D mannitol colorimetric assay kit

Caffeine (CAF), Cytotoxicity Detection Kit (LDH) Roche, D-mannitol (MAN), D-Mannitol Colorimetric Assay Kit, disodium fumarate, deoxynivalenol (DON), L-glutamate, lucifer yellow (LY), and sodium pyruvate were obtained from Sigma Aldrich (St. Louis, MO, USA). All inorganic salts required for preparing Krebs Bicarbonate Buffer (KRB), ethanol, and glucose were purchased from Avantor (Gliwice, Poland). Amoxicillin trihydrate (AMX) and doxycycline hyclate (DOX) were generously donated by the pharmaceutical company Biofaktor Sp. z o.o. (Skierniewice, Poland) The quality of antibiotics was consistent with the monographs of the European Pharmacopoeia and corresponded to the quality of active substances used in the production of veterinary drugs.
Tissue transportation, preparation, and incubation were performed in Krebs Bicarbonate Buffer (KRB) containing 108 mM NaCl, 4.7 mM KCl, 1.8 mM Na₂HPO₄, 0.4 mM KH₂PO₄, 15 mM NaHCO₃, 1.2 mM MgSO₄, 1.25 mM CaCl₂, 11.5 mM glucose, 4.9 mM L-glutamate, 5.4 mM disodium furmate, and 4.9 mM sodium pyruvate at pH 7.4, and saturated with oxygen using a 95%/5% O₂/CO₂ mixture by gassing for 60 min [37 (link)].
For HPLC and LC-MS/MS analysis, acetonitrile, methanol, and formic acid (HPLC grade) were obtained from Avantor Chemicals (Radnor, PA, USA), trichloracetic acid and heptafluorobutyric acid were obtained from Sigma Aldrich (St. Louis, MO, USA).

CHL, D-mannitol, D-mannitol colorimetric assay kit, disodium fumarate, l-glutamate, LDH cytotoxicity detection kit, lucifer yellow (LY), and sodium pyruvate were obtained from Sigma-Aldrich (St. Louis, USA). All inorganic salts required for the preparation of Krebs-Bicarbonate Buffer, ethanol, and glucose were purchased from Avantor (Gliwice, Poland).
Purified DON (Sigma Aldrich, St. Louis, USA) was dissolved in ethanol and stored at − 20 °C before dilution in the incubation medium. MaxSignal DON ELISA Test Kit was obtained from Bioo Scientific Corporation (Texas, USA).