A fasting blood sample of ∼5 mL was collected from each participant in a heparin sodium anticoagulant tube at enrollment. These tubes were centrifuged at 1000 r/min for 5 min and the supernatant was sent within 4 h for lipid measurement of TC, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG) to the clinical laboratory of the Hua County People's Hospital. Lipid measurements were conducted using a HITACHI7600 automatic biochemistry analyzer (Hitachi High Technologies Co., Tokyo, Japan) with commercially available reagents (Autobio Diagnostics Co., Ltd., Beijing, China) for analysis of TC, TG, LDL-C, and HDL-C.
Hitachi7600 automatic biochemistry analyzer
Manufactured by Hitachi
Sourced in Japan
The HITACHI7600 is an automatic biochemistry analyzer designed for clinical laboratory applications. It is capable of performing a variety of biochemical tests and analyses on patient samples. The device automates the process of sample handling, reagent dispensing, and data processing to provide efficient and reliable test results.
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Lab products found in correlation
3 protocols using hitachi7600 automatic biochemistry analyzer
1
Fasting Lipid Measurement Protocol
2
Fasting Lipid Measurement Protocol
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At physical examination, a fasting blood sample of ~ 5 mL was collected from each participant in a heparin sodium anticoagulant tube. These tubes were then centrifuged at 1000 rpm for 5 min and the supernatant was sent for lipid measurement of TC, LDL-C, HDL-C and TG within 4 h in the clinical laboratory of Hua County People’s Hospital. Lipid measurements were conducted using a HITACHI7600 automatic biochemistry analyzer (Hitachi High Technologies Co., Tokyo, Japan) with commercially available reagents (Autobio Diagnostics Co., Ltd., Beijing, China) for analysis of TC, TG, LDL-C and HDL-C.
3
Evaluating Metabolic Biomarkers in Mice
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The mice were humanly sacrificed by cervical dislocation after 8 weeks of experimental duration. By extirpating the eyeball, blood was collected, and serum was separated by centrifuging at 1000g for 10 min. The serum of all experimental groups was subjected to the HITACHI 7600 automatic biochemistry analyzer (Hitachi High-Technologies Co., Tokyo, Japan) to determine the concentration of aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TG), and total cholesterol (TC). A fasting blood glucose (FBG) level was measured by a glucometer via puncturing the tail vein of mice. The serum insulin levels and non-esterified fatty acids (NEFA) were measured by an ELISA kit according to the protocol of the manufacturer.
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