G250 1

Manufactured by Agar Scientific

Sourced in United Kingdom

The G250-1 is a precision-engineered lab equipment designed for general laboratory applications. It features a robust construction and reliable performance to assist researchers and scientists in their work. The core function of the G250-1 is to provide a stable and accurate platform for various laboratory tasks and experiments.

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Lab products found in correlation

Glass microscope slide, by Thermo Fisher Scientific (1 mentions) Nanowizard 2 afm, by Bruker (1 mentions) Milli q, by Merck Group (1 mentions) Ppp nchr probe, by Nanosensors (1 mentions) Jpk data processing software, by Bruker (1 mentions)

3 protocols using g250 1

Atomic Force Microscopy of Fibril Samples

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Samples containing fibrils were diluted to give a fibril concentration of 2 μM (monomer equivalent) in 20 mM phosphate buffer at the same pH as that of the sample. Diluted samples (30–50 μl) were deposited on cleaved mica (G 250-1, Agar Scientific, Stansted, UK), which was fixed onto glass microscope slides (Thermo Scientific, Paisley, UK) and left to dry. Once dry, the slides were washed twice using 100–200 μl of double-distilled water (Elga, High Wycombe, UK) and allowed to dry before storage at room temperature (RT).
AFM images were acquired using a NanoWizard II AFM (JPK Instruments, Berlin, Germany) equipped with HQ:NSC36/No Al 65–130 kHz 0.6–2 N m^–1 chips from μmasch (Innovative Solutions Bulgaria Ltd., Sofia, Bulgaria) in intermittent-contact mode in air and processed using Gwyddion (; https://www.gwyddion.net)51 and ImageJ.52 (link)

van der Wateren I.M., Knowles T.P., Buell A.K., Dobson C.M, & Galvagnion C. (2018). C-terminal truncation of α-synuclein promotes amyloid fibril amplification at physiological pH †Electronic supplementary information (ESI) available: Supplementary methods, supplementary results, supplementary tables, supplementary figures. See DOI: 10.1039/c8sc01109e. Chemical Science, 9(25), 5506-5516.

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Immobilizing DNA-MNP Hybrid Structures on Mica

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The negatively
charged mica substrates (G250-1, Agar Scientific Ltd., Essex, U.K.)
were Ni²⁺-treated to immobilize the DNA–MNP hybrid
structures. A 20 μL aliquot of a buffered 10 mM NiCl₂ solution was deposited for 1 min onto a freshly cleaved mica substrate
fixed to a glass slide. The substrate was then thoroughly rinsed with
pure water (MilliQ, Millipore) and promptly dried with filter paper.
Subsequently, a 20 μL droplet containing the hybrid structures
was incubated for 1 min. Afterward, the sample was gently rinsed with
1 mL of imaging buffer (2 mM NiCl₂, 20 mM HEPES, pH 7.4).
Noteworthy, the hybrid structures were deposited during the first
2 h after hybridization on the same day of their preparation to avoid
cluster sedimentation.

Oropesa-Nuñez R., Zardán Gómez de la Torre T., Stopfel H., Svedlindh P., Strömberg M, & Gunnarsson K. (2020). Insights into the Formation of DNA–Magnetic Nanoparticle Hybrid Structures: Correlations between Morphological Characterization and Output from Magnetic Biosensor Measurements. ACS Sensors, 5(11), 3510-3519.

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AFM Analysis of Graphene Nanosheets

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A NanoWizard III AFM system (JPK Instruments, Berlin) was used in intermittent contact mode for the AFM measurements. Cantilevers with a nominal tip diameter of 10 nm (PPP-NCHR probe, Nanosensors) and a drive frequency of ~320 kHz were used. The images were collected with a scan rate of 0.7 Hz and a working set point above 70% of the free oscillation. The images were collected on an area of 5×5 µm 2 and have a resolution of 512×512 pixels and the corresponding height profiles were analysed with the JPK Data Processing software (JPK Instruments). A statistical analysis on 90 flakes was carried out on both sets of measurements and the data fitted with a lognormal which is an established statistic function used for fragmented systems. [148] The statistical analysis was performed by using Origin 9.1 software. The AFM measurements were carried out on drop-casted 1:30 diluted WJM-Graphene dispersion in NMP onto freshly-cleaved mica sheets (G250-1, Agar Scientific Ltd.) and dried under vacuum.

Romano V., Martín-García B., Bellani S., Marasco L., Kumar Panda J., Oropesa-Nuñez R., Najafi L., Del Rio Castillo A.E., Prato M., Mantero E., Pellegrini V., D'Angelo G, & Bonaccorso F. (2019). Flexible Graphene/Carbon Nanotube Electrochemical Double-Layer Capacitors with Ultrahigh Areal Performance. ChemPlusChem, 84(7).

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