Anti ovalbumin igg1 mouse elisa kit

Manufactured by Cayman Chemical

Sourced in United States

The Anti-Ovalbumin IgG1 (mouse) ELISA Kit is a laboratory tool used to detect and quantify the presence of mouse IgG1 antibodies specific to ovalbumin in various sample types.

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Lab products found in correlation

Anti ovalbumin ige mouse elisa kit, by Cayman Chemical (1 mentions) Mouse ifn γ elisa kit, by Solarbio (1 mentions) Mouse il 4 elisa kit, by Solarbio (1 mentions) Ds mouse ige elisa ova, by Sumitomo Pharma (1 mentions) Opteia mouse ige elisa set, by BD (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Imject alum adjuvant, by Thermo Fisher Scientific (1 mentions) Mouse igg total elisa kit, by Thermo Fisher Scientific (1 mentions) Infinite f200 pro, by Tecan (1 mentions)

6 protocols using anti ovalbumin igg1 mouse elisa kit

Ovalbumin-Induced Allergy Biomarkers

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NALF was centrifugalized to collect the supernatant. The blood was collected from the vena cava, and then serum was collected via centrifugation. Nasal tissues of sacrificed mice were stored at −70°C, and then homogenated and centrifuged to collect the supernatant. The anti-OVA Ig (immunoglobulin) E, anti-OVA IgG1 and anti-OVA IgG1 levels in serum, and IFN-γ, IL-12, IL-4, IL-5, and IL-13 levels in the NALF and nasal mucosa were measured through enzyme-linked immunosorbent assays (ELISA) using an anti-ovalbumin IgE (mouse) ELISA kit (500840, Cayman, MI, USA), anti-ovalbumin IgG1 (mouse) ELISA kit (500830, Cayman, MI, USA), mouse anti-ovalbumin (Gal d 2) IgG2a ELISA Kit (600-120-02A, Biolead, China), mouse IFN-γ ELISA kit (SEKM-0031, Solarbio, China), mouse IL-12p40 ELISA kit (SEKM-0012, Solarbio, China), mouse IL-13 ELISA kit (SEKM-0014, Solarbio, China), mouse IL-5 ELISA kit (SEKM-0006, Solarbio, China), and mouse IL-4 ELISA kit (SEKM-0005, Solarbio, China) according to the manufacturers’ instructions.

Dong F., Tan J, & Zheng Y. (2020). Chlorogenic Acid Alleviates Allergic Inflammatory Responses Through Regulating Th1/Th2 Balance in Ovalbumin-Induced Allergic Rhinitis Mice. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e923358-1-e923358-10.

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Measuring IgE and IgG1 Levels in Mice

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At one day before the injection of OVA/Al(OH)₃, blood samples were collected from the tail vein. On the final day, whole blood was collected by cardiac puncture. The blood samples were incubated at room temperature for 1 h at 4 °C overnight and were centrifuged at 10,000 rpm for 10 min to obtain the serum. The amount of total IgE, OVA-specific IgE and IgG1 was measured in serum by using BD OptEIA Mouse IgE ELISA Set (BD Bioscience, San Jose, CA, USA), DS Mouse IgE ELISA (OVA) (DS Pharma Biomedical, Osaka, Japan), Anti-Ovalbumin IgG1 (mouse) ELISA kit (Cayman Chemical, Ann Arbor, MI, USA), respectively.

Mizuno M., Sakaguchi K, & Sakane I. (2020). Oral Administration of Fucoidan Can Exert Anti-Allergic Activity after Allergen Sensitization by Enhancement of Galectin-9 Secretion in Blood. Biomolecules, 10(2), 258.

