1290 serieshigh performance liquid chromatography

Milled wheat samples
were extracted in 50 mL centrifuge tubes (Sarstedt, Nürnbrecht,
Germany) with a solid/liquid ratio (1:4, m/v) by mixing 5 g of solid
material with the extraction solvent (acetonitrile/water/acetic acid
79:20:1, v/v/v) in a GFL 3017 rotary shaker for 90 min (GFL, Burgwedel,
Germany). Thereafter, 500 μL of the extract were transferred
to autosampler vials and diluted (1:1, v/v) with the extraction solvent
(acetonitrile/water/acetic acid 79:20:1, v/v/v). After proper homogenization,
5 μL of the extract was analyzed with LC–MS/MS with a
QTrap 5500 LC–MS/MS System (Applied Biosystems, USA) provided
with TurboIonSpray electrospray ionization (ESI) source and 1290 Series
high-performance liquid chromatography (HPLC) (Agilent, Germany).
Detailed information on the instrumental conditions, calibration,
and validation of the reference method (including proficiency testing
result) is given in Sulyok et al.^{34 (link)}