Qiasymphony sp automated system

Manufactured by Qiagen

Sourced in Germany

The QIAsymphony SP automated system is a fully automated platform designed for nucleic acid isolation and purification. It provides a standardized and efficient workflow for high-throughput sample processing, ensuring consistent and reliable results.

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Lab products found in correlation

Qiasymphony dsp dna midi kit, by Qiagen (3 mentions) Lightcycler 480 instrument 2, by Roche (3 mentions) Lightcycler faststart dna master hybprobe mix, by Roche (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions) Qiasymphony dsp dna kit, by Qiagen (1 mentions) Qiasymphony paxgene blood rna kit, by Qiagen (1 mentions) E gel precast agarose electrophoresis system, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

QIAsymphony (189 citations) QIAsymphony SP (99 citations) QIAsymphony SP system (21 citations) QIAsymphony system (15 citations) QIAsymphony automated system (4 citations) QIAsymphony SP automated (2 citations)

5 protocols using qiasymphony sp automated system

UGT1A1 Genotyping from Whole Blood

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For UGT1A1 genotyping purposes, DNA was extracted from whole blood, using QIAsymphony SP automated system with QIAsymphony DSP DNA Midi Kit (QIAGEN), as instructed.
Analyses were performed as described elsewhere53 (link). Primers and probes were used as 10 μM working solutions. LightCycler FastStart DNA Master HybProbe Mix (Roche) was used on a LightCycler 480 Instrument II (Roche). Alleles were determined according to the melting curves obtained.

Mölzer C., Wallner M., Kern C., Tosevska A., Schwarz U., Zadnikar R., Doberer D., Marculescu R, & Wagner K.H. (2016). Features of an altered AMPK metabolic pathway in Gilbert’s Syndrome, and its role in metabolic health. Scientific Reports, 6, 30051.

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FFPE Breast Tissue RNA Extraction

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FFPE breast tissue samples were obtained from the archives at the Department of Pathology of the Barretos Cancer Hospital. FFPE samples underwent total RNA isolation using the QIASymphony SP automated system based on magnetic-bead technology (QIAGEN, Hilden, Germany) according to the manufacturer’s protocol (RNA 130 FFPE). Quantification and RNA quality assessment were performed using a Nanodrop 2000 spectrophotometer (NanoDrop Products, Wilmington, DE, USA).

Pessôa-Pereira D., Evangelista A.F., Causin R.L., da Costa Vieira R.A., Abrahão-Machado L.F., Santana I.V., da Silva V.D., de Souza K.C., de Oliveira-Silva R.J., Fernandes G.C., Reis R.M., Palmero E.I, & Marques M.M. (2020). miRNA expression profiling of hereditary breast tumors from BRCA1- and BRCA2-germline mutation carriers in Brazil. BMC Cancer, 20, 143.

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UGT1A1 Genotyping from Whole Blood

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For UGT1A1 genotyping purposes, DNA was extracted from whole blood, using a QIAsymphony SP automated system with QIAsymphony DSP DNA Midi Kit (QIAGEN), as instructed.
Analyses were performed as described elsewhere^{70 (link)}. Primers and probes were used as 10 µM working solutions. LightCyclerFastStart DNA Master HybProbe Mix (Roche) was used on a LightCycler 480 Instrument II (Roche). Alleles were determined according to the melting curves obtained.

Mölzer C., Wallner M., Kern C., Tosevska A., Zadnikar R., Doberer D., Marculescu R, & Wagner K.H. (2017). Characteristics of the heme catabolic pathway in mild unconjugated hyperbilirubinemia and their associations with inflammation and disease prevention. Scientific Reports, 7, 755.

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DNA and RNA Extraction from Whole Blood

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Genomic DNA was extracted from 1 mL whole blood aliquots using QIAsymphony DSP DNA Kit (Qiagen) on a QIAsymphony SP automated system (Qiagen). DNA concentration and 260/280 ratio were determined using a NanoDrop Spectrophotometer (Thermo Fisher). DNA integrity was confirmed using an e-Gel Precast Agarose Electrophoresis System (Thermo Fisher) using a 1% agarose gel. RNA was extracted from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit (Qiagen) on a QIAsymphony automated system. RNA concentration and 260/280 ratio for quality control were determined using a NanoDrop Spectrophotometer.

Waksmunski A.R., Miskimen K., Song Y.E., Grunin M., Laux R., Fuzzell D., Fuzzell S., Adams L.D., Caywood L., Prough M., Stambolian D., Scott W.K., Pericak-Vance M.A, & Haines J.L. (2022). Consequences of a Rare Complement Factor H Variant for Age-Related Macular Degeneration in the Amish. Investigative Ophthalmology & Visual Science, 63(9), 8.

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Determining UGT1A1 Alleles in Whole Blood

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UGT1A1 alleles from whole blood were determined through melting curves, using QIAsymphony DSP DNA Midi Kits on a QIAsymphony SP automated system (QIAGEN). 10 μM working solutions of LightCyclerFastStart DNA Master HybProbe Mix and primers were run on a LightCycler 480 Instrument II (Roche), as described previously by von Ahsen et al. (2000) (link).

Zöhrer P.A., Hana C.A., Seyed Khoei N., Mölzer C., Hörmann-Wallner M., Tosevska A., Doberer D., Marculescu R., Bulmer A.C., Herbold C.W., Berry D, & Wagner K.H. (2021). Gilbert’s Syndrome and the Gut Microbiota – Insights From the Case-Control BILIHEALTH Study. Frontiers in Cellular and Infection Microbiology, 11, 701109.

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