Recombinant human growth hormone (Genotropin, somatropin 1.3 mg/4iU inj.) was obtained from Pharmacia & Upjohn Co, Div of Pfizer Inc. (NY). Bovine serum albumin was obtained from Sigma Chemical Co. (St Louis, Missouri). Dipotassium hydrogen phosphate (K2HPO4) and potassium dihydrogen phosphate (KH2PO4) were purchased from El-Nasr Chemical Co (Egypt). Glutathione peroxidase and reductase assay kit were purchased from Randox Laboratories (Antrim, UK) and Trevigen, Inc. (Gaithersburg, Maryland), respectively. Mag-NA Pure Compact RNA Isolation Kit and LightCycler_RNA Master SYBR Green I were obtained from Roche Diagnostics, (Indianapolis, Indiana)/Roche Applied Science (Mannheim, Germany). Beta-actin primer sequence design was carried out according to Peinnequin and coworkers [26 (link)], while other primer sequences were designed according to Loh and his colleagues [27 (link)]. All other chemicals and solvents were of highest-grade commercial available.
Glutathione peroxidase
Manufactured by Randox
Sourced in United Kingdom
Glutathione peroxidase is a laboratory assay that measures the enzymatic activity of glutathione peroxidase, an antioxidant enzyme. It is used to assess the level of oxidative stress in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Superoxide dismutase, by Randox (2 mentions)
Glutathione reductase, by Randox (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Magna pure compact rna isolation kit, by Roche (1 mentions)
Adiponectin, by Merck Group (1 mentions)
Leptin, by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Urea, by Pars Azmoon (1 mentions)
Ferric sulfate, by Merck Group (1 mentions)
Coomassie blue, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Ferric chloride, by Merck Group (1 mentions)
Krebs solution, by Merck Group (1 mentions)
5 protocols using glutathione peroxidase
1
Recombinant Human Growth Hormone Protocol
2
Assessing Antioxidant Enzyme Activities
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Disodium salt, hexahydrate and guanidine hydrochloride, ultrapure water, 5,5-dithiobis-2-nitrobenzoic acid (DNTB), and trichloroacetic acid were purchased from Research Organics, 4353 East 49th Street, Cleveland, Ohio 44125; superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase, catalase, total cholesterol, triglyceride, and HDL-cholesterol assay kits were purchased from Randox Laboratories Co., Antrim, UK. Adiponectin, insulin, and leptin (enzyme immunoassay kits) were products of Sigma-Aldrich Inc., St. Louis, USA. All other reagents used were products of Sigma-Aldrich Inc., St. Louis, USA.
3
Enzymatic Activity Assays for Antioxidant Proteins
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Commercial kits of superoxide dismutase and glutathione peroxidase were bought from Randox Company (Crumlin, UK) and those of AST, ALP, ALT, TG, TC, Urea, HDL, and glucose were purchased from Pars Azmoon Company (Tehran, Iran). Chemical substances including hydrogen peroxide, methanol, thiobarbituric acid, bovine serum albumin, coomassie blue, CCl4, ferric sulfate, ferric chloride, sodium acetate, and butanol were purchased from Merck Company (Germany). TPTZ and ketamine were bought from Fluka Company and Alphasan Company (Netherlands), respectively.
4
Streptozocin-Induced Oxidative Stress
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Streptozocin (STZ) was supplied by Tocris Company (London, UK). All materials of Krebs solution were obtained from Merck Company (Germany). Superoxide dismutase (SOD), glutathione peroxidase (GPX), and total antioxidant capacity (TAC) detection kits were provided by Randox (UK). Finally, lactate dehydrogenase (LDH) detection kit was obtained from Roche Diagnostics (Germany) and 8-isoprostane detection kit was purchased from Cayman Chemicals (USA).
5
Assessing Kidney Function Biomarkers
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Assay kits for kidney function parameters, glutathione peroxidase, and glutathione reductase were purchased from Randox Laboratories Limited, United Kingdom. Acetaminophen (APAP) and vitamin C were products of Emzor Pharmaceuticals, Lagos, Nigeria. The water used was glass-distilled and all other chemicals and reagents were of analytical grade.
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