Homogeneity of GPX4R152H was assessed by LC-MS/MS analysis using a 6520 Q-TOF mass spectrometer controlled by Agilent MassHunter software (B.05.00 version), coupled online with a 1200 series HPLC system through a Chip Cube nano-ESI interface (Agilent Technologies Italia, SpA, Italy) as described previously [30 ].
Chip cube nano esi interface
Manufactured by Agilent Technologies
Sourced in United States
The Chip Cube nano-ESI interface is a lab equipment product designed for electrospray ionization (ESI) in mass spectrometry applications. The device provides a compact and integrated solution for nano-flow sample introduction to mass spectrometers.
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3 protocols using chip cube nano esi interface
1
GPX4 Homogeneity Analysis by LC-MS/MS
2
LC-MS/MS Metabolite Profiling Protocol
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Samples were analyzed by LC-MS/MS using a 6520 Q-TOF mass spectrometer governed by Agilent MassHunter software (B.05.00 version), coupled on-line with a 1200 series HPLC system through a Chip Cube nano-ESI interface (Agilent Technologies, CA, USA). Chromatographic separations were performed on a reverse phase, high resolution 3 μm C18 Polaris chip-column (Polaris-HR-3C18, Agilent Technologies), integrating a 360 nL capacity trap-column, the column (0,75 mm × 150 mm), connection capillaries, and a nano-spray emitter.
3
LC-MS/MS Analysis of Proteins and Peptides
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Samples were analyzed by LC-MS/MS using a 6520 Q-TOF mass spectrometer controlled by Agilent MassHunter software (B.05.00 version), coupled online with a 1200 series HPLC system through a Chip Cube nano-ESI interface (Agilent Technologies, CA, USA). Chromatographic separations of intact proteins were performed on a reverse-phase, 5 μm C8 chip-column (Zorbax C8 - 0.075 mm × 40 mm, Agilent Technologies), integrating a 40 nl capacity trap-column, and a nano-spray emitter.
Peptides from tryptic digestion of GPX4 were separated on a reverse-phase, high resolution 3 μm C18 chip-column (Polaris-HR-3C18 – 0.075 mm × 150 mm, Agilent Technologies), integrating a 360 nl capacity trap-column, and a nano-spray emitter.
Peptides from tryptic digestion of GPX4 were separated on a reverse-phase, high resolution 3 μm C18 chip-column (Polaris-HR-3C18 – 0.075 mm × 150 mm, Agilent Technologies), integrating a 360 nl capacity trap-column, and a nano-spray emitter.
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