Serum glucose was measured by an enzymatic couple method using an AU400 clinical chemistry analyzer (Beckman Coulter, Inc., Brea, CA, USA) according to the manufacturer’s instructions, with intra- and inter-assay coefficient of variation (CV) of 2.0% and 3.2%, respectively. Serum insulin was measured using a solid phase sandwich enzyme linked-immuno-sorbent assay kit procedure, (Invitrogen Human Insulin ELISA kit, Camarillo, CA, USA) according to the manufacturer’s instructions, with intra- and inter-assay CV of 5.4% and 8.5%, respectively. Insulin sensitivity was assessed using the HOMA-IR based on the formula: (insulin × glucose)/22.5 with insulin expressed as U/mL and glucose as mmol/L [28 (link)]. Beta cell function was assessed using the HOMA-B based on the formula: (20× insulin)/glucose −3.5) [29 (link)].
Au400 clinical chemistry analyzer
Manufactured by Beckman Coulter
Sourced in United States
The AU400 is a clinical chemistry analyzer manufactured by Beckman Coulter. It is designed for the analysis of various biochemical compounds in biological samples, such as blood and urine. The AU400 performs a range of clinical chemistry tests, including enzyme, substrate, and electrolyte measurements. The device utilizes photometric technology to perform these analyses.
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Lab products found in correlation
3 protocols using au400 clinical chemistry analyzer
1
Serum Glucose and Insulin Assessment
2
Serum Lipid and hsCRP Measurement
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Concentrations of serum total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol and plasma high-sensitivity C-reactive protein (hsCRP) were measured as previously described (16 (link)). Briefly, concentrations of serum HDL cholesterol were measured on an AU400 clinical chemistry analyzer (Beckman Coulter, Inc.) and LDL cholesterol concentrations were estimated with the use of the Friedewald equation (18 (link)). Concentrations of plasma hsCRP were measured by a 2-site ELISA procedure (Pig High-Sensitive CRP ELISA cat. no. KT-184; Kamiya Biomedical Company).
3
Serum Lipid and Glucose Metabolism Assessment
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