Cd8 pb rpa t8

Manufactured by BD

Sourced in United States

CD8-PB (RPA-T8) is a monoclonal antibody that binds to the CD8 antigen, which is expressed on the surface of cytotoxic T cells. This antibody is designed for use in flow cytometry applications to identify and quantify CD8+ T cells in biological samples.

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Lab products found in correlation

Tnf α pe, by BD (2 mentions) Streptavidin pacific blue, by Thermo Fisher Scientific (2 mentions) Cd49d 9f10, by BD (2 mentions) Cd3 pb sp34 2, by BD (2 mentions) Ebio64cap17, by Thermo Fisher Scientific (2 mentions) Summit data acquisition and analysis software, by Agilent Technologies (1 mentions) Ifn γ apc 4s b3, by BD (1 mentions) Cd4 bv510 l200, by BD (1 mentions) Ifn γ brilliant violet 711 4s b3, by BioLegend (1 mentions) Perforin biotinylated pf 344, by Mabtech (1 mentions) Tnf α pe cy7, by BD (1 mentions) Pf 344, by Mabtech (1 mentions) Tnf α apc, by BD (1 mentions) Rpa t8, by BD (1 mentions) Ifn γ pe 4s b3, by BD (1 mentions) Cd8 apc rpa t8, by BD (1 mentions) Cd8 pe, by BD (1 mentions) Cd4 apc, by BD (1 mentions) Pd 1 percp cy5, by BioLegend (1 mentions) Aqua or yellow live dead viability stain, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD8 PB (clone RPA-T8) RPA-T8 CD8-PB

3 protocols using cd8 pb rpa t8

Flow Cytometry Antibody Staining Protocol

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The following antibodies were used for culturing or surface and intracellular cytokine staining (ICS) for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PB (SP34-2, BD), CD4-BV510 (L200, BD), CD8-PB (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (MAb11, BD), TNF-α-PE-Cy7 (MAb11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated (anti-human IL-22, RD), Streptavidin-Pacific blue (Invitrogen), Perforin-biotinylated (Pf-344, Mabtech), Caspase 3-AF647 (C92-605,BD), and anti-Vγ2-FITC (7A5, Pierce).
After staining, the cells were fixed and subjected to flow cytometry. Lymphocytes were gated based on forward- and side-scatters, and at least 40,000 gated events were analyzed using Summit Data Acquisition and Analysis Software (DakoCytomation).

Yang E., Yang R., Guo M., Huang D., Wang W., Zhang Z., Chen C., Wang F., Ho W., Shen L., Xiao H., Chen Z.W, & Shen H. (2018). Multidrug-resistant tuberculosis (MDR-TB) strain infection in macaques results in high bacilli burdens in airways, driving broad innate/adaptive immune responses. Emerging Microbes & Infections, 7, 207.

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Multiparameter Flow Cytometry Panels

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The following Abs were used for culture or surface and intracellular cytokine staining for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PECY7 (SP34-2, BD), CD3-PB (SP34-2, BD), CD4-APC (L200, BD, used for measuring CD4 expression after CD4 depleting Ab treatment), CD8-PB (RPA-T8, BD), CD8-APC (RPA-T8, BD), CD8-PE (RPA-T8, BD), IFN-γ-Allophycocyanin (4S.B3, BD), IFN-γ-PE (4S.B3, BD), TNF-α-PE (MAb11, BD), TNF-α-APC (MAb11, BD), TNF-α-PB (MAb11, eBioscience), IL-17-PE (eBio64CAP17, eBioscience), IL-17-Alexa647 (eBio64CAP17, eBioscience), IL-22-biotinylated (anti-human IL-22, RD, see reference(44 (link))), Streptavidin-Pacific blue (invitrogen). IL-4-APC (8D4-8, eBioscience), Perforin-biotinylated (Pf-344, Mabtech)

Yao S., Huang D., Chen C.Y., Halliday L., Wang R.C, & Chen Z.W. (2014). CD4+ T cells are required to contain early extrathoracic TB dissemination and sustain multi-effector functions of CD8+ T and CD3− lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2120-2132.

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Multiparametric Flow Cytometry for Expanded TILs

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Expanded TIL were first washed in FACS Wash Buffer (Dulbecco’s phosphate-buffered saline 1X (PBS, Thermo Fisher Scientific)) with 1% bovine serum albumin (Millipore Sigma). Surface Fc receptors were blocked for 10 min at room temperature using goat serum (Sigma) diluted in FACS Wash Buffer (5%) before proceeding with surface staining on ice (100 µL per reaction) for 30 min. Cell surface expression assessment for this study was done using fluorochrome-conjugated antibodies against CD3 FITC (SK7), CD4 PerCP-Cy5.5 (RPA-T4), CD4 BUV496 (SK3), CD28 PE-Cy7 (CD28.2), CD8 PB (RPA-T8) (all BD Bioscience, San Jose, California, USA), LAG3 PE (3DS223H) (Life Technologies, Carlsbad, California, USA), PD-1 PerCP-Cy5.5 (EH12.2H7), CD27 APC (M-T271), CD8 APC-Cy7 (SK1) (Biolegend, San Diego, California, USA). Aqua or Yellow Live/Dead viability stain (Thermo Fisher Scientific) was used to exclude dead cells from analysis. Stained cells were fixed with 1% paraformaldehyde (Electron Microscope Sciences, Hatfield, Pennsylvania, USA) solution for 20 min at room temperature.

Shah P., Forget M.A., Frank M.L., Jiang P., Sakellariou-Thompson D., Federico L., Khairullah R., Neutzler C.A., Wistuba I., Chow C.W., Long Y., Fujimoto J., Lin S.Y., Maitra A., Negrao M.V., Mitchell K.G., Weissferdt A., Vaporciyan A.A., Cascone T., Roth J.A., Zhang J., Sepesi B., Gibbons D.L., Heymach J.V., Haymaker C.L., McGrail D.J., Reuben A, & Bernatchez C. (2022). Combined IL-2, agonistic CD3 and 4-1BB stimulation preserve clonotype hierarchy in propagated non-small cell lung cancer tumor-infiltrating lymphocytes. Journal for Immunotherapy of Cancer, 10(2), e003082.

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