Log In Sign up
The largest database of trusted experimental protocols

Ltq orbitrap discovery mass

Manufactured by Thermo Fisher Scientific
Visit Supplier

The LTQ Orbitrap Discovery mass spectrometer is a high-performance hybrid mass spectrometer that combines a linear ion trap (LTQ) with an Orbitrap mass analyzer. It provides accurate mass measurements and high-resolution mass analysis for a wide range of applications.

Automatically generated - may contain errors

Lab products found in correlation

Aqua c18 reverse phase resin, by Phenomenex (2 mentions) Partisphere strong cation exchange resin scx, by Cytiva (2 mentions) Agilent 1200 series hplc, by Agilent Technologies (2 mentions) Sequencing grade modified trypsin, by Promega (1 mentions)

3 protocols using ltq orbitrap discovery mass

1

Liquid Chromatography-Mass Spectrometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
LC-MS analysis was performed on an LTQ Orbitrap Discovery mass spectrometer (ThermoFisher) coupled to an Agilent 1200 series HPLC. Digests were pressure-loaded onto a 250-μm fused silica desalting column packed with 4 cm of Aqua C18 reverse phase resin (Phenomenex). Digests of affinity-purified samples were loaded in their entirety, whereas unfractionated sample digests were centrifuged (16,873 × g, 4 °C, 2 min) before loading half of each sample onto the column. The peptides were eluted onto a biphasic column (100-μm fused silica with a 5 μm tip, packed with 10 cm C18 and 3 cm Partisphere strong cation exchange resin (SCX, Whatman)). The peptides were eluted from the SCX onto the C18 resin and into the mass spectrometer as previously described20 (link).
Hatzios S.K., Abel S., Martell J., Hubbard T., Sasabe J., Munera D., Clark L., Bachovchin D.A., Qadri F., Ryan E.T., Davis B.M., Weerapana E, & Waldor M.K. (2016). Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nature chemical biology, 12(4), 268-274.
+ Open protocol
+ Expand
2

Liquid Chromatography-Mass Spectrometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
LC-MS analysis was performed on an LTQ Orbitrap Discovery mass spectrometer (ThermoFisher) coupled to an Agilent 1200 series HPLC. Digests were pressure-loaded onto a 250-μm fused silica desalting column packed with 4 cm of Aqua C18 reverse phase resin (Phenomenex). Digests of affinity-purified samples were loaded in their entirety, whereas unfractionated sample digests were centrifuged (16,873 × g, 4 °C, 2 min) before loading half of each sample onto the column. The peptides were eluted onto a biphasic column (100-μm fused silica with a 5 μm tip, packed with 10 cm C18 and 3 cm Partisphere strong cation exchange resin (SCX, Whatman)). The peptides were eluted from the SCX onto the C18 resin and into the mass spectrometer as previously described20 (link).
Hatzios S.K., Abel S., Martell J., Hubbard T., Sasabe J., Munera D., Clark L., Bachovchin D.A., Qadri F., Ryan E.T., Davis B.M., Weerapana E, & Waldor M.K. (2016). Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nature chemical biology, 12(4), 268-274.
+ Open protocol
+ Expand
3

Identifying Protein Ubiquitination Sites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were subjected to SDS-PAGE, followed by silver staining. The bands of interest were cut out and decolorized. After being alkylated with iodoacetamide, samples were digested with Sequencing Grade Modified Trypsin (200 ng; Promega) for 18 h at 37°C. The digested samples were analyzed on an LTQ Orbitrap Discovery mass spectrometer (Thermo Scientific), and the MASCOT software was used to determine ubiquitination sites.
Matsumoto S., Fischer E.S., Yasuda T., Dohmae N., Iwai S., Mori T., Nishi R., Yoshino K.I., Sakai W., Hanaoka F., Thomä N.H, & Sugasawa K. (2015). Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein. Nucleic Acids Research, 43(3), 1700-1713.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!