Log In Sign up
The largest database of trusted experimental protocols

Bicinchoninic acid assay bca assay reagents

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The Bicinchoninic acid assay (BCA assay) reagents are a set of solutions used to quantify the total protein concentration in a sample. The assay is based on the reduction of Cu2+ to Cu+ by proteins in an alkaline medium, and the subsequent colorimetric detection of the Cu+ ions using a reagent containing bicinchoninic acid.

Automatically generated - may contain errors

Lab products found in correlation

Serotonin hydrochloride, by Merck Group (3 mentions) Phenelzine, by Merck Group (3 mentions) L tryptophan, by Merck Group (3 mentions) Venlafaxine hydrochloride, by Merck Group (3 mentions) Paroxetine hydrochloride, by Merck Group (3 mentions) Citalopram hydrobromide, by Merck Group (2 mentions) 3h dihydroalprenolol, by American Radiolabeled Chemicals (2 mentions) Levo propyl 1 2 3 3h hydrochloride, by American Radiolabeled Chemicals (2 mentions) Thiazolyl blue tetrazolium bromide, by Merck Group (1 mentions) Norepinephrine hydrochloride, by Merck Group (1 mentions) Nisoxetine hydrochloride, by Merck Group (1 mentions) Sodium l ascorbate, by Merck Group (1 mentions) 3h norepinephrine, by American Radiolabeled Chemicals (1 mentions) Gbr 12935 dihydrochloride, by Merck Group (1 mentions) Decynium 22, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Dopamine hydrochloride, by Merck Group (1 mentions) Reserpine, by Merck Group (1 mentions) Cortisol, by Merck Group (1 mentions) Cimetidine hydrochloride, by Merck Group (1 mentions)

6 protocols using bicinchoninic acid assay bca assay reagents

1

Serotonin and Forskolin Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serotonin hydrochloride, L-Tryptophan, and phenelzine (MAO inhibitor) were purchased from Sigma–Aldrich (St. Louis, MO, United States). Forskolin (proliferation-activating agent) was obtained from Scintila, s.r.o. (Jihlava, CZ). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific (Waltham, MA, United States). All other chemicals were of analytical grade.
Karahoda R., Abad C., Horackova H., Kastner P., Zaugg J., Cerveny L., Kucera R., Albrecht C, & Staud F. (2020). Dynamics of Tryptophan Metabolic Pathways in Human Placenta and Placental-Derived Cells: Effect of Gestation Age and Trophoblast Differentiation. Frontiers in Cell and Developmental Biology, 8, 574034.
+ Open protocol
+ Expand
2

LPS, Poly I:C, and MTT Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lipopolysaccharides from Escherichia coli O111:B4 (LPS), polyinosinic–polycytidylic acid sodium salt (poly I:C), and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Scientific (Rockford, IL, USA). Tri Reagent solution was obtained from the Molecular Research Centre (Cincinnati, OH, USA). All other chemicals were of analytical grade.
Abad C., Karahoda R., Orbisova A., Kastner P., Heblik D., Kucera R., Portillo R, & Staud F. (2023). Pathological shifts in tryptophan metabolism in human term placenta exposed to LPS or poly I:C. Biology of Reproduction, 110(4), 722-738.
+ Open protocol
+ Expand
3

Neurotransmitter Uptake Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
3H-norepinephrine, 20 Ci/mmol, 3H-serotonin, 80 Ci/mmol, and 3H-dopamine, 60 Ci/mmol were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Dopamine hydrochloride, norepinephrine hydrochloride, serotonin hydrochloride, sodium L-ascorbate, paroxetine hydrochloride, venlafaxine hydrochloride, nisoxetine hydrochloride, GBR 12935 dihydrochloride, decynium-22 and hydrocortisone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tri Reagent solution was obtained from the Molecular Research Centre (Cincinnati, OH, USA). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific (Rockford, MA, USA). All other chemicals were of analytical grade.
Horackova H., Karahoda R., Vachalova V., Turkova H., Abad C, & Staud F. (2022). Functional characterization of dopamine and norepinephrine transport across the apical and basal plasma membranes of the human placental syncytiotrophoblast. Scientific Reports, 12, 11603.
+ Open protocol
+ Expand
4

Radioligand Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
[3H]5-hydroxytryptamine ([3H]5-HT), 80 Ci mmol−1 and [3H]dihydroalprenolol, levo-[propyl-1,2,3–3H] hydrochloride, 80 Ci mmol−1 were purchased from American Radiolabeled Chemicals, Inc. Unlabeled serotonin hydrochloride, L-tryptophan, paroxetine hydrochloride, citalopram hydrobromide, venlafaxine hydrochloride, cimetidine hydrochloride, reserpine, corticosterone, cortisol and phenelzine were purchased from Sigma-Aldrich. All other chemicals were of analytical grade. Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific. Tri Reagent solution was obtained from the Molecular Research Centre.
Karahoda R., Horackova H., Kastner P., Matthios A., Cerveny L., Kucera R., Kacerovsky M., Tebbens J.D., Bonnin A., Abad C, & Staud F. (2020). Serotonin homeostasis in the materno-foetal interface at term: Role of transporters (SERT/SLC6A4 and OCT3/SLC22A3) and monoamine oxidase A (MAO-A) in uptake and degradation of serotonin by human and rat term placenta. Acta physiologica (Oxford, England), 229(4), e13478.
+ Open protocol
+ Expand
5

Biochemical Analysis of Serotonin Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serotonin hydrochloride, L-tryptophan, and phenelzine (MAO inhibitor) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Scientific (Rockford, IL, USA). Tri Reagent solution was obtained from the Molecular Research Centre (Cincinnati, OH, USA). All other chemicals were of analytical grade.
Abad C., Karahoda R., Kastner P., Portillo R., Horackova H., Kucera R., Nachtigal P, & Staud F. (2020). Profiling of Tryptophan Metabolic Pathways in the Rat Fetoplacental Unit during Gestation. International Journal of Molecular Sciences, 21(20), 7578.
+ Open protocol
+ Expand
6

Radioligand Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
[3H]5-hydroxytryptamine ([3H]5-HT), 80 Ci/mmol; [3H]dihydroalprenolol, levo-[propyl-1,2,3-3H] hydrochloride, 80 Ci/mmol; [3H]paroxetine 20 Ci/mmol; [3H]sertraline, 80 Ci/mmol, and [3H]citalopram, 70 Ci/mmol were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Unlabeled 5-HT hydrochloride, paroxetine hydrochloride, citalopram hydrobromide, sertraline hydrochloride, fluoxetine hydrochloride, fluvoxamine maleate, and venlafaxine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific (Rockford, MA, USA) and Tri Reagent solution was obtained from the Molecular Research Centre (Cincinnati, OH, USA). All other chemicals were of analytical grade.
Horackova H., Karahoda R., Cerveny L., Vachalova V., Ebner R., Abad C, & Staud F. (2021). Effect of Selected Antidepressants on Placental Homeostasis of Serotonin: Maternal and Fetal Perspectives. Pharmaceutics, 13(8), 1306.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!