Mip 3β

Manufactured by R&D Systems

Sourced in China, United States

MIP-3β is a cytokine that belongs to the chemokine family. It is involved in the recruitment and migration of dendritic cells, lymphocytes, and other immune cells to sites of inflammation.

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Lab products found in correlation

2 protocols using mip 3β

Cytokine and Chemokine Detection in Mice

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The cytokines and chemokines of the mice were detected using corresponding commercial ELISA kits. TNF-α (Cat#: MTA00B), IL-1β (Cat#: MLB00C), IL-6 (Cat#: M6000B), IL-18 (Cat#: DY122-05), MCP-1 (Cat#: SMJE00B), CCL1 (Cat#: DY845), IL-17 (Cat#: SM1700), IL-2 (Cat#: SM2000), IL-10 (Cat#: SM1000B), IL-5 (Cat#: SM5000), MIP-2 (Cat#: SMM200), MIP-3β (Cat#: DY440), MDC (Cat#: MCC220), CX3CL1 (Cat#: MCX310), CXCL10 (Cat#: DY466-05), and CXCL12 (Cat#: DY460) ELISA kits were purchased from R&D Systems (Shanghai, China) and used according to the product specifications. An MCP-3 (Cat#: BMS6006INST) ELISA kit was acquired from Invitrogen. A GCP-2 (Cat#: ab100719) kit was obtained from Abcam. An HMGB1 ELISA kit (Cat#: 326054329) was purchased from Shino-Test Corporation (Kanagawa, Japan). A procalcitonin ELISA kit (Cat#: EF014005) was obtained from Life Sciences. All the corresponding serum was carefully stored at −80 °C in a refrigerator until it was used.

Xu M., Ge C., Qin Y., Lou D., Li Q., Feng J., Wu Y., Hu L., Wang B, & Tan J. (2020). Functional loss of inactive rhomboid-like protein 2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation. Molecular Metabolism, 34, 112-123.

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Murine GM-CSF and LPS Protocol

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Ultrapure lipopolysaccharide (LPS) from Escherichia coli 0111: B4 was obtained from Invivogen (San Diego, CA, USA). Recombinant murine GM-CSF was obtained from Peprotech (Rocky Hill, NJ, USA). The doses of both molecules were determined based on the recommendations of the manufacturer and/or through our concentration-response studies. Cytokines kits (OptEIA™ ELISA), and antibodies were purchased from eBioscience (San Diego, CA, USA) or BD Biosciences (San Jose, CA, USA). MIP-3β and RANTES were obtained from RD System (Minneapolis, MN,USA).

Carvalho-Costa T.M., Mendes M.T., da Silva M.V., da Costa T.A., Tiburcio M.G., Anhê A.C., Rodrigues V J.r, & Oliveira C.J. (2015). Immunosuppressive effects of Amblyomma cajennense tick saliva on murine bone marrow-derived dendritic cells. Parasites & Vectors, 8, 22.

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