Custom colloidal silicon nitride AFM probes were constructed from tipless AFM cantilevers (spring constant 0.08 N/m; NanoAndMore USA, Soquel, CA, USA). Borosilicate microspheres (5.20 µm in diameter; Bangs Laboratories, Fishers, IN, USA) were attached near the free end of the AFM cantilever with Norland optical adhesive #81 (Norland Products, Cranbury, NJ, USA). The spherical probe serves as a model of a single asperity and as such the contact area during sliding is known (Coles et al. 2008 (link)). To ensure uniformity, cantilevers were coated with 40 nm of gold over a 5-nm adhesion layer of chromium in a thermal evaporator. The mounted probe was submerged in PBS for at least 20 min prior to imaging to allow for thermal equilibration at room temperature.
Norland optical adhesive 81
Manufactured by Norland Products
Sourced in United States
Norland Optical Adhesive 81 is a single-component, ultraviolet-curable adhesive. It is designed for bonding various materials, including glass, metals, and plastics. The adhesive cures upon exposure to ultraviolet light, forming a strong, durable bond.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti mouse igg, by Abcam (1 mentions)
Asylum research mfp3d, by Oxford Instruments (1 mentions)
No 1.5 glass coverslip, by MatTek (1 mentions)
Glass bottomed petri dish, by MatTek (1 mentions)
Polyethylene glycol peg, by Thermo Fisher Scientific (1 mentions)
Acetone, by Daejung Chemicals (1 mentions)
4 protocols using norland optical adhesive 81
1
Fabrication of Colloidal Silicon Nitride AFM Probes
2
Covalent Antibody Immobilization on AFM Beads
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
6.1 µm carboxylate beads (Polysciences, Inc, USA) were dried on a cleaned coverslip coated in tetra-chlorosilane (Gilest, USA). Using the AFM head manual z translation stage (Asylum Research MFP3D, Oxford Instruments, UK), a tipless cantilever (Nanoworld TL1-Arrow-50; Nanoworld, Switzerland) was lowered onto a droplet of UV polymerizing glue (Norland optical adhesive 81; Norland Products, USA) on the same coverslip. The cantilever was lowered onto a suitable bead and a UV flashlight was used to slightly cure the glue before raising the beaded cantilever off the surface of the coverslip. The entire chip was placed under a UV lamp for 5 minutes to cure the glue completely.
The beaded cantilever was then placed in the chip holder and onto the AFM head. The tip was then dipped into a series of four droplets on a UV-cleaned coverslip: (1) a droplet of 10 mg/mL EDAC (1-ethyl-3-(-3-dimethylaminopropyl) carbodimide; Fisher Scientific) in PBS for 2 min; (2) PBS, 2 min; (3) 33 ug/mL Goat anti-Mouse IgG-AlexaFluor488 (ab150113, Abcam) for 5 min; (4) PBS, >2 min and submerged until used. IgG was centrifuged for 5 min at 10,000 g before use in functionalization steps.
The beaded cantilever was then placed in the chip holder and onto the AFM head. The tip was then dipped into a series of four droplets on a UV-cleaned coverslip: (1) a droplet of 10 mg/mL EDAC (1-ethyl-3-(-3-dimethylaminopropyl) carbodimide; Fisher Scientific) in PBS for 2 min; (2) PBS, 2 min; (3) 33 ug/mL Goat anti-Mouse IgG-AlexaFluor488 (ab150113, Abcam) for 5 min; (4) PBS, >2 min and submerged until used. IgG was centrifuged for 5 min at 10,000 g before use in functionalization steps.
3
Hydrogel Fabrication in Custom Chambers
4
Norland Optical Adhesive 81 Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Norland Optical Adhesive 81 (NOA81) was purchased from Norland Products (Cranbury, NJ, USA), and used as received. This adhesive was reported as a mixture of mercapto-esters (50–70 wt %) and triallyl isocyanurate (30–50 wt %). Perfluoromethyldecalin (85% pure) was purchased from ABCR (Karlsruhe, Germany), acetone (99.8% pure) was purchased from Daejung (Seoul, South Korea), and polyethylene glycol (PEG) with an average molecular weight of 4000 g/mol was purchased from Fisher Chemical (Loughborough, England). All of the chemicals were used without further purification. De-ionized water (~18 MΩ) was provided from an inhouse source.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!