Orius sc200d camera

Manufactured by Ametek

The Orius SC200D is a scientific-grade digital camera designed for advanced microscopy and imaging applications. It features a high-resolution CMOS sensor and delivers rapid data acquisition, making it suitable for a variety of laboratory and research settings.

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Lab products found in correlation

Whatman, by Cytiva (3 mentions) Jem 2100, by JEOL (2 mentions) Digital micrograph, by Ametek (1 mentions) Ultrascan 1000xp camera, by Ametek (1 mentions) Eds detector, by JEOL (1 mentions) Talos f200x g2, by Thermo Fisher Scientific (1 mentions) Jem 2100 lab6, by JEOL (1 mentions) Whatman lter paper, by Cytiva (1 mentions)

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Orius camera (43 citations) Orius SC200D (4 citations)

6 protocols using orius sc200d camera

Characterization of Garnet-Chlorite Grain Boundary

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TEM foil location was determined via optical petrography and Scanning Electron Microscopy (SEM). Foil preparation used a Focused Ion Beam (FIB) liftout on a FEI Helios 600 NanoLab SEM following methodology similar to Wirth (2009), using an oil free high vacuum at Virginia Tech’s Nanoscale Characterization and Fabrication Laboratory (NCFL). The location of the TEM foil was marked by depositing Pt on to the sample to protect the sample from the Ga-ion beam. TEM foils (approximately 2.5 µm × 3.5 µm × 150 nm) were prepared using a Ga-ion beam, with the foil oriented normal to the garnet-chlorite grain boundary. The foil was prepared thicker than common TEM samples to mitigate sample damage in the TEM and ensure a strong diffraction contrast.
TEM analysis used a JEOL2100 TEM operated at 200 kV, with images obtained using a Gatan Ultrascan 1000XP camera. Selected Area Diffraction Patterns (SAED) were taken on ~ 150 nm radius circles. Diffraction patterns were obtained using a Gatan Orius SC200D camera and analyzed using Gatan Digital Micrograph. Electron Dispersive Spectrometry (EDS) scans of the sample utilized the Scanning Transmission Electron Microscopy mode and JEOL EDS Detector.
All crystal structure models were made using VESTA^{47 (link)}.

Nagurney A.B., Caddick M.J., Pattison D.R, & Michel F.M. (2021). Preferred orientations of garnet porphyroblasts reveal previously cryptic templating during nucleation. Scientific Reports, 11, 6869.

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TEM Analysis of Irradiated Iron Films

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TEM measurements were carried out to investigate the microstructure of both the reference and irradiated samples, revealing that the reference films are highly defected with a high density of dislocations and loops, as shown in Fig. 1. Cross-sectional TEM samples were prepared from the deposited Fe films by the focused ion beam (FIB) lift-out method. The lift-outs were created with a FEI Quanta 3D FEG Dual Beam SEM/FIB, with a Ga ion source capable of energies up to 30 kV. Final thinning was done at 2 keV to reduce the Ga beam damage before examination in the TEM. After preparation, the TEM samples were imaged using both a JEOL JEM-ARM200CF and FEI Talos F200X G2 analytical for the unirradiated samples. For the irradiated samples, the JEOL ARM200CF was used in TEM mode to measure the defect distribution, with images taken using a Gatan Orius SC200D camera and the Gatan Microscopy Suite v3.3. TEM BF images were acquired at under- and over-focused conditions with a defocus value of 2.36 μm to observe the cavities present in the samples. The under-focused images were selected to measure the size and density of the cavities. In the under-focused TEM images, cavities appear predominantly as white circles encircled by a darker Fresnel fringe. The cavity size was measured as the inner diameter of the dark fringe surrounding the white dots (39 ).

Agarwal S., Liedke M.O., Jones A.C., Reed E., Kohnert A.A., Uberuaga B.P., Wang Y.Q., Cooper J., Kaoumi D., Li N., Auguste R., Hosemann P., Capolungo L., Edwards D.J., Butterling M., Hirschmann E., Wagner A, & Selim F.A. (2020). A new mechanism for void-cascade interaction from nondestructive depth-resolved atomic-scale measurements of ion irradiation–induced defects in Fe. Science Advances, 6(31), eaba8437.

