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Lsrfortessa x 20 analyzer flow cytometer

Manufactured by BD

The LSRFortessa X-20 Analyzer is a flow cytometer designed for high-performance cell analysis. It is capable of detecting and analyzing multiple parameters of individual cells or particles in a fluid stream.

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2 protocols using lsrfortessa x 20 analyzer flow cytometer

1

Immune Cell Profiling in Murine PDAC

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To characterize different subpopulations of immune cells, murine orthotopic PDAC tumors were removed and single cell suspensions were obtained by digestion with Collagenase P. Cells were stained with anti-Mouse antibodies against: CD45, CD4, FoxP3, Ly-6G, Ly-6C, IFN-γ and CD8a. Sample acquisition was carried out on LSRFortessa X-20 Analyzer Flow Cytometer (BD Biosciences, Franklin Lakes, NJ). Analysis was performed with FlowJo version 10.
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2

Tumor Tissue Single-Cell Analysis

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Tumor tissues were harvested and digested into single-cell suspensions by collagenase P. The obtained cells were stained with rat antimouse CD45, CD4, Foxp3, Ly-6G, IFN-γ, and CD8a antibodies. Antibody details, including final concentrations, can be found in Table S3. Sample acquisition was performed on an LSRFortessa X-20 Analyzer flow cytometer (BD Biosciences). The analysis was performed with FlowJo version 10 software (FlowJo LLC).
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