The human colon fibroblast CCD18-Co cell line was purchased from ATCC. CCD18-Co cells were grown in MEM medium (Gibco) with 10% heat-inactivated FBS, 2 mM l -glutamine, 0.1 mM non-essential amino acid (Gibco), 50 U/ml penicillin, and 50 µg/ml streptomycin. Cells were routinely confirmed to be mycoplasma-free using PCR testing [20] .
Ccd 18co cell line
Manufactured by American Type Culture Collection
Sourced in United States
The CCD-18Co cell line is a human colonic fibroblast cell line derived from normal colon tissue. This cell line is suitable for in vitro research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ccd 18co, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Non essential amino acid (neaa), by Thermo Fisher Scientific (1 mentions)
Mem medium, by Thermo Fisher Scientific (1 mentions)
Caco 2 cell line, by American Type Culture Collection (1 mentions)
Atcc crl 1459, by American Type Culture Collection (1 mentions)
Ht 29, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Crl 1459, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rapamycin, by Thermo Fisher Scientific (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Sw480, by American Type Culture Collection (1 mentions)
Sw620, by American Type Culture Collection (1 mentions)
Dld 1, by American Type Culture Collection (1 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
Hct15, by American Type Culture Collection (1 mentions)
Ls174t, by American Type Culture Collection (1 mentions)
8 protocols using ccd 18co cell line
1
Culturing Human Colon Fibroblasts
2
Culturing and Characterizing Colon Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCD-18 Co cell line (ATCC® CRL-1459™) was purchased from ATCC: The Global Bioresource Center. CCD-18 Co cell line was cultured using ATCC-formulated Eagle’s minimum essential medium with L-glutamine (catalog No. 30-2003). To make the complete growth medium, fetal bovine serum was added to a final concentration of 10%. Every two to three days, a new medium was used. The cells obtained from the patient are normal myofibroblasts in the colon. The biological safety of the CCD-18 Co cell line has been classified by the American Biosafety Association (ABSA) as level 1 (BSL-1). The CaCo-2 cell line was also purchased from ATCC and cultured according to the ATCC protocols. The CaCo-2 cell line was obtained from a patient—a 72-year-old Caucasian male diagnosed with colon adenocarcinoma. The biological safety of the obtained material is classified as level 1 (BSL-1). To make the medium complete, Eagle’s minimum essential medium with L-glutamine had fetal bovine serum added to it to a final concentration of 20%. The medium was renewed once or twice a week.
3
Culture of Normal Human Colon Cells
4
Cell Culture Protocols for CCD-18Co, HT-29, and SW620
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CCD-18Co cell line was purchased from ATCC (Manassas, VA, USA; CRL-1459) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Waltham, MA, USA). HT-29 and SW620 cell lines were purchased from the cell bank of Chinese Academy of Sciences (Shanghai, China). HT-29 cells were cultured in McCoy’s 5a medium (Gibco), and SW620 cells were cultured in DMEM (fetal bovine serum (FBS), Gibco). All cell lines were supplemented with 10% FBS (Gibco) and incubated at 37 ° C with 5% CO2 in a humidified cell culture incubator. All cell lines were routinely tested for mycoplasma contamination before use.
5
Cultivation of Human Colon Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary/early-passage normal human female colon fibroblast CCD-18Co cell line, with an approximate population doubling time of 42 hours, was purchased from the American Type Culture Collection (CRL-1459, ATCC, Maryland, USA). CCD-18Co cells were cultured in minimal essential medium (MEM, Gibco, Carlsbad, CA, USA) supplemented with 15% fetal bovine serum (FBS, Gibco), 1% nonessential amino acids (100 nmol/L, Thermo Fisher Scientific, Waltham, USA), and 100 U/mL penicillin/streptomycin (Invitrogen, CA, USA) and maintained at 37°C in humidified air with 5% CO 2 . Cells were expanded six times and then stored in liquid nitrogen or used in experiments. The cell line was tested and authenticated by short tandem repeat (STR)
6
Culturing Colon Myofibroblast Cell Line
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Myofibroblasts of the colon CCD18-Co cell line were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). CCD18-Co cells were maintained in Eagle’s minimum essential medium (EMEM) supplemented with 10% fetal bovine serum (FBS), antibiotics (streptomycin and penicillin at 100 mg/mL and 100 U/mL, respectively), 1.5 g/L sodium bicarbonate, 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, and 2 mM L-glutamine. Cells were maintained at 37 °C in the presence of 5% CO2. The range of population doubling levels (PDL) used in all experiments was from 26 to 32.
7
Colorectal Cancer Cell Line Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Colorectal cancer HT-29, LoVo, SW-480, SW-620, Caco2, HCT-116, HCT-15, RKO, LS174T and DLD-1 cell lines, and the normal colon CCD-18Co cell line were purchased from American Type Culture Collection. RPMI-1640 medium containing 2 mM L-glutamine, 10% fetal bovine serum (FBS), penicillin G (100 U/ml) and streptomycin (0.1 mg/ml) was used as cell culture medium and cultured with the cells at 37°C with 5% CO2. All culture reagents were purchased from Gibco; Thermo Fisher Scientific, Inc. HT-29 cells were inoculated and grown to 80% confluency prior to treatment with adequate concentrations of rapamycin (10 nM; cat. no. V900930; Sigma-Aldrich; Merck KGaA) and 5-amino-imidazole-4-carboxamide ribonucleotide (AICAR; 2 mM; cat. no. A9978; Sigma-Aldrich; Merck KGaA) for 90 min, and the cells were then transfected with KLK12 plasmid for 12, 24 and 48 h to detect cell viability.
8
CCD-18Co Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCD-18Co cell line was obtained from the American Type Culture Collection (ATCC; Manassas, VA). CCD-18Co cells were grown in Eagle’s Minimum Essential Medium (EMEM; Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS; Biowest, Nuaillé, France) and 1% 100 × Antibiotic–Antimycotic solution (Nacalai Tesque, Kyoto, Japan). All cells were between passages 3–5 for all experiments and maintained at 37 °C with 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!