ROS production was assessed using a fluorogenic probe (CellRox Deep Red Reagent, Thermo Fisher Scientific) as described previously [24 (link)]. Adenosine triphosphate (ATP) concentration was assessed using the Colorimetric ATP Assay Kit (Abcam, Cambridge, UK). ATP generation was normalized by the number of cells. The GSH concentration was calculated using the GSH-Glo Assay kit (Promega). Cellular NADPH contents were determined by a colorimetric determination method using an NADP/NADPH determination kit (Biovision, Milpitas, CA, USA). Mitochondrial membrane potential was measured by using the JC-1 Mitochondrial Membrane Potential Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). All procedures were conducted according to the manufacturer's instructions.
Nadp nadph determination kit
Manufactured by Abcam
Sourced in United States
The NADP/NADPH determination kit is a laboratory tool used to measure the levels of NADP (nicotinamide adenine dinucleotide phosphate) and NADPH (the reduced form of NADP) in biological samples. The kit provides a colorimetric assay method for the quantitative determination of these important cofactors.
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2 protocols using nadp nadph determination kit
1
Measuring Cellular Bioenergetic Parameters
2
Intracellular NADPH Quantification
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The intracellular NADPH content was determined by the colorimetric determination method using an NADP/NADPH determination kit according to the manufacturer's manual (Biovision, Milpitas, CA, USA). T24WT, T24GR, UMUC3WT, and UMUC3GR cells (5 × 106 each) were used for NADPH determination.
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