The LC–MS/MS system comprises an AB Sciex API5000 Tandem Mass Spectrometer, Shimadzu Prominence 20ADXR UFLC pumps and an SIL-20ACXR autosampler managed with Analyst® 1.5.1 (AB Sciex, CA, USA). The gases for the MS system were supplied by an LC–MS gas generator (Source 5000™, Parker Balston, Inc., MA, USA). The LC columns tested include Synergi polar RP (2.0 × 50 mm, 4 µm), PolymerX RP-1 (4.0 × 50 mm, 5 µm), and pen-tafluorophenyl (PFP) (2.0 × 50 mm, 2.6 µm) columns from Phenomenex, Inc., CA, USA, and Zorbax C8 (2.1 × 50 mm, 5 µm), C18 (2.1 × 30 mm, 1.8 µm) and Pursuit PFP (2.0 × 50 mm, 3 µm) columns from Agilent Technologies, Inc., CA, USA. The LC–MS/MS system was operated in a 25°C room controlled with an air conditioner. The MS conditions for PQ and the IS were optimized by separate infusion of 50 ng/ml PQ or IS into the MS at a flow rate of 10 µl/min while adjusting MS parameters to achieve maximal signal. Ionization utilized APCL+, and detection utilized multiple reaction monitoring mode. Data were processed with Analyst 1.5.1.
Polymerx rp 1
Manufactured by Phenomenex
Sourced in United States
PolymerX RP-1 is a reversed-phase liquid chromatography column manufactured by Phenomenex. The column is designed for the separation and analysis of a wide range of organic compounds. The stationary phase consists of a polymeric material that provides a stable and durable platform for chromatographic separations.
Automatically generated - may contain errors
2 protocols using polymerx rp 1
1
LC-MS/MS Based Analytical Protocol
2
HPLC Analysis of Mycotoxin-Aluminum Complexes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mycotoxin-aluminum complexes were analyzed by HPLC using an Agilent 1200 series HPLC (Agilent Technologies GmbH, Waldbronn, Germany) consisting of an auto sampler, a binary pump, a degasser, a column oven, a diode array (DAD), and fluorescence detector (FLD). The analytical column used was a PolymerX RP-1 with dimensions of 250 × 4.6 mm, a particle size of 5 µm and a pore size of 100 Å (Phenomenex, Torrance, CA, USA) and the column oven was set to 40 °C. The mobile phase consisted of 60% acetonitrile and 40% water without modifiers for the CIT-Al HPLC method, and for the OTA-Al method, 100% of methanol were used. For both methods, the flow rate of the mobile phase was 800 µL/min, the injection volume was 20 µL, and the isocratic run was held for 15 min. A DAD scan was performed in the wavelength range of λ = 190–800 nm, and the FLD was set to excitation and emission wavelengths as follows: CIT λEx = 331 nm and λEm = 500 nm; CIT-Al complex λEx = 320 nm and λEm = 474 nm; OTA λEx = 330 nm and λEm = 465 nm; OTA-Al complex λEx = 365 nm and λEm = 425 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!