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Bioreduced Chromium Characterization

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TEM/EDS coupled with STEM (scanning transmission electron microscopy) were employed to observe the intracellular and extracellular distributions of reduced chromium phases in bioreduced samples. After complete reduction of Cr⁶⁺ (39 hrs. of incubation), a 5 mL cell-chromium suspension was taken from the 0.4 mM Cr⁶⁺ bioreduction experiment and centrifuged at 5000 g for 6 min. The resulting cell pellet was washed three times with anaerobic DI water inside a glove box to remove any excess salts. Ultrathin sections (∼50–60 nm) of the sample material were obtained by following the procedure described previously (Dong et al., 2003 ) followed by counterstaining by lead citrate. JEOL JEM-2100 LaB6 TEM with an accelerating voltage of 200 KeV fitted with STEM/EDS was employed for high resolution imaging and for compositional analysis. To identify the reduced chromium mineral, selected area electron diffraction (SAED) pattern was acquired with a Gatan Orius SC200D camera.

Singh R., Dong H., Liu D., Zhao L., Marts A.R., Farquhar E., Tierney D.L., Almquist C.B, & Briggs B.R. (2014). Reduction of hexavalent chromium by the thermophilic methanogen Methanothermobacter thermautotrophicus. Geochimica et cosmochimica acta, 148, 442-456.

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Transmission Electron Microscopy of Extracellular Vesicles

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The observation of EVs by TEM was performed as previously described (Conde Vancells et al., 2010 (link)). Briefly, the isolated EVs were resuspended in 30 μL of 2% paraformaldehyde (PFA) in PBS. 3 × 10 μL of sample were deposited on Formvar-carbon-coated copper grids. The adsorption was performed for 3 × 20 min in a wet environment and then the grids were transferred into a drop of 1% glutaraldehyde in PBS for 5 min at room. After several rinsing steps with ultrapure water, samples were contrasted for 10 or 15 min on ice with a mixture of 4% uranyl acetate and 2% methylcellulose (1:9, v/v). The excess of mixture was removed using Whatman filter paper. After drying, samples were observed under a JEOL JEM-2100 TEM at 200 kV. The acquisitions were made with Gatan Orius SC200D camera.

Song Z., Xu Y., Zhang L., Zhou L., Zhang Y., Han Y., Li X., Yu P., Qu Y., Zhao W, & Qin C. (2020). Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer’s Disease in 5XFAD Mouse Model. Frontiers in Genetics, 11, 565479.

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Extracellular Vesicle Imaging by TEM

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The observation of EVs by TEM was performed as previously described [48]. Briefly, the isolated EVs were resuspended in 30 µl of 2% paraformaldehyde (PFA) in PBS. 3 × 10 µl of sample were deposited on Formvar-carbon-coated copper grids. The adsorption was performed for 3 × 20 min in a wet environment and then the grids were transferred into a drop of 1% glutaraldehyde in PBS for 5 min at RT. After several rinsing steps with ultrapure water, samples were contrasted for 10 or 15 min on ice with a mixture of 4% uranyl acetate and 2% methylcellulose (1:9, v/v). The excess of mixture was removed using Whatman filter paper. After drying, samples were observed under a JEOL JEM-2100 TEM at 200 kV. The acquisitions were made with Gatan Orius SC200D camera.

Arab T., Raffo-Romero A., Van Camp C., Lemaire Q., Le Marrec-Croq F., Drago F., Aboulouard S., Slomianny C., Lacoste A.S., Guigon I., Touzet H., Salzet M., Fournier I., Lefebvre C., Vizioli J, & Sautière P.E. (2019). Proteomic characterisation of leech microglia extracellular vesicles (EVs): comparison between differential ultracentrifugation and Optiprep™ density gradient isolation. Journal of Extracellular Vesicles, 8(1), 1603048.

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Exosome Visualization by TEM

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Five microlitre exosome sample was deposited on Formvar-carbon-coated copper grids for 5 min at room temperature. The excess liquid was removed using Whatman lter paper. Add a drop of 2 % uranyl acetate and incubated for 1 min at room temperature. The excess liquid was removed using Whatman lter paper. After drying, samples were observed under a Tecnai G2 Spirit BioTwin TEM at 80 kV. The acquisitions were made with Gatan Orius SC200D camera.

Liu H., Zhao F., Zhang K., Zhao J., & Wang Y.N. (2021). Investigating the Immune Function and Proteomic Profiles of Plasmal Exosomes in Lactobacillus Plantarum-treated Immunosuppressive Broilers.

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