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2 protocols using human il 29 duoset elisa

1

Quantification of Cytokine Levels in Cell Cultures

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Cells were treated with DMSO or abemaciclib (500 nM) for 7 days. For the last 24 hours, culture media was replaced with serum free media. Following concentration of conditioned medium using Amicon Ultra centrifugal filters (Millipore), cytokines were analyzed according to the manufacturer’s recommendations: human IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience), human TNF alpha ELISA Ready-SET-Go! (Affymetrix eBioscience), Verikine human IFN alpha ELISA kit (PBL assay science), VeriKine-HS human IFN beta serum ELISA kit (PBL assay science), human IL-28B quantikine ELISA kit (R&D Systems), human IL-28A DuoSet ELISA (R&D Systems), and human IL-29 DuoSet ELISA (R&D Systems). Cytokine production by OT-I T cells in OVA/OT-I co-culture assays was measured by mouse IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience) and mouse TNF-alpha Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Anti-ANA and anti-dsDNA ELISAs (Alpha Diagnostic) were performed on plasma according to the manufacturer’s instructions. Absorbance was measured on a Synergy Neo plate reader (BioTek) using Gen5 software.
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2

Quantification of Cytokine Levels in Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were treated with DMSO or abemaciclib (500 nM) for 7 days. For the last 24 hours, culture media was replaced with serum free media. Following concentration of conditioned medium using Amicon Ultra centrifugal filters (Millipore), cytokines were analyzed according to the manufacturer’s recommendations: human IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience), human TNF alpha ELISA Ready-SET-Go! (Affymetrix eBioscience), Verikine human IFN alpha ELISA kit (PBL assay science), VeriKine-HS human IFN beta serum ELISA kit (PBL assay science), human IL-28B quantikine ELISA kit (R&D Systems), human IL-28A DuoSet ELISA (R&D Systems), and human IL-29 DuoSet ELISA (R&D Systems). Cytokine production by OT-I T cells in OVA/OT-I co-culture assays was measured by mouse IFN gamma ELISA Ready-SET-Go! (Affymetrix eBioscience) and mouse TNF-alpha Quantikine ELISA Kit (R&D Systems) according to the manufacturer’s instructions. Anti-ANA and anti-dsDNA ELISAs (Alpha Diagnostic) were performed on plasma according to the manufacturer’s instructions. Absorbance was measured on a Synergy Neo plate reader (BioTek) using Gen5 software.
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