F x ananassa cv. “Camarosa” fruits were cross sectioned and 2 mm thick pie sections containing the epidermis, cortex, and pith were dissected with a scalpel. Samples were fixed in 2,5% glutaraldehyde (Acros organics, Thermo Fisher Scientific) and 50 mM sodium cacodylate buffer (pH 7,4) under vacuum for 2 × 15 min at 4°C and left in fixative o/n. Fixed samples were washed with buffer three times for 10 min and then post- fixed in buffered 1% OsO4 (Electron Microscopy Sciences, 19150) for 60 min. After washing them in water, samples were incubated with 2% uranyl acetate (aqueous) (Electron Microscopy Sciences, 22400) for 2 h and then washed with water. After samples were dehydrated through an ethanol series (Panreac, 141086.1214; 50, 70, and 95% each step 30 min; 100% for 30 min and 100% for 30 min) and incubated in a solution of London Resin White (Electron Microscopy Sciences, 14381-UC) and ethanol (1:1) overnight. Samples were incubated in pure London Resin White for 5 h and London Resin White overnight; polymerization was performed at 65°C for 24 h. Ultrathin sections (50–70 nm) were obtained with an ultramicrotome Leica EM UC7/FC7. Sections were examined with a JEM-1400 (Jeol, Málaga, Spain) transmission electron microscopy.
Em uc7 fc7
Manufactured by Leica
Sourced in Germany
The EM UC7/FC7 is an ultramicrotome designed for preparing ultrathin sections of biological and material samples for transmission electron microscopy (TEM) analysis. It features a high-precision specimen feeding mechanism and a motorized sectioning system to enable consistent and reliable sectioning of samples.
Automatically generated - may contain errors
Lab products found in correlation
Jem 1400, by JEOL (1 mentions)
Aqueous uranyl acetate, by Electron Microscopy Sciences (1 mentions)
Acros organics, by Thermo Fisher Scientific (1 mentions)
Jem 1400 tem, by JEOL (1 mentions)
Talos l120c g2, by Thermo Fisher Scientific (1 mentions)
Em amw, by Leica (1 mentions)
Stemi 508, by Zeiss (1 mentions)
Jem1011 transmission electron microscope, by Nikon (1 mentions)
6 protocols using em uc7 fc7
1
Ultrastructural analysis of strawberry fruit tissues
2
Ultrastructural Analysis of Foe-TEVs
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Foe‐TEVs were fixed in 1% PFA, followed by negatively staining with 2% phosphotungstic acid. Afterward, Foe‐TEVs were placed onto a copper grid and processed for drying. Images were captured using a JEM‐1400 TEM (Jeol) an acceleration voltage of 100 kV. Renal tissues were fixed with 4% PFA and were washed gently with distilled water for several times. Then the slices were fixed with 1% OsO4 and embedded in OCT. Ultrathin slices were carried out by the LEICA EM UC7 FC7, around 80 nm thickness. Afterward, these sections were stained with lead citrate and uranium acetate. TEM was carried out through the Tecnai G2 SpiritBiotwin at 75 KV.
3
Ultrastructural Analysis of Renal Tissue
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Renal tissue was fixed with 4% PFA containing 1% glutaraldehyde and 0.1 mol/l cacodylate based buffer. Following multiple rinse cycles with double distilled water gently, the samples were dehydrated in acetone fractionation series. All sections were post-fixed with 1% OsO4 and embedded in OCT. Compound (Sakura Finetech). Ultrathin sectioning was performed with a LEICA EM UC7FC7, cutting to about 90 nm thickness. Then, the sections were stained with lead citrate as well as uranium acetate. TEM was performed with a Tecnai G2 SpiritBiotwin at 80 KV.
4
Transmission Electron Microscopy of Infected Scallop Kidneys
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Kidney samples from a subset of individuals (total of 6 scallops) confirmed to be heavily infected using the fresh preparation test (described above) were used for TEM investigations. About two cubic mm from each scallop kidney were fixed in 4% glutaraldehyde in 0.2 M cacodylate buffer (pH 7.4 adjusted to 1100 mOsmol/L with sucrose and NaCl) for 6 h at 4 °C. After fixation, tissues were washed overnight in 0.2 M cacodylate buffer containing graded concentration of sucrose. Samples were then postfixed in 1% osmium tetroxide in the same buffer for 1 h at 4 °C, rinsed with 0.2 M cacodylate buffer, dehydrated through a graded ethanol series and embedded in Epon 812. Polymerization was carried out at 60 °C for 24 h. Ultrathin serial sections were cut with an ultramicrotome (Leica EM UC7/ FC7) and were mounted on copper grids. After staining with 2% uranyl acetate for 10 min and 2% lead citrate for 3 min, the grids were examined with a JEOL 1400 transmission electron microscope.
5
Ultrastructural Analysis of Tumor Tissues
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The tumor tissues were cut into about 1 mm3 piece and fixed with 2.5 % glutaraldehyde for 6 h at 4 °C. After a serial of standard procedures of dehydration and resin-embedding, the tissue samples were sliced by ultramicrotome system (Leica, EM UC7FC7, Germany) for about 60 nm in thickness, stained with both uranyl acetate and lead citrate for 15 min before imaged using TEM (Thermo Fisher Scientific, Talos L120C G2, USA).
6
Cyclosporin C Impacts on Diamondback Moth Midgut
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Fourth instar P. xylostella larvae were treated with two concentrations of cyclosporin C (30 and 80 μg/mL) and the control treatment (ddH2O) following the method described in Section 5.2.1 . Changes in the appearance of the infected P. xylostella midgut were directly monitored at 4, 8, and 12 h post-treatment under a JEM1011 transmission electron microscope (Nikon Co. Ltd., Tokyo, Japan) following the method by Du et al. [36 ]. The treated larvae were sampled at 4, 8, and 12 h post-treatment and dissected under a stereo microscope (Stemi 508, ZEISS, Germany) to obtain midgut samples. The samples were fixed overnight in 2.5% glutaraldehyde +2% paraformaldehyde solution at 4 °C, followed by rinsing with PBS buffer (0.1 M). The samples were then stained overnight with 1% uranyl acetate at 4 °C, followed by dehydration with gradient concentrations of ethanol. The dehydrated tissues were embedded in silica gel blocks, and sections were cut using an automatic microwave tissue-processing instrument (EM AMW, Leica, Germany), and a cryo-ultramicrotome (EM UC7/FC7, Leica, Germany).
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