Lungs were minced and incubated in DME + DNAse + collagenase A (Roche) rotating at 37°C for two hours. Dissociated lungs were then washed twice with PBS, and filtered through a 70 µm and 40 µm cell strainer. Cells were stained with CD45 FITC (eBioscience), CD11b PerCP-Cyanine 5.5 (eBioscience); F480 PE-Cyanine7 (Affymetrix); CCR2 APC-Cy7 (BioLegend); Ly6C e450 (Affymetrix); CD45 PE-Cyanine7 (Affymetrix); CD11b PerCP-Cyanine5.5 (eBioscience); Ly6C e450 (Affymetrix); Ly6G FITC (BioLegend); CD29 (Affymetrix) and CD24 (Affymetrix). After incubating on ice for 45 minutes, cells were washed twice in PBS with 0.1% BSA, acquired on a Fortessa (BD Biosciences) and analyzed using FACSDiva (BD Biosciences).
Cd11b percp cyanine5
Manufactured by Thermo Fisher Scientific
CD11b PerCP-Cyanine5.5 is a fluorescent-labeled antibody used in flow cytometry to detect and analyze the expression of the CD11b antigen on the surface of cells. It is a useful tool for identifying and studying myeloid cells, such as monocytes, macrophages, and neutrophils.
Automatically generated - may contain errors
Lab products found in correlation
Ly6c e450, by Thermo Fisher Scientific (1 mentions)
Cd45 fitc, by Thermo Fisher Scientific (1 mentions)
Ly6g fitc, by BioLegend (1 mentions)
Facsdiva, by BD (1 mentions)
Fortessa, by BD (1 mentions)
Cd11b percp cy5, by BD (1 mentions)
Biotin conjugated mouse lineage panel, by BD (1 mentions)
C kit pe, by BD (1 mentions)
Facsaria, by BD (1 mentions)
Cd45 fitc, by BD (1 mentions)
Ly6g apc, by Thermo Fisher Scientific (1 mentions)
Cd34 fitc, by BD (1 mentions)
Ly6g apc, by BD (1 mentions)
Sca 1 pe cy7, by BD (1 mentions)
2 protocols using cd11b percp cyanine5
1
Lung Immune Cell Isolation
2
Immune Cell Phenotyping by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To analyze neutrophils, single cell suspensions of bone marrow or peripheral blood or spleen were stained with CD11b PerCP-Cyanine5.5 (eBioscience 45-0112-82) and Ly-6G-APC (eBioscience 17-5931-82). To measure neutrophil infiltration in the lung, lung tissues were cut into very small fragments and digested by collagenase and DNase I for 20 minutes at 37°C. Single cell suspensions were then stained with CD45-FITC (BD pharmingen, 553080), Ly-6G-APC and CD11b PerCP-Cy5.5. To detect the myeloid progenitor cells, bone marrow cells were pre-stained with biotin-conjugated mouse lineage panel (BD pharmingen, 559971), and then stained with streptavidin-V450 (BD horizon, 560797), Sca-1-PE-Cy7 (BD pharmingen, 558162), c-Kit-PE (BD pharmingen, 553355 ), CD34-FITC (BD pharmingen, 560238 ) and CD16/32-APC (eBioscience, 17-0161-82). Flow cytometry was conducted on a FACS Aria (BD Biosciences).
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