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Immunization and Antibody Response Profiling

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Control and NHA mice were immunized with albumin from chicken egg white (OVA, Ovalbumin) from Sigma (Grade VII). Each mouse received 25 µg of OVA with Imject™ Alum Adjuvant (ALUM, Thermo Fisher Scientific) at a ratio of 1:1 in the final volume of 100 µl per mouse administered i.p. After 14 days, mice were challenged once again with the same dose of OVA-ALUM. NHA mice were divided into three groups. NHA before mice were phlebotomized before first immunization, NHA boost mice were phlebotomized before second OVA immunization, and NHA both were phlebotomized before first and second immunization (see Supplementary Fig. 3c). Untreated mice received Imject™ Alum Adjuvant without OVA. Blood was obtained from mice 14 days after the second immunization; plasma was isolated and stored at −80 °C. The concentration of anti-OVA IgG antibodies was determined using Anti-Ovalbumin IgG1 (mouse) ELISA Kit (Cayman Chemical).

Grzywa T.M., Sosnowska A., Rydzynska Z., Lazniewski M., Plewczynski D., Klicka K., Malecka-Gieldowska M., Rodziewicz-Lurzynska A., Ciepiela O., Justyniarska M., Pomper P., Grzybowski M.M., Blaszczyk R., Wegrzynowicz M., Tomaszewska A., Basak G., Golab J, & Nowis D. (2021). Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells. Communications Biology, 4, 1384.

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IgG1 Quantification from Mouse Serum

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Blood was collected by cardiac puncture at endpoints and serum isolated by coagulation and centrifugation. Serum aliquots were snap-frozen at −80° C until use. Total IgG1 was measured using IgG (Total) Mouse ELISA Kit (Thermo) and OVA-specific IgG1 was measured using Anti-Ovalbumin IgG1 (mouse) ELISA Kit (Cayman Chemical) on duplicate sera samples diluted 1:10,000 and 1:100 respectively. Absorbance values within dynamic range were read at 450 nm and 570 nm, and final concentrations were interpolated on Sigmoidal (4PL) fit of standard-curve.

Hegde S., Krisnawan V.E., Herzog B.H., Zuo C., Breden M.A., Knolhoff B.L., Hogg G.D., Tang J.P., Baer J.M., Mpoy C., Lee K.B., Alexander K.A., Rogers B.E., Murphy K.M., Hawkins W.G., Fields R.C., DeSelm C.J., Schwarz J.K, & DeNardo D.G. (2020). Dendritic cell paucity leads to dysfunctional immune surveillance in pancreatic cancer. Cancer cell, 37(3), 289-307.e9.

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Measurement of Proinflammatory Cytokines by ELISA

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Proinflammatory cytokines (TNF-α, IL-1β and IL-6) serum concentrations were measured using Enzyme-linked immunosorbent assay (ELISA) kits (ThermoFisher). Briefly, ELISA plates coated with cytokine capture antibody were washed, blocked, and incubated with 50 μl of serum samples for 2 hours. In parallel, a standard curve was made by incubating 2-fold serial dilutions of the standards. Plates were then washed and incubated with biotin-conjugated detection antibodies for 1 hour. After washing, plates were incubated with Streptavidin-HRP. Bound antibodies were detected by adding 3,3′,5,5′-tetramethylbenzidine substrate (TMB Solution) followed by plate reading for absorbance at 450 nm. Cytokines levels were calculated based on a standard curve. OVA-specific IgG1 was measured by using the anti-Ovalbumin IgG1 (mouse) ELISA Kit (#500830, CaymanChemical) according to the manufacturer’s instructions.

Murdoch D.R., Laing R.T., Mills G.D., Karalus N.C., Town G.I., Mirrett S, & Reller L.B. (2001). Evaluation of a Rapid Immunochromatographic Test for Detection of Streptococcus pneumoniae Antigen in Urine Samples from Adults with Community-Acquired Pneumonia. Journal of Clinical Microbiology, 39(10), 3495-3498.

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Quantification of Anti-OVA Antibodies

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Blood samples were collected from the tail vein of mice on day 28, and anti-OVA antibodies were detected using Anti-Ovalbumin IgG1 (mouse) ELISA Kit (Cayman Chemical, MI, United States). The antibody levels were measured according to the manufacturer’s instructions using Infinite F200 Pro microplate reader (Tecan, Grödig, Austria).

Iwashita J., Maeda H., Ishimura M, & Murata J. (2022). Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice. Frontiers in Pharmacology, 13, 851374.

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