promoter were generated by Sunbio Techservice Inc. (Shanghai, China). A noncoding plasmid carrying only the TBG promoter was used to produce control vector particles. All animals received care in compliance with protocols approved by the Institutional Animal Use and Care Committee of Shanghai Jiaotong University School of Medicine.
Zhang Y., Long X., Ruan X., Wei Q., Zhang L., Wo L., Huang D., Lin L., Wang D., Xia L., Zhao Q., Liu J., Zhao Q, & He M. (2021). SIRT2-mediated deacetylation and deubiquitination of C/EBPβ prevents ethanol-induced liver injury. Cell Discovery, 7, 93.
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