Cy3 anti mouse igg secondary antibody

Manufactured by Jackson ImmunoResearch

Cy3-anti-mouse IgG secondary antibody is a fluorescently labeled detection reagent used to identify and visualize mouse immunoglobulin G (IgG) antibodies in various applications, such as immunohistochemistry, Western blotting, and flow cytometry. The Cy3 fluorophore, which emits in the red-orange region of the visible spectrum, is conjugated to the anti-mouse IgG antibody, enabling the detection and localization of target mouse IgG proteins.

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Lab products found in correlation

Notch1 antibody against the nicd domain, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

Cy3-conjugated goat anti-mouse secondary antibody IgG + IgM Cy3-conjugated monoclonal donkey anti-mouse IgG secondary antibody Cy3 goat anti-mouse IgG secondary antibody Cy3-conjugated donkey anti-mouse IgG secondary antibody Cy3-conjugated anti-mouse immunoglobulin G (IgG) secondary antibody Cy3-conjugated anti-mouse IgG secondary antibody Cy3 anti-mouse secondary antibody Secondary anti-mouse antibody Anti-mouse Cy3

2 protocols using cy3 anti mouse igg secondary antibody

Notch1 Activation Assay in Cells

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Subconfluent cells were treated with drug and/or adenoviral infection and fixed with 4% paraformaldehyde. The fixed cells were then treated with 0.1% Triton-100 and blocked with 10% bovine serum albumin. Cells were incubated with Notch1 antibody (against the NICD domain) (Santa Cruz Biotechnology) in 4°C overnight and stained with Cy3-anti-mouse IgG secondary antibody (Jackson ImmunoResearch). Cell nuclei were counter-stained with DAPI, followed by fluorescence microscopic imaging.

Liao J., Yu X., Hu X., Fan J., Wang J., Zhang Z., Zhao C., Zeng Z., Shu Y., Zhang R., Yan S., Li Y., Zhang W., Cui J., Ma C., Li L., Yu Y., Wu T., Wu X., Lei J., Wang J., Yang C., Wu K., Wu Y., Tang J., He B.C., Deng Z.L., Luu H.H., Haydon R.C., Reid R.R., Lee M.J., Wolf J.M., Huang W, & He T.C. (2017). lncRNA H19 mediates BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) through Notch signaling. Oncotarget, 8(32), 53581-53601.

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Notch1 Activation in Adipocyte Differentiation

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Subconfluent C3H10T_1/2 cells were infected with AdBMP9 or AdGFP. At 72 h post infection, the cells were fixed with 4% paraformaldehyde. The fixed cells were then treated with 0.1% Triton-100 and blocked with 10% bovine serum albumin. The cells were incubated with Notch1 antibody (against the NICD domain) (Santa Cruz Biotechnology) in 4 °C overnight and stained with Cy3-anti-mouse IgG secondary antibody (Jackson ImmunoResearch). Cell nuclei were counterstained with DAPI, followed by fluorescence microscopic imaging. Isotype IgG was used as a negative control. Staining reactions were done in triplicate.

Cui J., Zhang W., Huang E., Wang J., Liao J., Li R., Yu X., Zhao C., Zeng Z., Shu Y., Zhang R., Yan S., Lei J., Yang C., Wu K., Wu Y., Huang S., Ji X., Li A., Gong C., Yuan C., Zhang L., Liu W., Huang B., Feng Y., An L., Zhang B., Dai Z., Shen Y., Luo W., Wang X., Huang A., Luu H.H., Reid R.R., Wolf J.M., Thinakaran G., Lee M.J, & He T.C. (2018). BMP9-induced osteoblastic differentiation requires functional Notch signaling in mesenchymal stem cells. Laboratory Investigation; a Journal of Technical Methods and Pathology, 99(1), 58-71.

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