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Cd11c apc clone n418

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CD11c APC; clone N418 is a laboratory reagent used for the identification and analysis of cells expressing the CD11c antigen. This product is intended for research use only and not for use in diagnostic procedures.

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Lab products found in correlation

Cd19 apc cy7 clone 6d5, by BioLegend (2 mentions) Cd11b sb 436 clone m1 70, by Thermo Fisher Scientific (2 mentions) Nk1.1 sb 600 clone pk136, by Thermo Fisher Scientific (2 mentions) Facs staining buffer, by Thermo Fisher Scientific (1 mentions) Macsquant analyzer machine, by Miltenyi Biotec (1 mentions) Mouse fcr blocking reagent, by Miltenyi Biotec (1 mentions) Macsquantify software, by Miltenyi Biotec (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Cd69 fitc clone h1.2f3, by Thermo Fisher Scientific (1 mentions) Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions) Cd3ε pe cy7, by BioLegend (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Anti-CD11c-APC (clone N418) CD11c (clone N418; CD11c (N418, CD11c-APC

4 protocols using cd11c apc clone n418

1

Multicolor Flow Cytometry Immunophenotyping

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FACS Staining Buffer (Thermofisher Scientific, Waltham MA, USA) was used to stain cells on ice. FC receptors (FcR) were blocked using mouse FcR blocking reagent (Miltenyi Biotec, Auburn, CA, USA) for 15 minutes protected from light followed by incubation with conjugated antibodies on ice for 30 minutes. Cells were then washed and re-suspended in FACS buffer and data was acquired using MACSQuant analyzer machine and MACSQuantify software (Miltenyi Biotech Auburn, CA, USA). Antibodies used: CD11c APC; clone N418 (Affymetrix, eBioscience, Thermofisher Scientific, Waltham MA, USA)., CD86 (B7-2) PE; clone GL1(Affymetrix, eBioscience, Thermofisher Scientific, Waltham MA, USA)., CD80 FITC; clone 16-10A1(Invitrogen, Thermofisher Scientific, Waltham MA, USA), CD4 Vioblue; clone GK1.5 (Invitrogen, Thermofisher Scientific, Waltham MA, USA), MHCII Viogreen; clone M5/114.15.2, (Miltenyi Biotech Auburn, CA, USA).
Elsayed R., Elashiry M., Liu Y., El-Awady A., Hamrick M, & Cutler C.W. (2021). Porphyromonas gingivalis Provokes Exosome Secretion and Paracrine Immune Senescence in Bystander Dendritic Cells. Frontiers in Cellular and Infection Microbiology, 11, 669989.
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2

Mitochondrial Superoxide Assessment in Cells

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Mitochondrial-specific assessment of superoxide was performed as has been previously described (Case et al., 2016 (link)). Briefly, cells were stained with cell-type specific fluorescent antibodies [anti-CD3ε PE-Cy7 (clone 145–2C11, eBioscience), CD19 APC-Cy7 (clone 6D5, BioLegend), CD11b SB-436 (clone M1/70, eBioscience), CD11c APC (clone N418, eBioscience), and NK1.1 SB-600 (clone PK136, eBioscience)] in addition to 1 µM of (MATH) O2·− -sensitive mitochondrial-localized probe, MitoSOX Red (#M36008, Thermo Fisher Scientific) for 30 min at 37 °C. Cells were analyzed on an LSRII flow cytometer at 488/610 nm excitation/emission, respectively, and data analyzed using FlowJo software.
Elkhatib S.K., Moshfegh C.M., Watson G.F, & Case A.J. (2022). T-lymphocyte tyrosine hydroxylase regulates TH17 T-lymphocytes during repeated social defeat stress. Brain, behavior, and immunity, 104, 18-28.
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3

Immunophenotyping of Murine Bone Marrow Populations

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BMMs and Flt3 L-DCs were stained with antibodies directed against murine
F4/80-APC (clone BM8), CD69-FITC (clone H1.2F3), CD11c-APC (clone N418) and
CD86-FITC (clone GL1) (eBioscience). Stainings were performed in the presence of
anti-CD16/CD32 block (2.4G2; kind gift from Louis Boon, Bioceros). Flow
cytometry was performed with LSRII (BD Biosciences), and data were analysed with
FlowJo software v.7.6.5 and v.10 (Tree Star, Inc).
Balzarolo M., Engels S., de Jong A.J., Franke K., van den Berg T.K., Gulen M.F., Ablasser A., Janssen E.M., van Steensel B, & Wolkers M.C. (2021). m6A methylation potentiates cytosolic dsDNA recognition in a sequence-specific manner. Open Biology, 11(3), 210030.
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4

Splenocyte Mitochondrial Superoxide Assay

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Freshly isolated live splenocytes were incubated in RPMI supplemented with the following fluorescently tagged antibodies targeting extracellular proteins: CD3ε PE-Cy7, CD19 APC-Cy7 (clone 6D5; BioLegend), CD11b SB-436 (clone M1/70; eBioscience), CD11c APC (clone N418; eBioscience), and NK1.1 SB-600 (clone PK136; eBioscience). Concurrently, 1 μM MitoSOX Red mitochondrial superoxide indicator (Thermo Fisher Scientific) was added, and cells were incubated for 30 minutes at 37 °C. Cells were washed and resuspended in phosphate-buffered saline, and data were acquired using a customized BD LSR II flow cytometer. MitoSOX Red mean fluorescence intensities were normalized to intraexperiment sham-operated control samples. All flow cytometry experiments were conducted with accompanying single-color and fluorescence-minus-one control tubes. Data were analyzed using FlowJo software.
Elkhatib S.K., Moshfegh C.M., Watson G.F., Schwab A.D., Katsurada K., Patel K.P, & Case A.J. (2021). Splenic Denervation Attenuates Repeated Social Defeat Stress-Induced T Lymphocyte Inflammation. Biological Psychiatry Global Open Science, 1(3), 190-200.
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