Gastrosense 750

Manufactured by PerkinElmer

Sourced in United States

The Gastrosense 750 is a laboratory instrument designed for the analysis of gastric samples. It provides accurate and reliable measurements of various parameters, enabling researchers and clinicians to assess gastrointestinal function and health.

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3 protocols using gastrosense 750

Evaluating Gastrointestinal Transit Dynamics

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To test GI transit, either 100 μl Gastrosense 750 (Perkin Elmer, USA) or 100 μl Brilliant Blue FCT (E122) solution (Langdales, UK) was administered to the stomach via gavage at 6 weeks (4 weeks post transplantation). Total GI transit time was calculated from time of administration to the first visualization of dye in the stool. Stool output in 1 h was calculated as: total number of stool in 1 h × stool weight. To assess stomach emptying and partial transit time, Gastrosense 750 fluorescence was imaged using an IVIS Lumina III In Vivo Imaging System (Perkin Elmer, USA). At 30 min, in vivo images were obtained and percentage stomach emptying calculated as:
To assess partial intestinal transit, the GI tract (stomach to terminal colon) was removed 90 min after Gastrosense 750 administration. Flourescence images were obtained of the GI tract and percentage intestinal transit calculated as:

McCann C.J., Cooper J.E., Natarajan D., Jevans B., Burnett L.E., Burns A.J, & Thapar N. (2017). Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon. Nature Communications, 8, 15937.

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Gastrosense 750 Intestinal Transit

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Male animals between 8–16 weeks were used for this assay. Animals were fasted overnight and then had free access to food and water for 1 h. 100 μl Gastrosense 750 (Perkin Elmer) prepared in PBS was administered to the stomach via gavage. To assess partial intestinal transit, the GI tract (stomach to terminal colon) was removed at indicated time. Fluorescence images were obtained of the GI tract using an IVIS Lumina II In Vivo Imaging System (Perkin Elmer).

Song Y., Fothergill L.J., Lee K.S., Liu B.Y., Koo A., Perelis M., Diwakarla S., Callaghan B., Huang J., Wykosky J., Furness J.B, & Yeo G.W. (2023). Stratification of enterochromaffin cells by single-cell expression analysis. bioRxiv.

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Imaging Gastric Uptake in Mice

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The hairs of abdominal skin of mice were removed. A total amount of 0.25 nmol imaging agent, GastroSense 750 (PerkinElmer, Inc. Waltham, MA USA) was suspended in PBS, and final volume of 200 μl of the mixture was gavaged to mice. Mice were anesthetized by inhalation of ether, and the images were taken at the time points of 0 min, 5 min, 20 min, 50 min, 80 min,110 min, and 230 min after the application of GastroSense 750 using FMT 2500 Quantitative Pre-clinical Imaging System.

Wu G., Liu Y., Liu Y., Zhang L., Zhao H., Liu L., Zhao C, & Feng W. (2018). FGF 21 deficiency slows gastric emptying and reduces initial blood alcohol concentration in mice exposed to acute alcohol in fasting state. Biochemical and biophysical research communications, 497(1), 46-50.

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